Download - TECHNICAL PROTOCOL UNDER MKSP-NTFPmksptasar.in/adminpradanmkc/reports/124_technical_protocol_.pdf · Mahila Kisan Sashaktikaran Pariyojana (MKSP) Protocol- 1 TECHNICAL PROTOCOL UNDER

TECHNICAL PROTOCOL UNDER MKSP-NTFP

Promotion of Large Scale Tasar Sericulture Based Livelihood

Under

Mahila Kisan Sashaktikaran Pariyojana (MKSP)

Protocol- 1

TECHNICAL PROTOCOL UNDER MKSP-NTFP Tasar Projects

SUBJECT: NURSERY RAISING

Name of the Coordinating/ Technical Support Agency:

Central Silk Board, Ministry of Textiles, Govt. of India

Names of the Project Implementing Agencies:

Professional Assistance for Development Action (PRADAN)

Bharatiya Agro Industries Foundation (BAIF)

Society for Elimination of Rural Poverty (SERP) with Kovel Foundation as its FIA

Training module developed (write Yes/ No): Yes

Major activities Practices (Please mention the name of specific

practice/method/substance/equipment used)

Variety Arjun or Asan (from healthy trees)

Source of Seed Own (collected from selected healthy trees of 15-20 years age)

Seed selection � Should be healthy and dry.

� Should not have broken coat � Weight should not be less than 2 gram

� Free from insect attack

Seed requirement � Seed requirement to raise 15,000 seedling is 120 kg (6 bags)

Seed soaking � Sorting seed and filling especially in a gunny bags

� Gunny bag should be filled 70% of its capacity � Bag should be dipped in a water for 96 hrs for Arjuna seeds and 48 hrs

for Asan seeds

� Seed soaking will start when 40% poly bags filling is over � Seed soaking schedule:

� 1st day: : 2 bags � 4th Day: : 2bags

� 7th Day: : 1bag

� 1 bag of seed should be kept in stock that can be used in case of failure of germination or shortage of sprouted seedlings

Germination of seed � Before lifting the seed from water a 3" thick sand bed of size 5' or any suitable length x 3' should be prepared for sprouting

� Sand bed should be prepared in a shady place

� The soaked seed should be spread on the sand bed in two layers and the bed has to be covered with paddy straw and gunny bags

� Sprinkle water regularly over it to keep the bedcover moist Sprouting of seed will start on the 8th or 9th day and continue for three

weeks.

Rooting media preparation � First, soil should be crushed and screened on the wooden cot or iron

mesh. � A mixture of soil, sand and vermicompost (soil: 800 gm, sand: 400 gm

and vermicompost: 100 gm) should be mixed thoroughly

� Rooting media should be filled in a polythene bag of 9”x5” size, tightly leaving 1" vacant from the top to hold water.

� Filled polythene bags should be kept vertically in a nursery bed very closely in a compact form

Seed treatment � Collecting germinated seed and treating with fungicide Bavistin 2gm/lt

or Diathane M 45 2gm/lt or bio-fungicide through dipping.

Dibbling � Watering of rooting media continuously for 3 days in the morning and

evening so that the poly bags are completely drenched. Small portions

of dry rooting media at the bottom of a tube can create problems after dibbling

� Germinated seed should be placed in poly bags just after sprouting which starts 8 days after heap making and completed within 12-14 days

from seed soaking

� Sprouted seed should be collected in a basket from the sand bed and should be dipped in a fungicide solution before dibbling

� Dibbling should be strictly carried out in a completely drenched poly bags and in the evening only. No dibbling should be done in the

morning



� Dibbling should be done vertically without leaving intermittent space below the radical to avoid wilting

� Radical of the seed should be kept vertically below and soil should be tightly placed all round the seed to protect the radical from hot air.

� Before dibbling poly bags should be drenched and from next day regular watering should be done in the morning (before 7 AM) and in the

evening just before sunset.

