dr. mueller’s mechanistic enzymology lab chemistry and biochemistry department
DESCRIPTION
A Day in the Lab with. Name: Ms. Marguerite McDonald Title: Biochemist, grad student Concentration: Enzymology Work Place :. Dr. Mueller’s Mechanistic Enzymology Lab Chemistry and Biochemistry Department University of Delaware Graduate Program. PROTEIN. RNA. DNA. The Central Dogma. - PowerPoint PPT PresentationTRANSCRIPT
Name: Ms. Marguerite McDonald Title: Biochemist, grad studentConcentration: EnzymologyWork Place :
Dr. Mueller’s
Mechanistic Enzymology Lab
Chemistry and Biochemistry Department
University of Delaware Graduate Program
Biochemistry
DNA RNA PROTEIN
http://bioweb.wku.edu/courses/BIOL115/Wyatt/Nucleic_Acids/mRNA2.htm
Mechanistic EnzymologyThe study of the action (the mechanism) of an enzyme
http://www.sdnhm.org/exhibits/epidemic/teachers/images/bacteria.jpg
Pseudouridine synthases
synthase
synthase
The Chemistry: Isomerization of RNA
TruA’s substrate: transfer RNA
Purifying radioactive tRNA and TruA
urea-polyacrylamide gel showing pure tRNA and tRNA with DNA contamination
SDS-polyacrylamide gel showing pure TruA and TruA before purification
HPLC trace and radioactivity/ min
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time
dpm
HPLC tracedpms/10 ul fraction
Pseudouridine Synthases