Engineering BioBrick vectors from BioBrick parts

Reshma P Shetty, Drew Endy and Thomas F Knight Jr

Outline Background

Synthetic Biology Terms

Method Current Research Discussion

Synthetic Biology What is Synthetic Biology?

A Method Milestones

Recombinant DNA PCR(Polymerase Chain Reaction) Automated Sequencing Automated Construction Standards Abstractions

-Write

-------------------Read

Last 30 years

Synthetic Biology

Synthetic Biology (Cont.) Systems

Devices

Parts

DNA

Terms Vector Plasmid Polymerase Restriction enzyme Ligase

Method Goal

Standardize the BioBrick vectors’ assembly process Maintain

Cloning site Selection marker High copy orgin Terminators flanking the cloning site Verification primer annealing sites

Process Build new BioBrick vectors’ backbone Standard assembly for new BioBrick

Method(Cont.) Basic BioBrick

Method(Cont.) Build new BioBrick vectors’ backbone

Method(Cont.) Standard assembly for new BioBrick

Method(Cont.) Check

Cloning site Selection marker High copy orgin Terminators flanking the cloning site Verification primer annealing sites

Current Research More than 200 parts

Some Systems

Discussion Wetware

Biological film Digital counting machine

Interdisciplinary research

Computer aided design