embryo transfer products catalogue - envimedenvimed.co.th/update0157/bioniche.pdf · embryo...

Embryo Transfer Products Catalogue

Folltropin®-V

ViGRO™ Media

SYNGRO® Media

Catheters

Collection Supplies

Embryo Freezing

Equipment

Educational Resources

06/08

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Call to Order Product:BIONICHE R EPRODUCTI V E PRODUCTS

BIONICHE A NIM A L HE A LTH

Bioniche Animal Health is a division of Bioniche

Life Sciences, a research-based Canadian

biopharmaceutical company that discovers,

develops, manufactures, and markets proprietary

products for human and animal health markets

worldwide. The company has three principle

divisions: Human Health, Animal Health, and

Food Safety. Corporate offices are in Belleville,

Ontario with research, manufacturing, and

marketing/sales facilities in Belleville, Montreal,

Quebec; Athens, Georgia; Pullman, Washington;

and Armidale, Australia.

www.bionicheanimalhealth.com

BIONICHE A NIM A L HE A LTH R EPRODUCTI V E PRODUCTS C ATA LOGUE

Key areas of strategic focus for Bioniche Life Sciences Inc. include: effective therapies for bladder cancer and potentially other cancers; a cattle vaccine to prevent E. coli O157:H7 infection in humans through contaminated food or water; and technologies to potentially replace antibiotics and improve reproduction in livestock. In 2007, Bioniche Life Sciences Inc. was recognized as one of the Top 10 Life Sciences companies in Canada.

Bioniche Animal Health brought Folltropin-V, a follicle-stimulating hormone (FSH), to the market in order to provide a high quality and consistent FSH product for Embryo Transfer (ET) practitioners. Today, Bioniche is still committed to continued research in the area of livestock reproduction and embryo transfer. Areas of research include endometritis, mastitis, non-animal origin media, semen extenders, bovine embryo vitrification, and improved synchronization protocols.

Bioniche Animal Health is pleased to have a strong relationship with universities and professional organizations and to sponsor wetlabs and embryo transfer courses around the globe. The Company has several expert consultants who are available to answer technical questions and offer advice.

In this, the 2008-2009 edition of the Bioniche Animal Health Embryo Collection and Transfer catalogue, you will find detailed information regarding our extensive line of products. If you would like additional information, please visit our website www.bionicheanimalhealth.com or contact one of the facilities listed.

SYNGRO® MEDIA 4

ViGRO™ FLUSH MEDIA 5

ViGRO™ HOLDING MEDIA 5

ViGRO™ FREEZE MEDIA 5

ViGRO™ THAW MEDIA 6

OTHER ViGRO™ MEDIA 6

EQUINE MEDIA 8

CATHETERS 9

COLLECTION SUPPLIES 9

FILTERS 10

DISHES 12

PIPETTES & TIPS 14

HANDLING SUPPLIES 16

STR AWS 17

SEALING & LABELING 19

EMBRYO TR ANSFER SUPPLIES 20

FREEZING SUPPLIES 21

GRADING, SPLITTING & SEXING EQUIPMENT 22

EMBRYO SEXING CONSUMABLES 24

EDUCATION 27

STROUD VIDEO LIBR ARY 28

1See back page for ordering details

Folltropin®-V is used for the induction of superovulation in reproductively mature heifers and cows. Folltropin®-V is highly purified, sterile, and has consistently low LH:FSH ratio.

Folltropin®-V is a highly purified extract obtained from carefully selected porcine pituitary glands. It is lyophilized to maintain potency under normal storage conditions. Each 20 mL vial contains FSH equivalent to 400 mg of NIH-FSH-P1.

* When reconstituted according to label directions, the final solution contains 20 mg/mL. Folltropin®-V Diluent is a 20 mL vial of Bacteriostatic Sodium Chloride Injection USP.# Product Description

Cat # Produc t Descr ipt ion

PHD075 Folltropin®-V (case of 48 units)

Batch after batch, vial after vial, country after country.

Reproducible Results

FOLLTROPIN ®-V

The world over,

Folltropin®-V is the

global standard in

superovulation.

The global standard of FSH excellence

Folltropsuperovand cowsterile, a

FolltropobtainedpituitarpotencyEach 20to 400 m

* When directi20 mgvial of Injecti

2Bioniche Embryo Transfer Products Catalogue

ViGRO™ media are designed specifically for the exacting needs of embryo transfer in both commercial and research applications. These media are unique because they embrace modern cell culture technology, making the entire range complete and ready to use.

Production is carried out in FDA-registered and inspected clean rooms where all media batches must pass a series of quality control tests before being transferred to inventory for sale. Furthermore, the ultra filtering process used during manufacturing ensures that no additional filtering is required before use.

Recommended storage of ViGRO™ media is between 2–8oC (except where noted).

All of these features have made ViGRO™ a benchmark for embryo transfer solutions throughout the world.

ViGRO™ MEDI A Rea gent Qua l i t y

The process begins with purified, certified chemicals. Particular attention is paid to heavy metal contamination and endotoxin levels. Protein ingredients originate from animals that were inspected both pre-and post-mortem and are free of List A diseases and transmissible spongiform encephalopathies (TSEs), including bovine spongiform encephalopathy (BSE), in accordance with OIE guidelines.

Water Qua l i t y

Water purification starts with a pre-conditioning system involving sodium ion exchange for divalent and multivalent ions, followed by 5 μm filtration and activated carbon absorption to remove chlorine, particulate matter, and other organic compounds. The pre-conditioned water then travels to a reverse osmosis (RO) unit where 98% of remaining ions, microbial impurities, and most of the remaining organics are removed. Storage of ultra-pure water in glassware and non-polyethylene containers may result in increased levels of heavy metals and/or total organic carbons within only a few days’ time. Therefore, our RO water is stored in rigid polyethylene reservoirs that will not leach impurities.

