Instruct ion Sheet012-10624A

EcoZone SystemME-6668

800-772-8700 www.pasco.com

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Included Equipment

• 3 EcoChambers with lids

• Tray

• 3 grommets for coupling chambers

• 28 assorted stoppers for closing unused holes

• Cotton rope for wicking moisture between chambers, 900 cm

• Syringe and plastic tubing with connector for sampling water, replenishing water, and injecting pollutants

Recommended PASPORT Equipment

• Xplorer GLX (PASCO part PS-2002)

• Water Quality Sensor (PS-2169)

• Oxygen Gas Sensor (PS-2126)

• Carbon Dioxide Sensor (PS-2110)

• Temperature Sensor (PS-2125)

• Weather Sensor (PS-2154)

• Turbidity Sensor (PS-2122)

• Water Quality Colorimeter (PS-2179)

• ezSample Water Quality Testing Kits:

• Iron (EZ-2331)

• Sulfate (EZ-2332)

• Nitrate (EZ-2333)

• Ammonia (EZ-2334)

• Fluoride (EZ-2336)

• Phosphate (EZ-2337)

• Chlorine (EZ-2339)

Recommended ScienceWorkshop Equipment

• ScienceWorkshop 500, 700, or 750 Interface

• DataStudio

• Oxygen Gas Sensor (CI-6562)

• CO2 Sensor (CI-6561)

• Temperature Sensor (CI-6526)

• Colorimeter (CI-6747)

IntroductionThe PASCO ME-6668 EcoZones System is designed to help stu-dents model and understand the complex interactions within and among different ecosystems. The three clear acrylic EcoCham-bers are designed to accommodate PASCO sensors, making qual-itative and quantitative measurements as easy as visual observation.

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Students can measure many aspects of the traditional ecosys-tem—terrestrial, aquatic, and decomposition chambers. Students can also use each chamber individually by decoupling the cham-bers and constructing a single biome.

The unique design of the EcoZones allows the system to remain closed, which allows for measurements to have a minimal impact on the system. Environmental science probes can be used to actively measure variables in the ecosystem model. A syringe and tubing are included to extract water for chemical testing, replenished water without opening the system, and inject pollut-ants for chamber manipulation.

Set-up and Experiment ConsiderationsBefore setting up the EcoZone System, there are some things that should be taken into consideration.

Consider what you are interested in measuring. There are nine open spaces available for sensors in the lids of the chambers; however, the Xplorer GLX has only 4 ports. If you intend to mea-sure water quality (dissolved oxygen, conductivity, etc.), con-sider using the Water Quality Sensor, which supports several different probes, instead of individual sensors to save port space.

A cotton rope is included to allow for efficient wicking of water from the aquatic chamber to the other chambers. Be sure that this wick covers each chamber sufficiently. Lay the wick underneath the plants both under and over the plants in the terrestrial cham-ber, for example, to ensure that plants receive sufficient water. Use approximately 36 inches of rope.

Cotton rope is used for its excellent wicking ability; however note that cotton in contact with soil may decompose over a period of weeks. You may need to check and replace it periodically.

Equipment Set-upImportant: When carrying the EcoZone System, do NOT pick the system up by the aquatic chamber (the heaviest chamber). Pick the entire system up by the tray.

1. Place the three EcoChambers into the custom tray.

2. Connect the EcoChambers using the rubber grommets as illustrated.

3. Fill each EcoChamber according to the instructions in the lab you are performing.

4. Select the sensors and interface that you intend to use for the experiment.

5. Place the lids onto the EcoChambers, lining up open arches at the bottom of each lid with the rubber connection grom-mets on the chambers.

6. Insert large stoppers with holes into the holes in the lids.

7. Insert probes into the holes in the stoppers.

8. Insert small solid stoppers into unused holes in the larger stoppers.

9. Connect the sensors to Xplorer GLX or other interface.

Sensor CalibrationCalibrate sensors prior to using them in an experiment. Refer to the documentation provided with each sensor for calibration instructions.

Note: In some cases, sensors do not require calibration. If, for example, you wish to measure only relative dissolved oxygen changes, the sensor does not to be calibrated.

Xplorer GLX Set-upNote: The interface set-up and data collection instructions and sample data in this and the following sections apply to the Xplorer GLX datalog-ger. Other data collection systems can also be used.

EcoZones experiments are typically long-term experiments. If data is collected at the default rate, the volume of information may fill the memory of the GLX before the end of the experi-ment. To prevent the Xplorer GLX from ending the experiment early, change the sample rate of every sensor.

1. Press h+I to access the Sensors screen. The sensors currently connected to the Xplorer GLX will show across the top of the screen. The Sample Rate Unit will be highlighted for the sensor in port 1.

Chambers connected with grommets

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2. Press c to see the available sample rate units for that sen-sor. Highlight “minutes” using the arrow keys and press c.

3. Use the arrow keys to highlight Sample Rate and press c. Change the sample rate to 1, 2, or 5 minutes between sam-ples.

4. Use the arrow keys to highlight the sensor in port 2. Press c to select that sensor, and repeat steps 2 and 3 to change the sample rate. Do this for the remaining sensors.

Data Collection1. Ensure that the GLX is in Continuous data collection mode.

a. Press h+I to access the Sensors screen.

b. Press F to access the Mode menu. If necessary, use the arrow keys to select Continuous and press c.

2. With the sensors in place, press s to begin recording data.

3. Allow the experiment to run for the specified time. Do not bump or jar the EcoZones, as this may cause the sensors to record erratically.

4. Press s to stop collecting data.

Data AnalysisGraphs showing two variables changing over time are most valu-able in the EcoZones experiments because they show the rela-tionship between related quantities, such as oxygen and carbon dioxide concentrations, and how these relationships changes over time.

Example Activities• Construct a traditional ecosystem with a aquatic, terrestrial,

and decomposition chamber.

• Construct a single biome, such as a desert, trundra, or man-grove biome.

• Monitor the effects of light on the photosynthesis of a plant in a single chamber.

Sample Data

This graph shows how the concentrations of oxygen (black) and carbon dioxide (gray) change as a light is turned on and off.

This graph shows how the relationship between the relative humidity (black) and temperature (gray) within a terrestrial chamber over a

24-hour period.

Technical SupportFor assistance with any PASCO product, contact PASCO at:

For more information about the EcoZone System and the latest revision of this Instruction Sheet, visit:

www.pasco.com/go?ME-6668

Limited Warranty For a description of the product warranty, see the PASCO catalog. Copyright The PASCO scientific 012-10624A Eco-Zone System Instruction Sheet is copyrighted with all rights reserved. Per-mission is granted to non-profit educational institutions for reproduction of any part of this manual, providing the reproductions are used only in their laboratories and classrooms, and are not sold for profit. Reproduction under any other circumstances, without the written consent of PASCO sci-entific, is prohibited. Trademarks PASCO, PASCO scientific, DataStu-dio, ezSample, PASPORT, ScienceWorkshop, Xplorer, and Xplorer GLX trademarks or registered trademarks of PASCO scientific, in the United States and/or in other countries. All other brands, products, or service names are or may be trademarks or service marks of, and are used to identify, products or services of, their respective owners. For more infor-mation visit www.pasco.com/legal.

Address: PASCO scientific10101 Foothills Blvd.Roseville, CA 95747-7100

Phone: 916-786-3800 (worldwide)800-772-8700 (U.S.)

Fax: 916-786-7565

Web: www.pasco.com

Email: support@pasco.com