dr. carlo ventura - dna and cell reprogramming
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Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
DNA and Cell Reprogramming via Epigenetic Information Delivered by Magnetic Fields, Sound Vibrations,
and Coherent Water
Carlo Ventura, MD, Ph.D.Full Professor of Molecular Biology
University of Bologna, Italy
VID (Visual Institute of Developmental Sciences) Laboratory of Molecular Biology and Stem Cell Engineering, NIBB , Bologna, Italy
Experimental Cardiology at the Cardiovascular Department, University of Bologna, Italy
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
This cellcan form the
Embryo and placenta
This cellcan just form
the embryo
Fully mature
Toti-potent
Pluri-potent
Multi-potent
Stem Cells and Regenerative Medicine
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
NOT ONLY MOLECULES!BUT…
Turning on stem cell cardio-genesis with extremely low frequency magnetic fields
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Turning on Stem Cell Cardiogenesis with Extremely Low Frequency Magnetic Fields
Ventura C et al. FASEB J 2004
Sinusoidal Magnetic Field (MF)(50 Hz, 0.8 mTrms)
Genes that Trigger Cardiac Cell Commitment Increases Cardiac Specific Genes and Proteins
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Dynorphin B (Natural Chemistry) Magnetic Fields (Magnetic Waves)
Spontaneously Beating Cardiomyocytes Appearing After Treatment of Mouse Embryonic Stem Cells with:
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Magnetic fields in the Wi-Fi band of 2.4 GHz, the same used with wireless Internet connections worldwide, were used with an innovative device, called the Radio Electric Asymmetric Conveyer (REAC), to modulate the ability of stem cells to differentiate or even make adult non-stem cells behave like stem cells.
The REAC technology is based upon the interaction of two oscillating magnetic fields. One is generated by cells or the entire organism, and the other is a weaker electromagnetic field produced by the REAC system. A resultant electromagnetic field induces the cells to respond with endogenous self-generated micro-currents, likely ensuing from cellular ionic fluxes. These currents are detected and conveyed back to the cells with a conveyer probe.
Magnetic Energy: A Tool for Modulation of Cell Signaling and Fate
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
REAC - Modulated Magnetic FieldsDrs. Salvatore Rinaldi and Vania Fontani
Radiofrequency generators and irradiating antennas (1) create a 2.4 GHz radiofrequency field; electrodes (2) connected through a cable (CV). The radio frequency field induces radiofrequency currents in the body, which are detected and conveyed back by the electrodes.
The REAC apparatus adapted to an embryonic stem cell culture system within a CO2 incubator.
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Distance between the RF emitter and the culture medium is approximately 35 cm.Electromagnetic quantities (detected by receiving antenna and spectrum analyser; single radiofrequency bursts of 250 ms. with an off interval of 2.5 seconds):
• Radiated power: 2 mW,• Electric field: 0.4 V/m,• Magnetic field: 1mA/m,• Specific Absorption Rate – SAR: 0.128W/g,• Density of radio electric current flowing in the culture medium during the REAC
single radiofrequency burst (=1 A/V.m and =1000Kg/m3), J=30A/cm2.• Specific power: around 400 μW/m²
EXPOSURE CONDITIONS
With REAC Without REAC
Embryod Bodies2 days in suspension
Undifferentiated Cells
Additional 7 days in tissue culture
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DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Radiofrequency Energy Loop Primes Cardiac, Neuronal, and Skeletal Muscle Differentiation in Mouse Embryonic Stem Cells:A New Tool for Improving Tissue Regeneration
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DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Modulated Magnetic Fields Primes the Expression of Genes Driving Cardiac, Neuronal and Skeletal Muscle Differentiation
Cardiac Driving Genes
Muscle Gene Neuron Gene
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DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Embryonic stem cell exposure to modulated magnetic fields affects the expression of stem cell related proteins and optimizes expression of pluripotent cells.
Cardiac Proteins Neural Proteins
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DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Control REAC
Modulated magnetic fields enhances the yield of embryonic stem cell derived cardio-myocytes and affects the quality of beating and contractile dynamics.
REAC Treated
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DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Human Adult Stem Cells Were Exposed to REAC-Modulated Magnetic Fields
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Fat tissue aspirates are processed in a non-enzymatic fashion through a new technology (Lipogems) to harvest stem cells.
Filling with PBSl
Filling with
lipoaspiratel
Washing/Emulsion
Expulsion of Lipogem product
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Morphological Appearance of Stem Cells
Day 3 Day 4
Day 6 Day 12
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Gene Expression Analysis in Stem Cells Exposed to Modulated Magnetic Fields
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Modulated Magnetic Field Exposure Triggers Expression of Cardiogenic Genes
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Modulated Magnetic Field Exposure Triggers Genes Essential for Neurogenesis and Skeletal Myogenesis
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Confocal Microscopy Analysis of the Effect of Modulated Magnetic Fields on the Expression of Selected
Marker Proteins in Human Adult (Adipose-Derived) Stem Cells
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Magnetic Fileds Drive Human (Adipose-Derived) Stem Cell Differentiation
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
The 2012 Nobel Prize in Physiology or Medicine: for the discovery that mature cells can be reprogrammed to become pluripotent.
