Culture Media

OXOID DRY-BAGS PROVIDE A QUICK AND CONVENIENT SOLUTION FOR FOOD TESTING

LABORATORIES. AVAILABLE WITH SINGLE-USE FILTERS FOR MAXIMUM CONVENIENCE

OR WITHOUT FOR A MORE ECONOMICAL OPTION. AUTOCLAVABLE FILTERS FOR USE

WITH DRY-BAGS ARE ALSO AVAILABLE.

Dry-Bags

▼ O P E N ▼

▼ F I L L ▼

D I S P E N S E

EASY TO USESimple to fill and dispense – easy-to-follow instructions provided.

SPACE-SAVINGConveniently packed in boxes of five or ten, Dry-Bags take up relatively little spacecompared to prepared media and can be made up as required.

QUALITY GUARANTEEDQuality certificates available for every batch to help reduce the burden of QC testing.

CONVENIENTQuick and easy to prepare – just add water to each Dry-Bag and they are ready to usein minutes.

SAFENo handling of glassware or weighing of culture media required. Empty bags can bedisposed of with normal laboratory waste.

COST EFFECTIVEDry-Bags are light to transport. There is no labour-intensive production or sterilisationrequired, reducing the burden on media preparation departments and equipment.

Procedure for Connecting, Dispensing, Disconnecting and Re-connecting Oxo

1 Remove the Dry-Bagfrom the outer packaging;place onto a flat surfaceor trolley, and shake todistribute the dehydratedpowder.

2 Ensure that the clips onboth tubes of the Dry-Bagremain in the closed position throughout theconnecting process. If using Dry-Bags withoutfilters go to Step 3a. If using standard Dry-Bags go to Step 3b.

3b Connect pump tubingto the filter on theDry-Bag and secureusing one of theTube-Ties provided.

4 When ready, open theclip between the Dry-Bagand the filter. It may benecessary to squeeze thetubing to fully open it.

7 Whilst filling, manually agitate the bag until the powder is completely dissolved. Once the required volume of water has been added (i.e. 20litres), turn off the water supply and close the clip between the filter and bag. If using an Oxoid Dry-Bag Filter (order code DB0200A) it may nowbe removed. If the disconnection is carried out aseptically the Dry-Bag Filter may be used to fill further bags (up to a total volume of 200 litres).Alternatively, the Dry-Bag Filter may be sterilised by autoclaving at 121ºC for 15 minutes between each use. The filter may be autoclavedand reused up to 10 times.

10 Once ready to dispense,the clip on the outlettube may be opened (itmay be necessary tosqueeze the tube to fullyopen it). A pump orautomatic diluter shouldbe used to dispense themedium into the finalrecepticle.

11Begin dispensing. Tilt the Dry-Bag to remove the lastof the liquid. If required, unused or partially used,bags may be stored for up to 72 hours at 4-25°C.Ensure sterility of the outlet tube is maintained at alltimes during use and storage.

Connecting and Dispensing the Dry-Bag

oid Dry-Bags

5 Begin to fill the bagusing a peristaltic pumpor other suitable equipment. Ensure thatthe flow of water intothe bag does not exceed2 litres/minute as thismay damage the filter.

3a Remove the cap from the end of one of the Dry-Bagtubes. Aseptically insert a sterile Dry-Bag Filter (ordercode DB0200A) - ensure the filter is correctly orientatedi.e. with the black arrow pointing towards the bag.Secure the Dry-Bag tubing using one of the Tube-Tiesprovided. Continue to step 3b.

6 As the water flowbegins, ensure thereare no leaks around thefilter connections. If atany time during filling,air builds up around the filter, a block may occur- any air can be released by quickly opening and re-closing the vent on the side of the filter.

9 Remove the cap fromthe outlet tube andasceptically insert asterile Bag Connector.Ensure that it is fullyinserted.

12Dispose of the empty Dry-Bag with normal laboratory waste. The stainlesssteel Bag Connector may be cleaned andsterilised for re-use.

8 A Bag Connector (order codeBM9901A) and dispensing tubeset should be pre-sterilisedand ready to connect to theDry-Bag outlet tube (unusedtube). NB. If it is anticipatedthat the Dry-Bag will bedisconnected before emptying,slide a second clip onto theoutlet tube before inserting theBag Connector. (See over pagefor disconnection instructions).

For More Information

To find out more about the Oxoid Dry-Bag range of products, please speak to your local Oxoid representative or contact us using the detailsbelow.

D E D I C AT E D T O M I C R O B I O L O G Y

www.oxoid.comOxoid, Wade Road, Basingstoke, Hants, RG24 8PW, UK. Tel: +44 (0) 1256 841144 Fax: +44 (0) 1256 329728 Email: oxoid@thermofisher.com

1 Before disconnecting theBag Connector, close theclip provided on the outlet tube and then usea second clip to seal thetube a short distancealong from the first clip.

1 Leave the clips on theoutlet tube in the closedposition.

2 Using sterile scissors cutthe outlet tube as shown.

3 Keep the remaining clipon the outlet tube in theclosed position andaseptically connect theoutlet tube to a new,sterile Bag Connector/tube set. Ensure the BagConnector is fully insertedto form a tight seal.

4 The clip on the outlettube may now be openedto enable dispensing tocommence. The cut, endpiece of tubing should be discarded.

2 The bag can now be disconnected and theprepared medium storedat 4-25°C for up to 72hours from the time itwas made up.

Disconnecting the Dry-Bag for Re-use

Re-connecting the Dry-Bag

Folio: 1152/07/07

© 2007, Oxoid Ltd.; copyrights to photographs held separately; contact Oxoid Ltd. for details. Photographs may not be extracted or reproduced in any way.