early cmos development project 2005-2008
TRANSCRIPT
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Early CMOS development project 2005-2008
• LMB, FEI, MPI-Biophysics and MPI-Biochemistry sponsored a pre-commercial project
with RAL named TEMAPS.
• Goal was to maximise DQE for 300keV electrons in an integrating detector, and to make it
more-or-less a straight replacement for film and phosphor/CCD detectors.
• Many parameters were optimised.
FEI Falcon CMOS direct electron detector is a product
• Use same beam intensity, same exposure time as for SO-163 (12 in D19).
• e.g. 1 sec exposure at 59,000x, with exposure meter set to 1 sec (emulsion 1). This gives
17el/Å2/sec at specimen, 17 Hz frame rate, 1.71 Å pixel, 3 el/frame/pixel, 50 el/pixel/sec.
• Vary dose by varying exposure time or magnification (keep same intensity at detector).
• To do better than Falcon in integrating mode, must go to counting mode.
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DQE() = DQE(0)*MTF2/NTF2
Meyer & Kirkland (2000)
De Ruijter (1995)
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(Pre-Falcon)
McMullan et al (2009a, 2009b, 2009c) Ultramicroscopy
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From FEI Falcon brochure
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LMB Falcon mounting and single frame acquisition system – Greg McMullan
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LMB report on backthinned Falcon May 2011
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Best region, area of 280x280 pixels
single frame sum of 26,000 frames
Single electron event processed images – backthinned Falcon
Electron counting with Falcon
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Backthinned Falcon - 1000 mesh grid shadow pattern – single electron comparison
integrating mode centre-of-gravity centre-of-gravity interpolated
Integrating mode Counting mode “Super-resolution” mode
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0 0.5 1 1.5 2Fraction of Nyquist
0
0.2
0.4
0.6
0.8
1
DQ
Es4 MTF
2
Integrating
Backthinned Falcon -300 keV
s4
s2
theoretical
s1
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T12/SO-163 Krios/Eagle Polara/BT-Falcon
120kV 80kV 300kV
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Acknowledgements
• Wasi Faruqi MRC-LMB
• Greg McMullan MRC-LMB
• Shaoxia Chen MRC-LMB
• Renato Turchetta RAL/STFC
• Nicola Guerrini RAL/STFC
• Gerald van Hoften FEI