The New York TimesBy Nicolas WadeTwo scientists who were awarded the Nobel Prize in Physiology or Medicine on Monday helped lay the foundation for regenerative medicine, the hotly pursued though still distant idea of rebuilding the body with tissues generated from its own cells. They are John B. Gurdon of the University of Cambridge in England and Shinya Yamanaka of Kyoto University in Japan.Both men had to overcome false starts in life. Dr. Gurdon was told as a boy that he was wholly unsuited for biology, and Dr. Yamanaka trained as a surgeon only to find he was not so good at it.
Dr. Yamanaka Dr. Gurdon
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Modulated Magnetic Field Exposure Does Not Affect Cell Viability
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Mef2c Tbx5 Gene
GATA4 Gene
Modulated Magnetic Fields Can Drive Human Fibroblasts into Cardiac Gene Reprogramming
Mef2c Gene
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DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Modulated Magnetic Fields elicits the expression of genes orchestrating human fibroblast commitment towards cardiogenic,
skeletal myogenic and neurogenic lineages.Prodynorphin Gene Nkx2.5 Gene
Neurogenin1 Gene MyoD Gene
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
cMyc GeneSox2 Gene Oct4 Gene
Nanog Gene Klf4 Gene
Modulated magnetic field treatment time-dependently modulates the expression of genes involved in fibroblast
reprogramming into pluripotent cells.
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Cardiac Cells Skeletal Muscle Cells Neural Cells
Modulated Magnetic Fields Primes Human Fibroblast Differentiation
into Lineages in which Cells Would Never Otherwise Appear
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Cell Reprogramming with
Sound Vibration
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Extracellular and Intracellular Environments:A World of Continuous Vibrations
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Atomic Force Microscopy to Detect Cellular Nano-mechanical Vibrational Patterns
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
22 °C
26 °C
30 °C
Dead cell
Atomic Force Microscopy of Nano-mechanical Signatures During Stem Cell Development
“Nano-mechanical characterization of cellular activity – Sonocytology”
Carlo Ventura (NIBB-VID) & James K. Gimzewski (UCLA)
With Mike Horton–UCLA, Mark Welland and members of Dept. of Chemistry, Terry McMaster-Bristol, Daniel Roberts-Bristol,
Henrich Horber–UCLA, Quentin Pankhurst–UCLA, Andrew Fisher–UCLA
Sound from single yeast cell
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Atomic Force Microscopy and Nano-mechanical Probing:Transcription Factor Dynamics of DNA
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
General Transcriptional Machinery
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Water can be “Signalized” Non-Locally with Biological Information to Generate Coherent Domains Capable of
Quantum Signaling and Information Transfer
Developments in Implicit Computational Complexity
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Crucial Experimental Details:•Filtration: 450/100 nm for bacterial DNA, 450/20 nm for viral DNA.•High dilutions in water.•Mechanical agitation (vortex) between each dilution.•Excitation by the electromagnetic background of extremely low frequency, starting at 7 Hz. •The excitation is not induced when the system is shielded by a mu-metal cage.
Typical signals from aqueous dilutions of M. Pyrum. Positive signals from D-7 to D-12 dilutions.
Device for the capture and analysis of EM signals1.Coil made up of copper wire, impedance 300 Ohms.2.Plastic tube containing 1ml of the solution to be analyzed. 3.Amplifier. The solution contained viral or bacterial DNA Fragments.
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
LTR HIV DNA Fragment
Transmission of DNA Information into Water Through Electromagnetic Waves
DNA with 98% sequence identical to the original DNA sequence.
Water nanostructures and their electromagnetic resonance can faithfully maintain DNA information.
Nucleotides, primers, polymerase amplification
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
Information is thought to be the essence of life, as in the DNA code. We have growing needs of separating the “informational content” of life
from its “material substrate.”
In both the philosophical and visual sense, ‘seeing is believing’ does not apply to nano-technology, for there is nothing even remotely visible. On the atomic and molecular scale, data is recorded by sensing and probing in a very abstract manner, which requires complex and approximate interpretations. More than in any other science, visualization and creation of a narrative becomes necessary to describe what is sensed, not seen.
Connecting Hearts and Minds
DNA and Cell Reprogramming via Epigenetic Information ©2013 Visual Institute of Developmental Science
NIBB - VID Laboratory of Molecular Biology and Stem Cell Engineering Bologna, ItalyCarlo Ventura Silvia Cantoni Francesca Bianchi Claudia CavalliniMargherita Galletti Riccardo TassinariElena OliviIrene Frascari
Rinaldi-Fontani Institute Florence, ItalySalvatore RinaldiVania FontaniAlessandro CastagnaI
Department of Biomedical Sciences, University of Sassari, ItalyMargherita MaioliSara SantanielloGianfranco PigliaruSara Gualini
NFN, ItalyEmilio Del GiudiceAlberto TedeschiGiuseppe Vitiello
Department of Chemistry and BiochemistryUCLA, LA, USAJames K. GimzewskiSarah Cross
Institute of HeartMathBoulder Creek, CA, USARollin McCraty
Prince Sultan Cardiac CenterHofuf/AlahsaKingdom of Saudi ArabiaAbdullah Abdulrhman Alabdulgader
Cell Transplant CenterDiabetes Research Institute (DRI) WorldWide DRI FederationMiller School of MedicineUniversity of Miami, USACamillo RicordiCarlo Tremolada
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