effects of fruit extract on growth inhibition
TRANSCRIPT
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Gleditsia sinensis fruit extract induced growth inhibition
involves basic fibrobast growth factor and nitric oxide
CHUI, C.H. et al.
Várias instituições da China.
Int. J. Mol. Med. 13: 169-73, 2004.
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INTRODUÇÃO
• G. sinensis– Medicamento utilizado na China
continental;– Atividade citotóxica contra várias
linhagens tumorais;– Induz apoptose em células leucêmicas.
• Outros extratos inibição de crescimento.
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INTRODUÇÃO
• bFGF– Angiogênese;
– Mitose;– Inibição de crescimento de câncer de mama.
• NO– xNOS;– Função biológica.
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INTRODUÇÃO
• OBJETIVOS– Verificar se o bFGF está envolvido na atividade
inibitória de GSE;– Produção de NO.
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MATERIAIS e MÉTODO
• Extrato de G. sinensis
• Cultura de linhagens cancerosas
• ELISA quantificação bFGF
• Quantificação NO (NO2-)
• Mudanças morfológicas
• Ensaio da Sulforadamina B
Go To
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Trituração Etanol Agitação e aquecimento
Secagem e mensuração da [ ]Diluição
Voltar
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DOAÇÃO
Condições de incubação: 37º C e 5 % CO2
10 %
Voltar
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Voltar
Coleta de meio e adição de GSE (10,
15, 20 µg/mL)
6/ 24 hProt. Totais (Bradford)
pg de bFGF/ 0.1
mg de prot.totais
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Tratamento com GSE
Coleta do meioSolução de
Greiss
(540nm)
µM NO2-
Nível de síntese de NO Voltar
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Adição de 20 µg/mL GSE
6h
Voltar
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Troca de meio e adição de
GSE
Ác. Tricloroacético
(fixador)
6 / 24 h
Sulforodamina B (corante)
CentrifugaçãoTampão Tris
540 nm Voltar
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RESULTADOS
• GSE e as culturas;
• Mudanças na morfologia celular;
• NO;
• ELISA (bFGF).Go To
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Voltar
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Voltar
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DISCUSSÃO
• Crescimento celular• bFGF ação às vezes contraditória• GSE bFGF inibição crescimento• GSE [NO2
-] inibição
• GSE induz inibição envolvendo nível bFGF– Indução produção NO
• GSE anti-angiogênico• Medicamento
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OBRIGADO !!!!!
DIOGO B. PROVETE
Grupo de Biologia Celular e TecidualGrupo de Biologia Celular e Tecidual
Laboratório de Microbiologia e ImunologiaLaboratório de Microbiologia e Imunologia
Departamento de Ciências Biológicas Departamento de Ciências Biológicas
Efoa/Ceufe – Alfenas-MGEfoa/Ceufe – Alfenas-MG
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