eau that smell poster rgb - biobuilder.org · eau that smell density under the hood isoamyl alcohol...
TRANSCRIPT
ATCG...
GROWTH PHASES
“LAG” “LOG” “STATIONARY”
TIME
“LAG” “LOG” “STATIONARY”
CELLDENSITY
EAU
TH
AT
SMEL
L
UNDER THE HOOD
Isoamyl alcohol will be added to the media as input for the banana-smell generating device
THE SYSTEMTHE PREPARATION
CH3
H3C OH
A gene from S. cerevisiae converts isoamyl alcohol into isoamyl acetate, which smells like bananas.
BGD”STATIONARY” phase promoter
BGD”LOG” phase promoter
POSITIVE CONTROL DESIGN 1 DESIGN 2 NEGATIVE CONTROL
“LAG” “LOG” “STATIONARY”
”STATIONARY” phase promoter
INVERTER BGD
“LAG” “LOG” “STATIONARY”
Indole deficientE.coli only
S. cerevisiaeYEASTalcohol acetyltransferase
geneINDOLE DEFICIENT
E.coli
Indole is naturally produced by E.coli. The Eau dʼcoli strain was modified to eliminate indole production and its stinky smell.
ATF1
BANANA-SMELL GENERATING DEVICE