� Shade should be provided to the bed at a height of 1 ft. The shade should be removed in the evening time before watering and should be

placed again on the bed after watering in the morning time

Nursery protection

measures

� Dimethoate (Rogar) spray after 30 days of dibbling to prevent seedling from insect attack (dose:1 ml/ltr of water)

� Fungicide should be sprayed if there is a fungal attack in a collar region of sapling.

Intercultural operations Fortnightly weeding should be practiced

Soil fertility mgmt. /Soil

health enrichment and crop nutrient management

� Urea spray after 30 days (dose: 5 gm/liter of water)

Bio-fertilizer/ Organic

Manure/ Green manure crops etc

� 100gm vermicompost is used per polythene bag as part of the rooting

media

Micro nutrient management � Dhanzyme (dose: 1 ml/liter of water) and planted (dose: 1.5 ml/liter of

water) spray after 45 days if the growth of seedling is not up to the mark. Enzymes should be sprayed after weeding, sorting and grading of

seedlings in nursery bed � Fungicide should be sprayed after 3 days of Dhanzyme spray.

Protocol- 2


SUBJECT: TASAR SILKWORM REARING by SEED AND COMMERCIAL REARERS










Tasar Silkworm Rearing Traditionally Tasar silkworm rearing conducted on naturally grown

trees of Asan and Arjuna. In order to increase productivity and better

management of rearing, food plants are also raised as block or economic plantation

Cleaning, disinfection & Hygiene of Rearing Field

� After pruning and leaf sprouting, Dimethoate (0.02%) should be sprayed in two doses with 15 days interval for gall control

� 0.5% and 2% bleaching powder solution should be sprayed on

the plants and soil respectively. � Before rearing, mixture of lime and bleaching powder (ratio 9:1)

should be dusted in the field.

DFL’s Procurement

Variety � Dhaba (BV) / Daba (TV)

Source of Seed � Private Graineur/ Basic Seed Multiplication &Training Centre

(BSM&TC)

Preparation for rearing � Selection of brushing site away from waterlogged and in well

ventilated area � To maintain chawki garden portion at an height of 6 to 8’ and

erection of nylon net � The ground area in and around rearing site should be cleared of

bushes and weeds, 15 days before brushing � Disinfection of rearing field and trunk of host plants with spray of

2% bleaching powder solution, 10 days prior to brushing � Dusting of slaked lime and bleaching powder mixture in ratio of

9:1 in the rearing field, 5 days prior to brushing

Transportation of eggs � Should be collected one day before hatching. � Transport DFL’s during cooler hours of the day or early/ late

hours of day to avoid any damage due to direct sunlight and high

temperature. � If collected 2-3 days before hatching time, incubate DFLs

properly.

Major activities Practices (Please mention the name of specific practice/method/substance/equipment used)

Incubation of eggs � Maintain 70-80 % Relative humidity & 280c Temperature with

minimum fluctuation � Use indigenous devices like earthen pitcher covered with wet

gunny cloth to incubate eggs � Incubate eggs for the period of 1 to 2 days � Take care of attack of lizards, birds and rats during incubation.

Keep away from smoke and fire � Conduct Black boxing of eggs for uniform hatching

Hatching of DFLs and brushing � Hatching should be done under shade on a blue polythene sheet � Hatched larvae should be fed with tender twigs and crawled over

worms transferred with help of soft brush/ feather



Chawki (Young-age) Rearing � Brushed worms along with tender twigs should be shifted to

chawki garden under nylon net � Rear chawki worms under nylon nets till 2nd moult � Foliar spray of Jeevan Sudha once in each stage only on the

branches where larvae are feeding � Foliar spray of Leaf Surface Microbes once during 2nd stage � Dusting of slaked lime and bleaching powder mixture in ratio of

97:3 once in 2nd stage

Late Age Rearing

Pre-preparation � Select well grown bushes or food plants for rearing larvae from III instar onwards.