After passing though a second activated carbon absorption filter to insure removal of organics and chlorine, the water passes through a macroreticular resin to remove colloids, then passes through a two-bed cation and anion resin which removes the majority of ionic components. Finally, it passes through an ultra-pure mixed-bed cartridge, which removes all remaining ions, yielding up to 18.3 mega-ohm water, which is essentially free of ionic contaminants.

Any remaining organics are removed by using a high-efficiency synthetic carbon filter. A final 0.2 μm cross-flow filter is incorporated in the dispenser for even greater assurance of freedom from contamination.

Laborator y Procedure

The media production facility is a positive pressure, HEPA-filtered clean room equipped with Class 100 isolation areas. It is in these Class 100 isolation areas where media that has been serially filtered is packaged.

Certificates of analysis are available for each batch of ViGRO™ media.

Pack a g ing

Ethylene vinyl acetate (EVA), utilized in our media packaging, is superior to traditional plastic film because it is manufactured specifically for packaging pharmaceutical products. EVA has an ultra low level of extractibles and contains no plasticizers. The full transparency of EVA is a major advantage for visual inspection of both the solution and the empty bag.

The 8 mL tubes are made from low density polyethylene (LDPE) and have been extensively tested with bovine embryos at Colorado State University. These tubes are non-toxic, and media stored in them maintains its integrity throughout the recommended shelf life.


SY NGRO ® MEDI A

SYNGRO® Holding is the first product in a new, complete system of non-refrigerated, non-animal origin based media designed for the embryo transfer specialist. The product contains hyaluronan, a linear polysaccharide of alternating D-glucaronic acid and N-acetyl-D-glucosamine. Embryos have been shown to have surface receptors for hyaluronan, which is involved in the regulation of gene expression, cell proliferation and cell differentiation. Hyaluronan has been used very successfully as a replacement for serum or serum-based products in a number of embryo culture and freezing studies.

Practical Benefits with Every UseEliminates concerns regarding animal-based formulas.Eliminates costly air shipments of refrigerated media.Eliminates the ‘down-time’, waiting for media to warm to room temperature with each use.Eliminates wastage of ‘re-warmed’ refrigerated Holding; store extra SYNGRO® medium at room temperature.Eliminates need to change current procedures—just substitute for any holding medium now used.

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Recommended use: Embryos should be recovered from the flushing solution and transferred to a covered Petri dish containing SYNGRO® Holding. Embryos can be held at room temperature for up to 9 hours or up to 20 hours at 4°C. Embryos can be transferred using a straw containing SYNGRO® Holding.

Recommended storage: 2–30°C. DOES NOT REQUIRE REFRIGERATION.

This product does not contain materials of animal origin. # Produc t Descr ipt ion


ESM024 SYNGRO® Holding 50 mL

ESM824 SYNGRO® Holding 8 mL (sold 6 tubes per box)

SYNGRO® Holding

This kit was developed by the Equine Reproduction Laboratory at Colorado State University.

Suitable for the vitrification of equine embryos without the need for additional, expensive equipment.

Each kit comes with complete, detailed directions and contains one 8 mL vial of each of the following four media: VS1, VS2, VS3 (vitrification solutions 1, 2 and 3), & Diluent.


EQU030 SYNGRO® Equine Vitrification Kit, 4 x 8 mL vials

SYNGRO® Equine Vitrification Kit

This Vitrification Kit is based on technology developed in co-operation between Bioniche Animal Health and the Animal Reproduction and Biotechnology Laboratory at Colorado State University, Ft. Collins, Colorado.

Suitable for the vitrification of in vivo-derived bovine embryos ranging from the late morula to expanded blastocyst stage, without the need for additional, expensive equipment.

This technology is not ideally suited for cryo-preservation of in vitro-derived bovine embryos.

Each kit comes with complete, detailed directions and contains one 8 mL vial of each of the following three media: VS1, VS2, (vitrification solutions 1 and 2), & Diluent.


ESM030 SYNGRO® Bovine Vitrification Kit, 3 x 8 mL vials

SYNGRO® Bovine Vitrification Kit


The surfactant properties of proteins are provided by 1 mg/mL poly vinyl alcohol (PV-OH). Because proteins also act as heavy metal chelators, Bioniche has added other components necessary to fulfill this function.

Packaged in 1, 2 and 4 Liter EVA infusion-style bags with a total of three ports: one port for filling and two extraction ports (a needle septum port and a spike port).

Shelf life is 2 years from date of manufacture.

Recommended storage is 15–30°C; this product will not be harmed by refrigeration.

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EVM014 ViGRO™ Complete Flush Solution 1 Liter



ViGRO Complete Flush Solution

ViGRO™ FLUSH MEDI A

Holding Plus is the first bovine embryo transfer holding medium based on a formula adapted from a proven embryo culture medium. Holding Plus is designed to support optimal embryo survival in air at room temperature and provides essential amino acids, growth factors, enzymes, energy substrates and antibiotics.

Holding Plus is not an appropriate medium for long-term culture of bovine embryos in a CO

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incubator.

Packaged in EVA pouches with a needle septum port and in 8 mL disposable tubes.

Shelf life is 18 months from date of manufacture, provided sterile technique is used.

Recommended storage is 2–8°C.

