endophytic fungi conservation: techniques, advantages, disadvantages
DESCRIPTION
Ponencia: Endophytic fungi conservation: techniques, advantages, disadvantages Autor: Dr. Gary Strobel Evento TF Innova: Workshop Biotechnology "Isolation and identification of endophytic fungi from vascular plants"TRANSCRIPT
![Page 1: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/1.jpg)
Montana State University
![Page 2: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/2.jpg)
Strobelian Advice to a Young Scientist
![Page 3: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/3.jpg)
Looking for EndophytesLooking for a Life in Science
![Page 4: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/4.jpg)
A Life in Science Has Its Many Rewards
A. Anticipation of Experimental Results
B. Some New Challenges Each Day
C. Excitement Involved in the Outcomes
D. Sharing Ideas with Students and Others
E. Feeling That You Are Contributing
![Page 5: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/5.jpg)
What Will You Do With Your Life ?
Will you live to do a job, or do a job to live ?
![Page 6: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/6.jpg)
Consider some long term goals in science
• Teaching others
• Seeing the world
• Having a livelihood
• Leaving a legacy
• Making a difference
![Page 7: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/7.jpg)
The Circle of Survival In Science
Pay Heed to It
Idea
*PR activities Preliminary Results
*Publication *Proposal /Plan
Results
*requiring help of others
![Page 8: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/8.jpg)
Advice Point-Learn to think independently
![Page 9: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/9.jpg)
Advice Point-Look around for something that is absolutely new and work on it.
1. Nothing is known about it
2. It is related to a major problem
3. It has broad significance
4. It can be approached by you
![Page 10: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/10.jpg)
The Birth of An Idea
(Identify the Problem)
1. By study and desire
2. By imagination
3. By contemplation
4. By association with other systems
5. Put the problem in your mind and let it incubate !
![Page 11: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/11.jpg)
Let’s Go Dinosaur Bone Hunting !!
![Page 12: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/12.jpg)
Be observant –work with an open unencumbered mind
![Page 13: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/13.jpg)
Advice Point: When making an important /critical observation try finding and using an independent experimental method that corroborates your 1st observation. The data should match.
![Page 14: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/14.jpg)
Scientific publication
1. Only about 1 person, on the average, will read your scientific paper.
2. About 100 will read a review article.
3. Thousands will read a popular science article.
4. Therefore continue to build a body of knowledge that will make you the expert in a field.
5. Is there a problem with this as it relates to life while at the same time having good experience in science?
![Page 15: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/15.jpg)
Doing Science in This Century Versus the LastExamine the number of authors per paper in 1920 in ScienceExamine the number of authors per paper in 2005 in Science
Doing Science Has Become a Group Effort
![Page 16: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/16.jpg)
Form your own group of collaborators
Remember you will not be asked to collaborate until you can offer something that others may not have. Build a skill set and competence !!
![Page 17: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/17.jpg)
Advice Point- Look for and find your own niche - then build on it in a concerted and strong manner, being
patient and observant.
![Page 18: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/18.jpg)
Advice Point-
Pick good colleagues
Behave professionally
Support others
Be generous
![Page 19: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/19.jpg)
Advice Point –read books on the history of science, science philosophy and works of those who have done science.
![Page 20: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/20.jpg)
Classes in Science: What can I say? There is something perverse about our educational system that stifles the
imagination and creativity. Science is not facts or vocabulary it is a process and method to make new discoveries. It is
important to think, to wonder and to be fascinated with all things around us. Try to maintain a sense of fascination with
science.
![Page 21: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/21.jpg)
General Behavior
1. Making comparisons – don’t do it !
2. Telling falsehoods- don’t do it !
3. Making failing promises- don’t do it !
4. Being egotistical- don’t do it !
5. Have realistic expectations- do it !
6. Be mannerly- do it!
![Page 22: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/22.jpg)
Advice Point- Look around and consider developing a taxonomic specialty
![Page 23: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/23.jpg)
Advice Point-don’t spend too much time in the library or at the computer. Information overload can make you feel
insignificant and unable to anything that is new or useful.
![Page 24: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/24.jpg)
Advice Point: Seek help from sagacious, experienced, respected and knowledgeable peers
![Page 25: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/25.jpg)
Advice Point- Remember some trails that you take and things that you discover in science can literally belong to you (shared with your institution and co-inventors). Intellectual property-know about it. Here are some hints on how to proceed.
![Page 26: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/26.jpg)
Keep you eyes open for things and processes that may have
potential usefulness to society. The patenting process is
recommended in order to gain ownership.
A.Filing a provisional patent
B.Company interest should be obtained
C.Filing a full patent
![Page 27: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/27.jpg)
Advice Point- Put time into others things –family, hobbies, outdoor activities, sports, keep a balance of perspective and don’t spend
too much time on one thing!! Eventually you may become non-productive or be considered –odd!!
![Page 28: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/28.jpg)
Advice Point- Develop a set of skills and techniques and cherish them and expand on them your entire life. They are the crux of your ability to do experiments and make conclusions.
![Page 29: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/29.jpg)
Advice Point- Don’t become enamored of one technique and get fixed on it!
![Page 30: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/30.jpg)
Advice Point- Mistakes, you will make them, don’t fret and stew-correct the
error and get on with life.
![Page 31: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/31.jpg)
Let Sleeping Tapirs Lie: There are always issues demanding your time- pick your issues carefully. Most are time wasters !
![Page 32: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/32.jpg)
What’s this business? Was a mistake made?
![Page 33: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/33.jpg)
The Field Experiment
![Page 34: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/34.jpg)
Black Sigatoka of Banana – Pseudomycin vs Control
![Page 35: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/35.jpg)
Advice Point-Handling the Press
![Page 36: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/36.jpg)
Advice Point-Have a weekly regime of reading science and read broadly
![Page 37: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/37.jpg)
Advice Point- Administration seems always to be calling, beware of its insidious nature. It always seems that if it were you in that position you
could do the job better. It takes you out of science in most cases.
![Page 38: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/38.jpg)
Advice Point: If your paper, grant proposal or patent is declined-DO NOT take it personally. Take what is
good about the advice offered and make the document better. Likewise be a good, kind and firm
reviewer!!
![Page 39: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/39.jpg)
Despair: Don’t do it more than two minutes! If the positive result doesn’t happen and you feel as if a frog has just slept in your mouth, things will get better. Remember you still have your brain, your hands and your heart. Get on with life-your greatest gift!
![Page 40: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/40.jpg)
BEWARE: The person whose toes you trample today
maybe connected to the face or other bodily part you
have to kiss tomorrow.
![Page 41: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/41.jpg)
On to New Ground- the Post Doc- Choose Wisely and Consider the Following:1. Ability to be mentored2. Direction that you will be given3. The employer and the situation4. Funding level and ability to network
![Page 42: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/42.jpg)
Most likely you will not end up at Yale. There will be some rough roads at lesser developed colleges, universities and other
organizations. Accept the challenge. Make something where little or nothing existed before.
![Page 43: Endophytic fungi conservation: techniques, advantages, disadvantages](https://reader034.vdocuments.site/reader034/viewer/2022052307/556fc1acd8b42ada518b48e9/html5/thumbnails/43.jpg)
Some Science Related Activities(if you do well and become the expert)
• 1.Travel to scientific meetings abroad
• 2. Advice is sought by foreign govts
• 3. Advice is requested by USA agencies
• 4. Panels and grant reviews
• 5. Company consultant activity
• 6. Guest lectures given around the globe
• 7. Congressional testimony