� Clean and field disinfect rearing site before transferring chawki worms to food plants.

� Remove newly sprouted leaves during late age rearing. � Dusting of slaked lime and bleaching powder mixture in ratio of

97:3 once in 3rd, 4th and three times during 5th stage at an

interval of four days � Remove dead/ diseased worms to avoid secondary infection of

diseases

Care during moulting � Larvae should not be disturbed during moulting � The transfer should be done only 2-4 hours after ecdysis when

the worms are fed adequately.

After rearing � Disinfection of rearing field with spray of 2% bleaching powder

solution after one week after harvest of cocoons

Protocol- 3


SUBJECT: DISEASE MONITORING










Disease monitoring � To examine measures taken for field disinfection, disease

containing measures prior to and during rearing, at the time of

seed cocoon procurement, preservation and processing. � To assess and evaluate the quality of seed cocoon stocks

preserved at Basic Seed Production Units (BSPU)/ BSM&TCs. � To evaluate the extent of pebrine in the seed stock through

microscopic examination by adopting recommended methods and recommend the fitness of seed cocoons for nucleus/basic

seed production.

� To evaluate the method of seed cocoon preservation at different seed production units and suggest corrective measures, if any.

� To assess the availability of equipments and the microscopic examination facilities as well as the proper disposal system for

grainage refuse.

� To suggest steps to be taken for disinfection and other disease control measures prior to and during rearings for improvement in

seed cocoon stocks.

Schedule of examination � 15 days prior to brushing

� Larval examination during IV/V age

� Examination of pre-pupae prior to seed cocoon procurement and pupae, before preservation

� Moth examination during erratic emergence & grainage operation

Record of disease incidence � Details of disease incidence and its reporting need to be recorded in the prescribed format enclosed at the end.

Schedule of activities to be followed for disease monitoring in the field

Prior to brushing

15days before brushing The ground area in and around rearing site should be cleared of

bushes and weeds

10 days prior to brushing

Disinfection of rearing field and trunk of host plants with spray of

2% bleaching powder solution


Dusting of slaked lime and bleaching powder mixture in ratio of 9:1

in the rearing field



During rearing

Once each in 1st, 2nd and 3rd instar Foliar spray of Jeevan Sudha only on the branches where larvae are

feeding

Once during 2nd stage Foliar spray of Leaf Surface Microbes

Once each in 2nd, 3rd, 4th instar and

thrice during 5th instar at an interval of

4 days


After rearing



One week after harvest of cocoons Disinfection of rearing field with spray of 2% bleaching powder

solution

Prior to procurement of cocoons in the grainage

10 days before consignment of seed

cocoons

The grainage house and appliances should be washed thoroughly

with water next day followed by 3% formalin/ 2% Bleaching powder

solution.

6 days before consigning the seed

cocoons

Fumigation of grainage house with sulphur and formaldehyde

solution (1kg : 2lt)

2 days before consigning the seed cocoons

Open the doors and windows to remove fumes

1 day before consigning the seed cocoons

Sprinkling of slaked lime and bleaching powder (9:1) in and all

around the grainage house

During consignment of the cocoons

Every Day Clean the floor with bleaching powder solution and running water

During grainage operations

Every day � Clean floor and verandah with 5% bleaching powder solution

after completion of each day’s grainage operation � Use gunny cloth soaked in 5% formalin as foot mat tray at

grainage door

Every day � Sprinkle Slaked lime and bleaching powder (9:1) all round the grainage house

� Washing and surface sterilization of eggs

Disposal of grainage waste

Daily after mother moth examination Disposal of cut moths & rejected egg layings in soak pit and sprinkle bleaching powder

(a) Draft format for the Disease monitoring team during the grainage operation:

Name of

the BSPU/

BSM&TC

Source of

Seed cocoons

No. of

cocoons preserved

No. of pupae

tested

Pebrine % Other

disease incidence

if any.,

Remarks

Male Female Male Female

Note: Action suggested by the Team during their earlier visit and action taken by the unit need to be recorded.