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EVM024 ViGRO™ Holding Plus 50 mL

EVM224 ViGRO™ Holding Plus 20 mL

EVM824 ViGRO™ Holding Plus 8 mL (sold 6 tubes per box)

ViGRO Holding Plus

ViGRO™ HOLDING MEDI A

ViGRO™ FR EEZE MEDI A

Freeze Plus is a complete glycerol freezing solution. This medium contains 10% cell culture grade glycerol, 0.4% BSA and 0.1M sucrose. The addition of a low concentration of sucrose decreases blastomere membrane damage from osmotic stress during cryoprotectant equilibration.

Packaged in EVA pouches with a needle septum port and 8 mL disposable tubes.



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EVM032 ViGRO™ Freeze Plus, 10% Gylcerol 50 mL

EVM232 ViGRO™ Freeze Plus, 10% Glycerol 20 mL

EVM832 ViGRO™ Freeze Plus, 10% Glycerol 8 mL (sold 6 tubes per box)

ViGRO Freeze Plus, 10% Glycerol


The success and convenience of freezing embryos in ethylene glycol for direct transfer brought about a dramatic change in the ET industry. Because ethylene glycol moves across cell membranes more readily than glycerol, embryos can be transferred directly into recipients after thawing, without removing the embryo from the straw, eliminating time-consuming rehydration steps.

Ethylene Glycol Freeze Plus media with and without 0.1M sucrose both contain 1.5M ethylene glycol and 0.4% BSA.

There is no data available which indicates that either formula (with or without sucrose) is superior to the other.


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EVM034 ViGRO™ Ethylene Glycol Freeze Plus with Sucrose 50 mL

EVM234 ViGRO™ Ethylene Glycol Freeze Plus with Sucrose 20 mL

EVM834 ViGRO™ Ethylene Glycol Freeze Plus w/ Sucrose 8 mL (sold 6 tubes per box)

EVM035 ViGRO™ Ethylene Glycol Freeze Plus without Sucrose 50 mL

EVM235 ViGRO™ Ethylene Glycol Freeze Plus without Sucrose 20 mL

EVM835 ViGRO™ Ethylene Glycol Freeze Plus without Sucrose 8 mL (sold 6 tubes per box)

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ViGRO Ethylene Glycol Freeze Plus (with or without sucrose)

ViGRO™ FR EEZE MEDI A

ViGRO™ TH AW MEDI A

The use of a 1M sucrose solution for rapid one-step rehydration of embryos is convenient for thawing embryos cryopreserved in glycerol.



Recommended storage is 2-8°C.

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EVM047 ViGRO™ One-Step Thaw Plus 50 mL

EVM247 ViGRO™ One-Step Thaw Plus 20 mL

EVM847 ViGRO™ One Step Thaw Plus 8 mL (sold 6 tubes per box)

ViGRO One-Step Thaw Plus

This kit contains three solutions (Thaw 1 Plus, Thaw 2 Plus, and Thaw 3 Plus), for three-step embryo rehydration. Each solution is a complete mixture containing 0.4% BSA, decreasing concentrations of glycerol (5%, 2.5%, 0.0%) and increasing concentrations of sucrose (0.5M, 0.5M, 0.6M). This combination of glycerol, sucrose, and BSA has proven to work very well across various freezing protocols.


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EVM048 ViGRO™ Thaw Plus Kit 1,2,3 50 mL

EVM248 ViGRO™ Thaw Plus Kit 1,2,3 20 mL

EVM848 ViGRO™ Thaw Plus Kit 1,2,3 8 mL (sold 2 kits per box)

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ViGRO Thaw Plus Kit 1,2,3

OTHER ViGRO™ MEDI A

Trypsin Wash is formulated to maintain maximum cell viability while meeting IETS/OIE specifications (IETS Manual, third edition). It is a 1:250 solution of Hank’s Balanced Salts Solution(HBSS) serine proteases derived from porcine pancreas. Trypsin Wash is highly purified, virus and mycoplasma-free, and 0.22μ filtered. Packaged in biofreeze vials.

Shelf life is 9 months from date of manufacture.

This product must be shipped and stored frozen.


EVM050 ViGRO™ Trypsin Wash (.25%) 5 mL

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ViGRO Trypsin Wash (.25%)


Embryo splitting is the most effective and rapid method of increasing the number of offspring in an ET program. This medium is designed for safe and rapid splitting or biopsy without the need for a holding pipette. It contains no protein and, consequently, allows proteins in the zona pellucida to form an electrostatic attraction to the bottom of the tissue culture dish. This holds the embryo in place during the splitting procedure. Used in conjunction with Bioniche Animal Health’s Twinner System.

Packaged in EVA pouches with a needle septum port.

Shelf life is 2 years from date of manufacture, provided sterile technique is used.



EVM062 ViGRO™ Splitting Plus 50 mL

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ViGRO Splitting Plus

This is a protein-free (PF) solution which provides the “non-stick” characteristics of serum or BSA without the viral, antibody or DNA contamination problems that are associated with the use of animal-derived products. Following bisection or biopsy, a few microliters of this supplement neutralize the electrostatic attraction of the culture dish. This allows for easy recovery of demi-embryos or the risk-free transfer of biopsies to amplification capillaries. Contaminating DNA can produce false positives when using PCR diagnostic testing and this supplement eliminates the possibility of introducing bovine DNA, which is often found in FCS or BSA.

Packaged in EVA pouches with a needles septum port.

Shelf life is 2 years from date of manufacture, provided sterile technique is used.



EVM064 ViGRO™ Retrieval Supplement PF 50 mL

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ViGRO Retrieval Supplement PF

Rinsing Solution is the same formula as Complete Flush Solution, except that it does not contain any surfactant. Rinsing Solution is designed for rinsing embryo collection filters at the end of the collection with a minimum of foaming.

Packaged in 1 Liter EVA infusion-style bags with a total of three ports: one port for filling and two extraction ports (a needle septum port and a spike port).

