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T. Trimpe 2005 http://sciencespot.net/
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Base
-Single piece of metal-Support here when moving
Color RED
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Arm
-Hold here when moving
Color Dark GREEN
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Stage
-Supports Specimen-Hole allows light to pass through
Color BLUE
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Diaphragm
-controls amount of light-ranges 1-5
Color LIGHT GREEN
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Stage Clips
-secures specimen/slide
Color GREY
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Bulb/Mirror
-light source
-NEVER use the sunlight it will hurt your eyes!!
Color YELLOW
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Scanning Objective Lens
-shortest lens-4x magnification
Color BLACK
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Low Power Objective Lens
-10x magnification
Color PURPLE
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High Power Objective Lens
-40x magnification
Color ORANGE STRIPES
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Body Tube
-allows light from objective to pass upward
Color BROWN
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Eye Piece/Ocular lens
-Site of observation-10x magnification
Color ORANGE
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Nose Piece
-Allows you to switch objective lenses
Color BLUE and GREEN stripes
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Coarse Adjustment Knob
-used to focus specimen-DO NOT use on high power objective lens
Color PINK
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Fine Adjustment Knob
-Used for minute focusing -Used after coarse adjustment knob
Color Tan
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• Total magnification = (ocular lens power) x (objective lens power)
• Example: (10x)oculear lens x (40x)high power lens = 400x total magnification
• Magnification: The ability to increase the size of an image
Ocular Lens Objective Lens
= 400x total magnification
X
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Light Pathway: Light Source→Specimen→Objective Lens→Body Tube→Eye Piece
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Resolution
• making the image clear
• ability to determine between two objects or points
• can be adjusted with the fine/course knob or the diaphragm (to allow more light in)
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Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a
higher power of magnification?
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Use of a Scope Carry the microscope with 2 hands (arm & base)
Use closest electrical outlet without going through a walkway
Looking at a slide:
Use ONLY ONE slide at a time ALWAYS start at LOW power first(10x) ALWAYS start with the stage lowered completely Make sure the objective clicks into position The course focus knob is ONLY used on the lowest
power lens The fine focus knob is used on all lenses to bring the
image into focus
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Putting the Microscope Away
Lower the stage completely and click the lowest objective into place
Return slide to tray Wind up the scope power cord
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Agenda
1. Finish Microscope Handout
• Glue in notebook when finished
• Due tomorrow
2. Study Guide
• Answer on separate piece of paper
• Due Friday
3. Extra Credit Flashcards
• Make 20
• Due Friday
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Types of Microscopes
1. Compound Light Microscope: Shines light through a specimen and uses 2 lenses for magnification
Magnification – increase in an objects apparent size
Resolution – how clear the image appears once magnified
Max clear magnification ~2000x
2. Electron microscope: Uses electrons instead of light to visualize the specimen, electrons bounce off or pass through and a computer interprets a picture
Always black and white, specimen must be dead to examine
Scanning EM – magnifies up to 100,000x
Transmission EM – magnifies up to 200,000x
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Microscope Images
Compound Light Microscope - Protozoa
Transmission Electron Microscope - Bacteria
Scanning Electron Microscope (SEM)Butterfly Tongue