Protocol- 4


SUBJECT: SILKWORM SEED PRODUCTION










Disease monitoring � To examine measures taken for field disinfection, disease

containing measures prior to and during rearing, at the time of

seed cocoon procurement, preservation and processing. � To assess and evaluate the quality of seed cocoon stocks

preserved at Basic Seed Production Units (BSPU)/ BSM&TCs. � To evaluate the extent of pebrine in the seed stock through

microscopic examination by adopting recommended methods and recommend the fitness of seed cocoons for nucleus/basic

seed production.

� To evaluate the method of seed cocoon preservation at different seed production units and suggest corrective measures, if any.

� To assess the availability of equipments and the microscopic examination facilities as well as the proper disposal system for

grainage refuse.

� To suggest steps to be taken for disinfection and other disease control measures prior to and during rearing for improvement in

seed cocoon stocks.

Schedule of examination � 15 days prior to brushing

� Larval examination during IV/V age

� Examination of pre-pupae prior to seed cocoon procurement and pupae, before preservation

� Moth examination during erratic emergence & grainage operation

Record of disease incidence � Details of disease incidence and its reporting need to be recorded in the prescribed format enclosed at the end.

Schedule of activities to be

followed for disease monitoring in

the field

Prior to brushing

15days before brushing The ground area in and around rearing site should be cleared of

bushes and weeds


Disinfection of rearing field and trunk of host plants with spray of

2% bleaching powder solution


Dusting of slaked lime and bleaching powder mixture in ratio of 9:1 in the rearing field

Major activities Practices (Please mention the name of specific practice/method/substance/equipment used)

During rearing

Once each in 1st, 2nd and 3rd instar Foliar spray of Jeevan Sudha only on the branches where larvae are

feeding

Once during 2nd stage Foliar spray of Leaf Surface Microbes

Once each in 2nd, 3rd, 4th instar and

thrice during 5th instar at an interval of

4 days


After rearing

One week after harvest of cocoons Disinfection of rearing field with spray of 2% bleaching powder

solution



Prior to procurement of cocoons

in the grainage

10 days before consignment of seed

cocoons

The grainage house and appliances should be washed thoroughly

with water next day followed by 3% formalin/ 2% Bleaching powder

solution.

6 days before consigning the seed

cocoons

Fumigation of grainage house with sulphur and formaldehyde

solution (1kg : 2lt)

2 days before consigning the seed cocoons

Open the doors and windows to remove fumes

1 day before consigning the seed cocoons

Sprinkling of slaked lime and bleaching powder (9:1) in and all

around the grainage house

During consignment of the

cocoons

Every Day Clean the floor with bleaching powder solution and running water

During grainage operations

Every day � Clean floor and verandah with 5% bleaching powder solution

after completion of each day’s grainage operation � Use gunny cloth soaked in 5% formalin as foot mat tray at

grainage door

Every day � Sprinkle Slaked lime and bleaching powder (9:1) all round the

grainage house

� Washing and surface sterilization of eggs

Disposal of grainage waste

Daily after mother moth examination Disposal of cut moths & rejected egg layings in soak pit and sprinkle

bleaching powder

(b) Draft format for the Disease monitoring team during the grainage operation:

Name of the

BSPU/ BSM&TC

Source of Seed

cocoons

No. of cocoons

preserved

No. of pupae tested

Pebrine % Other incidence

of disease if any.

Remarks

Male Female Male Female

Note: Action suggested by the Team during their earlier visit and action taken by the unit

need to be recorded.

Download - TECHNICAL PROTOCOL UNDER MKSP-NTFPmksptasar.in/adminpradanmkc/reports/124_technical_protocol_.pdf · Mahila Kisan Sashaktikaran Pariyojana (MKSP) Protocol- 1 TECHNICAL PROTOCOL UNDER

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