Shelf life is 2 years from date of manufacture.


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EVM100 ViGRO™ Rinsing Solution 1 Liter

ViGRO Rinsing Solution

OTHER ViGRO™ MEDI A

TL HEPES is used for rinsing oocytes in certain steps of IVF protocols. TL HEPES is produced both with and without 0.3% BSA and is packaged in sterile 500 mL high-density PETG bottles with screw closure and a tamper-proof heat seal band.

Shelf life for both versions of TL HEPES is 1 year from date of manufacture, provided the neck seal is unbroken.

Recommended storage for TL HEPES (without BSA) is 15–30°C.

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Recommended storage for TL HEPES with BSA is 2–8°C.


IVF022 ViGRO™ TL Hepes without BSA 500 mL

IVF032 ViGRO™ TL Hepes with BSA 500 mL

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ViGRO TL Hepes (with or without BSA)


EQUINE MEDI A

This medium was developed at Colorado State University for collection of oocytes and other in vitro procedures.

Bioniche and Colorado State University modified the medium to be ideal for equine embryos.




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EQU024 Equine Holding, 50 mL

EQU224 Equine Holding, 20 mL

EQU824 Equine Holding, 8 mL (sold 6 tubes per box)

Equine Holding

This medium is appropriate for use in mares with post-foaling, bacterial, mycotic, or placental endometritis. It is also useful in pre- or post-breeding and pre- or post-insemination lavage. An iso-osmotic electrolyte solution, it is buffered to a physiological pH of 7.1 and is made from water purified by reverse osmosis to 18 megohms resistance. It contains PVA as a surfactant and is filtered to 0.2 microns. Packaged in 2 Liter, sterile, EVA infusion-style bags, it can be stored at room temperature. Shelf life is one year from the date of manufacture.

Not suitable for embryo collection.Recommended storage is 15–30°C.

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EQU100 BAH Equine Uterine Lavage 2 Liter

BAH Equine Uterine Lavage

Convenient, specialized kit that includes everything needed to flush a mare.


EQU016 Equine Embryo Recovery Kit

Equine Embryo Recovery Kit

Kit contains:1 EVM019 ViGRO Complete Flush Solution 4 Liter 1 ECE128 Equine Lavage Catheter 37 Fr. 75 cc 36 inches Autoclaved1 ECE038 Equine Y Flush Tubing with Spike1 ECE056 Millipore Non-Vented Embryo Collection Filter Gamma Irradiated2 tubes EQU824 Equine Holding Media 8 mL1 EPW022 Round Searching Grid Dish 100 x 15 mm 20/pack1 ETE044 Straws Gamma Irradiated Clear 0.5 cc 5/pack

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EVM019 ECE128 ECE038

ECE056 EQU824 EPW022 ETE044


100% pigment-free clear silicone.

Also available autoclaved.

They can be repeatedly autoclaved and reused.

The 22-inch (55.9 cm) length is 4 inches (10.2 cm) longer than a standard Foley catheter, making this ideal for uterine horn flushes of deep, mature cows.

The four openings at the distal end ensure a high flow rate and are designed to minimize the chance of the stylette slipping through a hole when repositioning the catheter.

An internal cup in the distal end accepts Bioniche’s stainless steel stylette.

When inflated, both the 30 and 5 cc cuffs (balloons) remain concentric, keeping the catheter tip free in the uterine horn lumen.

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ECE015 14 Fr. 5 cc 22 in. length











ECE120 3-Way 20 Fr. 30 cc 26 in. length

C ATHETER S

High Recovery Catheter

100% pigment-free clear silicone.

Autoclaved.

They can be repeatedly autoclaved and reused.

75 cc balloon.

Open-ended.

Available in 2 sizes

32 Fr. 34 in. (86.34 cm) length with 2 side ports.

37 Fr. 36 in. (91.44 cm) length with 3 side ports.

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ECE125 32 Fr. 75 cc 34 inches autoclaved

ECE128 37 Fr. 75 cc 36 inches autoclaved

Equine Lavage Catheter

Embryo recovery tubing set fitted with a flush solution bag connection spike, a catheter connector, and a filter connector.

There are two tubing clamps for regulating fluid flow into and out of the uterus.

Gamma sterilized.

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ECE035 Bovine Y Flush Tubing with Spike

Bovine Y Flush Tubing with Spike

This instrument is used inside bovine catheters during insertion through the cervix into the uterus.


ECE021 Stainless Steel Stylette 22.6 in. length

ECE023 Stainless Steel Stylette 26.6 in. length

BAH Stainless Steel Stylette

COLLECTION SUPPLIES


COLLECTION SUPPLIES

This is a large diameter embryo recovery tubing set for recovery of equine embryos, fitted with a flush solution bag connection spike, a catheter connector, and a filter connector.

There are two tubing clamps for regulating fluid flow into and out of the uterus.

Gamma sterilized.

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ECE038 Equine Y Flush Tubing with Spike

Equine Y Flush Tubing with Spike

Cervical dilation is sometimes needed, most frequently with heifers, to facilitate passage of the embryo recovery catheter. This stainless steel dilator is 18 inches in length and 0.25 inches in diameter, with a tapered, rounded tip.


ECE040 Cervical Dilator 18 in., 0.25 in. diameter

BAH Cervical Dilator

Vented, secure, tight-fitting lid.

150 mL capacity.

75μ nylon mesh.

Universal in-flow port.

Flow control thumb clamp.

Gamma sterilized.

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ECE050 Millipore Vented Embryo Collection Filter, Gamma Irradiated

Millipore Vented Embryo Collection Filter

Connects a luerlock syringe directly to a High Recovery Catheter for use in syringe-flushing systems.


