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The wacky history of the cell theory
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Microscopy • Janssen’s microscope (1590’s)
capable of magnifying images approximately three times when fully closed and up to ten times when extended to the maximum.
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Van Leeuwenhoek
• “The Father of Microbiology”
• first person to observe and describe single celled organisms (microorganisms)
• first to record and observe muscle fibers, bacteria, spermatozoa and blood flow in capillaries (small blood vessels).
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Robert Hooke (1665)
Coined the term “cell”
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Eyes on your notes packet!
• our compound microscopes are called light microscopes
• parts of microscope (diagram sheet) and their functions
• lenses:
• 1. eyepiece lens: 10x
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2. objective lenses:
• very low: 5x (total=50X)
• Low: 10x (total= 100X)
• high: 43x (total=430X)
• oil: 97x (total=970X)
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• light microscopes limited to 2000x
• why? The wave length of light only allows us to see object at 2000X or less
• resolution drops with increase in magnification
• resolution= the ability to see detail in a in/on an object under the microsope
• unit of measure = μ micron (SI unit of length measurement) = 0.000001m
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TEM’s: Transmission Electron Microscopes
• use electrons in place of light
• focus with magnets
• electron beam passes through thin section of object (therefore not alive)
• magnifies 200,000x: photo enlarged 5x: total= 1,000,000X
• (no resolution problems)
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Transmission Electron Microscope (TEM)
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SEM’s: Scanning Electron
Microscopes
•shows surface of object in 3D
•allows very high magnification
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Scanning electron microscopes (SEM)
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Match the image with the type of microscope that produces it
Light microscope
Scanning electron microscope
Transmission electron microscope
A B C