efficacy of equine oral joint...

Few studies have examined the efficacy of glucosamine and chondroitin sulfate supplements in horses. The purpose of this study is to determine if the commercially available supplements are meeting the label guarantees, and to analyze any correlation between price point and efficacy of use. The supplemental ingredients of interest to this study are combinations of glucosamine and chondroitin sulfate. Horses in the study will be fed each supplement for a 14-day period, with synovial fluid extracted through aseptic arthrocentesis at days 0 and 14. Enzyme-linked immunosorbent assays will be used to quantify the amounts of hyaluronic acid and chondroitin sulfate in each sample. Fluorophore assisted carbohydrate electrophoresis will be employed for quantification of glucosamine. Methods Twelve horses will be fed supplements according to product recommendations and monitored for a 14-day period. Prior to each feeding period, the soundness of each horse will be documented with a standard lameness evaluation at the walk and the trot in addition to flexion tests of each leg. Serum and synovial fluid will be collected for further analysis. At the end of the 14-day feeding period, soundness will again be evaluated and serum and synovial fluid samples collected. Three 14-day feeding rotations with 28-day intervals between will allow each of the 12 horses to be evaluated after consuming each of the three supplements. The synovial fluid will be extracted by a licensed veterinarian using surgical preparatory sterilization techniques. Synovial fluid will be extracted from the left fetlock joint. If the sufficient quantities of fluid cannot be extracted from that specific joint, or sample contamination occurs, then fluid from the right fetlock, the left tibial- tarsal joint, and the right tibial-tarsal joint in that specific order will extracted based on quantity obtained from each joint and any contamination of the sample. Sedatives will be used on an individual basis. The samples will be analyzed using fluorophore-assisted carbohydrate electrophoresis (FACE) for glucosamine and enzyme-linked immunosorbent assays (ELISA) for the hyaluronic acid and chondroitin sulfate. Efficacy of Equine Oral Joint Supplements Erika M. Wooten J.M. Mehaffey, Sarah Canterberry, Derek Grant, Stacie Appleton, Leon Young Erika Wooten is pursuing a Master of Science in Agriculture at Stephen F. Austin State University. She received her Bachelor of Science in Animal Science from Stephen F. Austin State University.

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Page 1: Efficacy of Equine Oral Joint Supplementsag.sfasu.edu/images/files/document/ThesisOverview_update.pdfused to quantify the amounts of hyaluronic acid and chondroitin sulfate in each

Twelvehorseswillbefedsupplementsaccordingtoproductrecommendationsandmonitoredfora14-dayperiod.Priortoeachfeedingperiod,thesoundnessofeachhorsewillbedocumentedwithastandardlamenessevaluationatthewalkandthetrotinadditiontoflexiontestsofeachleg.Serumandsynovialfluidwillbecollectedforfurtheranalysis.Attheendofthe14-dayfeedingperiod,soundnesswillagainbeevaluatedandserumandsynovialfluidsamplescollected.Three14-dayfeedingrotationswith28-dayintervalsbetweenwillalloweachofthe12horsestobeevaluatedafterconsumingeachofthethreesupplements.Thesynovialfluidwillbeextractedbyalicensedveterinarianusingsurgicalpreparatorysterilizationtechniques.Synovialfluidwillbeextractedfromtheleftfetlockjoint.Ifthesufficientquantitiesoffluidcannotbeextractedfromthatspecificjoint,orsamplecontaminationoccurs,thenfluidfromtherightfetlock,thelefttibial-tarsaljoint,andtherighttibial-tarsaljointinthatspecificorderwillextractedbasedonquantityobtainedfromeachjointandanycontaminationofthesample.Sedatives

willbeusedonanindividualbasis.Thesampleswillbeanalyzedusingfluorophore-assistedcarbohydrateelectrophoresis(FACE)forglucosamineandenzyme-linkedimmunosorbentassays(ELISA)forthehyaluronicacidandchondroitinsulfate.

Efficacy of Equine Oral Joint Supplements Erika M. Wooten J.M. Mehaffey, Sarah Canterberry, Derek Grant, Stacie Appleton, Leon Young

ErikaWootenispursuingaMasterofScienceinAgricultureatStephenF.AustinStateUniversity.ShereceivedherBachelorofScienceinAnimalSciencefromStephenF.AustinStateUniversity.

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