ECE090 Catheter LuerLock Connector 1.5 inch

Catheter LuerLock Connector

FILTER S


FILTER S

High flow filter and search gridded dish all-in-one.

Filter mesh is angled along the side of the cup.


Maintains minimum 50 mL fluid level.

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ECE051 EZ Way Embryo Collection Filter, Gamma Irradiated

EZ Way Embryo Collection Filter

Vertical filter and gridded dish all-in-one.

Maintains minimum 20 mL fluid level.

Mesh size 44μ, Surface diameter 2.87 in. (73 mm).

Gamma irradiated.

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ECE052 MiniFlush Embryo Collection Filter, Gamma Irradiated

MiniFlush Embryo Collection Filter

Valve in the lid allows filter to be used in either vented or non-vented recovery systems.

150 mL capacity.

75μ steel mesh.


Gamma sterilized.

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ECE049 HY-Flow Embryo Collection Filter, Gamma Irradiated

HY-Flow Embryo Collection Filter

Non-vented, secure, tight-fitting lid.

150 mL capacity.

75μ nylon mesh.

Universal in-flow port.


Gamma sterilized.

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ECE056 Millipore Non-Vented Embryo Collection Filter, Gamma Irradiated

Millipore Non-Vented Embryo Collection Filter


DISHES

Numeric markings allow easy identification of samples.

Packaged with lid.

Gamma irradiated.

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EPW016 2-Well Cluster Dish, Round Bottom, Gamma Irradiated

2-Well Cluster Dish, Round Bottom

Polystyrene dishes with round bottom wells.

Numeric marking allow easy identification of samples.

Packaged with lid.

Gamma irradiated.

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EPW019 4-Well Mini Cluster Dish, Gamma Irradiated

4-Well Mini Cluster Dish


Packaged with lid.

Gamma irradiated.

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Packaged with lid.

Gamma irradiated.

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DISHES


EPW024 Round (Non-Gridded) Searching Dish 100 x 15 mm 20/pack

Round (Non-Gridded) Searching DishPolystyrene Petri dish with lid.


EPW030 Holding Dish Round 35 x 10 mm 10/pack Gamma Irradiated

Holding Dish, RoundIdeally suited for holding multiple embryos.


EPW040 Splitting Dish Round 60 x 15 mm 20/pack Sterile

Splitting Dish Round, SterileNon-treated, polystyrene Petri-dish.


EPW050 Embryo Searching Bowl 120 cc 10/pkg.

Embryo Searching BowlGamma irradiated.

Mouse embryo tested.

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EPW023 Square Gridded Dish 100 x 15 mm 10/pack, Sterile

Square Gridded Dish, SterilePremium quality polystyrene Petri dish with covers, featuring 13 mm grid marks. Ideal for embryo searching applications. 30% more surface area than round dishes.


EPW022 Round Searching Grid Dish 100x15 mm 20/pack

Round Searching Grid DishPolystyrene Petri dish with lid with gridded bottom ideal for embryo searching applications.

EPW040

EPW030

EPW022 EPW024

EPW023

EPW050


PIPET TES & TIPS


EHP002 0.5–10 μL Dual Control Pipette

0.5–10 μL Dual Control PipetteErgonomic design.

Shorter and more easily handled than standard pipettes, providing better control at the tip.

Variable volume (0.5–10 μL).

Durable.

Unique air vent improves precision.

Fully autoclavable.

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EHP029 2–20 μL Adjustable Pipette

2–20 μL Adjustable Pipette±4.0 to ±0.8% accuracy.

Ergonomic hand grip.

Easy-to-read display is positioned to remain visible during pipetting.

Tip ejector is removable & adjustable.

1-year warranty.

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EHP031 1 μL Embryo Handling Pipette








Embryo Handling PipetteIdeal for embryo handling.

Small, plastic, accurate and smooth to operate.

Each size will fit a variety of our pipette tips.

Tip ejectors are not included on these pipettes.

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EHP002

EHP029

EHP031-033,EHP035-039


PIPET TES & TIPS

Volume 1–200 μL.

ID: 0.012 in. (0.30 mm).

OD: 0.024 in. (0.61 mm).

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EHP051 General Purpose Tips, 204/rack; 200 μL

EHP052 General Purpose Tips, 1000 bulk; 200 μL

General Purpose Tips

Volume 2–200 μL.

Universally applicable, for use with pipettes from most manufacturers.

Tips feature fine volume graduations and a soft, flexible tip cone.

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EHP009 Eppendorf Individual Sterile Tips, 10/pack

Eppendorf Individual Sterile Tips

Volume 0.1–10 μL.

Length 1.235 in. (3.1 cm).

Ultrafine, unbeveled points.

Graduated at 2 and 10 μL.

Tips and hinged boxes are fully autoclavable to 252°F (122°C).

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EHP012 Embryo Handling Tips sterile 96/rack

Embryo Handling Tips, Sterile

Small volume gel loading tips.

Orifice dimension (outside): 0.033 in. (0.84 mm).

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EHP013 Biopsy Tips 204/rack

Biopsy Tips


PIPET TES & TIPS

Effective for embryo handling applications.

Straight cut tip.

Connect directly to 1 mL tuberculin syringes.

Semi-rigid polypropylene catheter.

Clean, non-sterile.

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EHP015 IVF Catheter, 3.5 inch, Non-Sterile, 50/pack

IVF Catheter, 3.5 inch, Non-Sterile

Connects Monoject 1 cc syringe to straw for embryo handling and straw loading applications.


C050010 Cook Syringe-Straw Connector 0.25 cc

C050011 Cook Syringe-Straw Connector 0.5 cc

Cook Syringe-Straw Connector

Ideal for use with the 0.5–10 μL Dual Control Pipette and Embryo Handling Pipettes.


EHP055 0.5–10 μL Pipette Tips 204/rack

0.5–10 μL Pipette Tips

Made from virgin polypropylene.

Precision molded for an airtight fit.

Filter inside to prevent aerosol DNA contamination of the pipette.

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EHP058 20 μL Filtered Gel Loading Tips, 204/rack, Sterile

20 μL Filtered Gel Loading Tips

H A NDLING SUPPLIES


H A NDLING SUPPLIES

PVC Straw with wick and powder, 5.25 in. (13.3 cm) length.


ETE042 Straws Non-Sterile, Clear 0.25 cc 1000/pack

Straws, Non-Sterile, Clear 0.25 cc

This adapter connects syringes to general purpose pipette tips for embryo handling.


EHP054 Syringe/Pipette Tip Connector

Syringe/Pipette Tip Connector

Disposable plastic tuberculin syringes with rubber gaskets.


GPS030 Monoject Syringe 1 cc, Sterile, 100/pack

GPS031 Monoject Syringe 1 cc, Sterile, sold individually

Monoject Syringe 1 cc, Sterile

Straws specially designed for freezing embryos.

Red, white and blue wick and powder plugs.

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ETE040 IMV Straws Gamma Irradiated, Clear 0.25 cc 5/pack

IMV Straws, Gamma Irradiated, Clear

STR AWS




ETE044 Straws Gamma Irradiated, Clear 0.5 cc 5/pack

Straws, Gamma Irradiated, Clear 0.5 cc



ETE045 Straws Non-Sterile, Clear 0.5 cc 100/pack

Straws, Non-Sterile, Clear 0.5 cc



ETE060 Straws, Gamma Irradiated 0.25 cc 5/pack

ETE063 Straws, Gamma Irradiated 0.25 cc 10/pack

Straws, Gamma Irradiated 0.25 cc

PVC Straw without wick and powder, 5.25 in. (13.3 cm) length.

Attaches to 0.25 cc straw with ETE053 adapter plug and is used as a label for the 0.25 cc straw.

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ETE055 Marking Straws, Non-Sterile Yellow 0.5 cc 100/pack

ETE056 Marking Straws, Non-Sterile Blue 0.5 cc 100/pack

Marking Straws, Non-Sterile 0.5 cc

STR AWS



Specific for embryos frozen in ethylene glycol for direct transfer (DT).

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ETE062 Straws, Gamma Irradiated, Yellow 0.25 cc 5/pack

Straws, Gamma Irradiated, Yellow 0.25 cc

Plastic I.D. plug with a 1.68 in. (42.55 mm) extension designed to fit 0.25 cc straws.


ETE084 Sealing Rods Non-Sterile Yellow 0.25 cc 100/pack

ETE085 Sealing Rods Non-Sterile White 0.25 cc 100/pack

ETE086 Sealing Rods Non-Sterile Red/Orange 0.25 cc 100/pack

ETE087 Sealing Rods Non-Sterile Blue 0.25 cc 100/pack

Sealing Rods, Non-Sterile

The small end seals 0.25 cc straws, while the opposite end accepts a 0.5 cc straw for labeling purposes.


ETE053 0.25 cc to 0.5 cc Straw Adaptor Plug 100/pack

0.25 cc to 0.5 cc Straw Adaptor Plug

Fade and water resistant ink.

Specially formulated for industrial, laboratory and commercial use.

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ETE071 Sharpie Extra Fine Point Marker Black

Sharpie Extra Fine Point Marker, Black

STR AWS

SE A LING & L A BELING


These provide a sanitary barrier as the gun traverses the vagina.

The gun is punched through the over-sheath at the entrance to the cervix.

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ETE030 Sanitary Over-Sheath 21 inches 80/roll

ETE031 Sanitary Over-Sheath 18 inches 100/roll

Sanitary Over-Sheath

EMBRYO TR A NSFER SUPPLIES

Stainless steel embryo transfer gun. Cat # Produc t Descr ipt ion

ETE010 IMV 0.25 cc Deep Chamber Gun 21 inches

IMV 0.25 cc Deep Chamber Gun

Used with IMV 0.25 cc Deep Chamber ET Gun. Cat # Produc t Descr ipt ion

ETE020 0.25 cc Blue Side-Delivery Sheath 21 inches 5/pack

0.25 cc Blue Side-Delivery Sheath

Stainless steel embryo transfer gun for 0.5 cc straws.


ETE017 IMV 0.5 cc Embryo Transfer Gun 20 inches

IMV 0.5 cc Embryo Transfer Gun


EMBRYO TR A NSFER SUPPLIES

Used with IMV 0.5 cc ET gun. Cat # Produc t Descr ipt ion

ETE018 0.5 cc Side Delivery Sheath with Chemise, Sterile

0.5 cc Side Delivery Sheath with Chemise, Sterile

Embryo transfer gun and delivery sheath all-in-one.

Sterile.

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ETE011 Disposable 0.25 cc Embryo Transfer Gun Sterile 21 inches

ETE012 Disposable 0.5 cc Embryo Transfer Gun Sterile 21 inches

Disposable Embryo Transfer Gun, Sterile

FREEZE CONTROL® systems are controlled-rate, liquid nitrogen freezers that are precision engineered for cryopreservation of biological specimens. Each modular system consists of a temperature controller, cryochamber, and cryobath. No special installation is required and systems can be quickly set up and packed away.

These patented systems provide a reliable method for freezing embryos and other biological materials for long-term preservation.


EFE011 Freeze Control CL2200 Freezer & Case



EFE016 Freeze Control CL8800i Freezer & Case

Freeze Control Freezer & Case

Aluminum canes for holding 10 mm goblets or cryo vials. Designed to hold 2 vertically-stacked goblets. Note: A single goblet per cane should be used for storing embryo straws.


EFE100 Canes for 10 mm Goblets 25/pack

Canes for 10 mm Goblets

FR EEZING SUPPLIES


FR EEZING SUPPLIES

Polyethylene 10 mm goblets for holding straws. Cat # Produc t Descr ipt ion

EFE105 Goblets, clear 10 mm 10/pack

EFE106 Goblets, blue 10 mm 10/pack

EFE108 Goblets, yellow 10 mm 10/pack

Goblets 10 mm

Aluminum identification tabs for either 10 mm or 13 mm canes.


EFE120 ID Tabs, white 100/pack

EFE122 ID Tabs, yellow 100/pack

EFE136 ID Tabs, blue 100/pack

ID Tabs

GR A DING, SPLIT TING & SE X ING EQUIPMENT

Working distance – 4 inches or 100 mmZoom magnification range – 13 to 90XField diameter – 20 to 2.9 mmInter-pupilary distance – 55 to 75 mm

This modestly priced stereo microscope is an excellent choice for embryo transfer work. It has excellent optical quality and a smooth zoom mechanism. Equipped with 20X eyepieces, the WS7 provides magnification from 13–90X. A specially designed stand with a mirror base and rear illumination system makes the WS7 ideal for grading and selecting of embryos.

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MSA060 Wesco WS7 Zoom Stereo Microscope

Wesco WS7 Zoom Stereo Microscope


MSA064 Wesco 20X 13 mm WF Eyepiece with 10/100 Micrometer

Wesco 20X 13 mm WF Eyepiece with 10/100 MicrometerUsed to measure embryo diameter during evaluation.

Primarily used for measuring equine embryos prior to cryopreservation.

Pre-installed micrometer has 10 mm/100 divisions with cross hair.

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MSA066 Wesco Stage Micrometer Scale

Wesco Stage Micrometer ScaleUsed for calibrating a microscope fitted with a micrometer eyepiece. 100 divisions. Each division is 10 microns.


This device is recommended for use with Bioniche’s Twinner and Ultra-Sharp Splitting Blades.


ESE012 Tool Holder

Tool Holder

Embryo splitting is the most effective and rapid method of increasing the number of offspring in an ET program. The Twinner is a precise system that enables trained operators to quickly and accurately biopsy and/or split both morula and blastocyst stage bovine embryos without a holding pipette.

This system consists of a high-quality, inverted microscope, a precision-crafted electronically-driven micromanipulator, and a tool holder which attaches to a Bioniche Ultra-Sharp Splitting Blade. The system is packed in a durable, foam-lined, wheeled case.


ESE008 Twinner System with Video Option

ESE010 Twinner System Binocular

Twinner System

GR A DING, SPLIT TING & SE X ING EQUIPMENT

Ophthalmic surgical blades adapted for use with the Bioniche Twinner system for embryo biopsy and/or splitting.


ESE020 Ultra-Sharp Splitting Blades 4/box

Ultra-Sharp Splitting Blades

Working distance – 4.3 inches or 110 mmZoom magnification range – 8 to 40XField of view – 37.5 to 5.5 mm

The newly developed “Comfort View” eyepiece design and the accessible, responsive controls of this microscope make operation easier and less fatiguing. The compact design, incorporating the Greenough optical system, delivers excellent flatness and ample depth of field as well as clarity, detail, and accurate color. Dependable, high-performance optics are central to the production of consistent, accurate results, as are ergonomic features which make even long-duration tasks easier and less demanding to perform.

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MSA010 Olympus SZ51 Zoom Stereo Microscope

Olympus SZ51 Zoom Stereo Microscope


EMBRYO SE X ING CONSUM A BLES

The YCD™ embryo sexing assay enables trained operators to quickly and accurately determine the sex of bovine embryos before they are transferred to recipients.

Used in conjunction with Bioniche’s Twinner and Quick-TEST systems, this cost-effective, commercially proven reagent turns embryo sexing into a simple procedure which can be completed in less than two hours. YCD is supplied in a ready-to-use formulation designed for use in a DNA amplifier. Each batch is pre-tested to ensure complete efficacy. It is manufactured with an expectation of 95% accuracy. YCD is conveniently packaged in 3,4,5, and 10 assay vials.


EST010 Bovine YCD 3 assays




EST015 Caprine YCD 3 assays




Bovine & Caprine YCD Assays

A durable, compact, portable system in which each component has been specifically selected for its suitability with Bioniche’s YCD™ embryo sexing reagent. The system includes a high quality DNA thermocycler, a gel electrophoresis unit, and an ultraviolet light source. The system is packed in a durable, foam-lined, wheeled case.


EST020 Quick-TEST

Quick-TEST

This kit contains all of the pipettes and tips for use with the Quick-TEST System.

2 x EHP051 General Purpose Tips 204/rack, 200 μL2 x EHP029 2–20 μL Adjustable Pipette2 x EHP055 0.5–10 μL Pipette Tips, 204/rack1 x EHP002 0.5–10 μL Dual Control Pipette1 x ART014 Micropipette, 100 μL1 x EHP058 20 μL Filtered Gel Loading Tips, 204/rack, Sterile


EST025 Quick-TEST Pipette Package

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Quick-TEST Pipette Package

EHP029

EHP002

ART014

EHP051 EHP055 EHP058



Diluted white blood cells which provide control samples of male and female DNA in the Bovine YCD assay.


EST030 Bovine Male Control 50 μL

EST031 Bovine Female Control 50 μL

Bovine Male/Female Control Samples

Diluted white blood cells which provide control samples of male and female DNA in the Caprine YCD assay.


EST032 Caprine Female Control 50 μL

EST033 Caprine Male Control 50 μL

Caprine Male/Female Control Samples

Thin-walled plastic tubes specifically designed for PCR.

Compatible with all major PCR machines.

Certified RNase, DNase and pyrogen free.

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MBC033 PCR Reaction Tubes 0.2 mL 1000/pack

PCR Reaction Tubes

PowerBase v.4 A compact, disposable electrophoresis unit that provides a quick and simple alternative to traditional electrophoresis units. For use with Invitrogen’s ready-to-use E-Gels.

Single Replacement Pack Invitrogen’s convenient, ready-to-use agarose gel disposable ‘cassette’ for use with the E-Gel PowerBase v.4.


EST022 E-Gel PowerBase v.4

EST027 E-Gel Single Replacement Pack

E-Gel



This kit contains all of the pipettes and tips for use with the Ampli-Y system.

2 x EHP051 General Purpose Tips 204/rack, 200 μL

1 x EHP029 2–20 μL Adjustable Pipette

2 x EHP055 0.5–10 μL Pipette Tips, 204/rack

1 x EHP002 0.5–10 μL Dual Control Pipette

1 x ART014 Micropipette, 100 μL


EST074 Ampli-Y Pipette Package

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Ampli-Y Pipette Package

A non-electrophoretic system used to determine the sex of bovine pre-implantation embryos.

The kit consists of a lysis solution (A-solution), PCR solution (B-solution), reaction tubes, and male and female control tubes.

After amplification of the samples in a thermocycler, sex is determined directly by UV illumination of the PCR tubes.

Pink fluorescence indicates the presence of a male sample.


EST070 Ampli-Y Bovine Sexing Kit (100 Reactions)

EST072 Ampli-Y Bovine Sexing Kit (25 Reactions)

Ampli-Y Bovine Sexing Kit

Protective eyewear for use when viewing a UV transilluminator.


EST041 Ultraviolet Spectacles

Ultraviolet Spectacles

EHP029

EHP002

ART014

EHP051 EHP055


EDUC ATION

Embryo SplittingThis intensive two-day course covers:

Theory of embryo splitting. Selection and maintenance of equipment.Preparation of materials.Practical training in embryo splitting and biopsy.Economic considerations.

Embryo Splitting & SexingThis intensive five-day course covers all of the Embryo Splitting material as well as:

Theory and practical training in embryo biopsy and sexing.Theory of Polymerase Chain Reaction (PCR).Selection and maintenance of equipment.Practical aspects of laboratory design.Practical training in PCR and gel electrophoresis.Freezing sexed embryos.


ETC030 Bovine Embryo Splitting Training Course

ETC040 Bovine Embryo Splitting & Sexing Training Course

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Bovine Embryo Splitting & Bovine Embryo Sexing Training CoursesThese training programs are designed to provide the participant with a complete understanding of both the theoretical and practical aspects of the techniques using the equipment and related supplies marketed by Bioniche Animal Health.


STROUD V IDEO LIBR A RY

These excellent training videos are made specifically for practitioners looking to broaden their skill range. Produced by

Dr. Brad Stroud of Stroud Veterinary Embryo Services, these videos are available in DVD format.


ETC150 Direct Transfer DVD, English, NTSC

Direct Transfer DVDRunning time: 37 minutes

This video provides a concise training tool for the practitioner new to the ‘direct transfer’ technique or training of field technicians. The utilization of skilled technicians for embryo implantation transfer is an excellent way to greatly improve the overall efficiency of any ET practice.

Also available in:NTSC; ETC060 English, PAL; ETC070 Spanish, NTSC; ETC080 Spanish, PAL.


ETC152 Bovine Reproductive Utrasonography DVD, English, NTSC

Bovine Reproductive UtrasonographyRunning time: 65 minutes

This video emphasizes fetal sexing. It also gives excellent coverage of early pregnancy diagnosis, early embryonic death, uterine and ovarian anatomy, ovarian superovulation response, ovarian cyst diagnosis and treatment evaluation, transvaginal ovum pickup and multiple pregnancy diagnosis.



ETC154 Fetal Sexing DVD, English, NTSC

Fetal Sexing DVDRunning time: 75 minutes

This video is an excellent learning tool that prepares practitioners for the mulititude of ‘less-than-ideal’ circumstances encountered on the farm. The tape also includes ‘in-field’ footage of 52 clinical examinations and offers invaluable tips on the use of trans-rectal probes and the manipulation of the gravid uterus.



To order product, please contact us at one of our locations below and ask for the order desk.

BIONICHE ANIMAL HEALTH USA, INC. (GEORGIA)1551 Jennings Mill Road, Suite 3200ABogart, Georgia 30622TEL: (706) 549-4503 (888) 549-4503FAX: (706) 548-0659

BIONICHE ANIMAL HEALTH CANADAP.O. BOX 1570Belleville, Ontario K8N 5J2TEL: (613) 966-8058 (800) 265-5464FAX: (613) 966-4177

BIONICHE ANIMAL HEALTH USA, INC. (WASHINGTON)1335 NE Terre View DrivePullman, Washington 99163TEL: (509) 335-4047 (800) 335-8595FAX: (509) 335-1064

BIONICHE ANIMAL HEALTH (EUROPE) LTD.Unit 80, Bracetown Business ParkClonee, Co MeathIrelandTEL: +353 1801 4000FAX: +353 1801 4100

BIONICHE ANIMAL HEALTHA/ASIA PTY. LTD.242 Hawthorn Road,Caulfield, Victoria, Australia 3161TEL: 1 800-032-355 (03) 9524-3161FAX: (03) 9524-3111


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