Stock solutions of the MORPHEUS screens
MORPHEUS I
MORPHEUS II
MORPHEUS III
For every condition, a mix of additives, a mix of precipitants and a buffer system are combined using the following fixed ratio:
0.5 stock precipitants + 0.1 stock additives + 0.1 stock buffer system + 0.3 water
The 38 stock solutions (by alphabetical order)
The screens: Three 96-condition initial screens (3-D grids)+ Two additive screens (single reagents)
The 6 buffer systems: acid-to-base ratios for the corresponding pH range
System 1
pH MES Imidazole3.1 100 06.0 71 296.1 68 326.2 65 356.3 62 386.4 58 426.5 56 446.6 53 476.7 50 506.8 47 536.9 44 567.0 41 59
10.2 0 100
System 2
pH MOPS Na-HEPES3.4 100 07.0 72 287.1 67 337.2 63 377.3 59 417.4 55 457.5 50 507.6 46 547.7 42 587.8 37 637.9 33 678.0 29 71
10.9 0 100
System 3
pH Bicine Trizma-base5.0 100 08.0 67 338.1 62 388.2 56 448.3 50 508.4 45 558.5 39 618.6 34 668.7 28 728.8 24 768.9 19 819.0 16 84
10.8 0 100
System 4
pH MOPSO Bis-tris2.3 100 06.0 87 136.1 85 156.2 81 196.3 78 226.4 74 266.5 69 316.6 64 366.7 59 416.8 53 476.9 48 527.0 42 58
11.5 0 100
System 5
pH BES TEA2.8 100 07.0 68 327.1 65 357.2 63 387.3 60 407.4 58 427.5 56 447.6 55 457.7 53 477.8 52 487.9 51 498.0 50 50
13.1 0 100
System 6
pH Glygly AMPD5.6 100 08.0 80 208.1 78 228.2 75 258.3 72 288.4 69 318.5 66 348.6 63 378.7 61 398.8 58 428.9 55 459.0 52 48
11.9 0 100
F. Gorrec 2018
Storagetemperature
ScreeningKitName(andLMBname) RT 4°C -20°C CatalogueNumber(MDL) Comments(reagentsintegrated)
MORPHEUSI ('LMB17') ✓ MD1-46(tubes,10mL) AllreagentshighlyrepresentedinthePDB
MORPHEUSII('LMB20') ✓ MD1-91(tubes,10mL) Glycerol-likecryoprotectantsandheavy-atoms
MORPHEUSIII('LMB22') ✓ MD1-116(tubes,10mL) Drug-likecompoundsasadditives
MORPHEUSadditive('Addit01') ✓ MD1-93(plate,100μL) The95reagentsfoundinMORPHEUSI&II
HIPPOCRATES('Addit03') ✓ MD1-118(plate,100μL) The44(+3)additivesfoundinMORPHEUSIII
StockName InitialScreen CN(100/250mL) Description
Alcohols MORPHEUSI MD2-100-73 Mixof6additives(conc.0.2Meach)
Alkalis MORPHEUSII MD2-100-233 Mixof4additives(conc.10mMeach)
Aminoacids MORPHEUSI MD2-100-77 Mixof5additives(conc.0.2Meach)
AminoacidsII MORPHEUSII MD2-100-237 Mixof5additives(0.2Meach)
Anestheticalkaloids MORPHEUSIII MD2-100-320 Mixof4additives(conc.2%w/veach)
Antibiotics MORPHEUSIII MD2-100-318 Mixof6additives(conc.1%w/veach)
Buffersystem1 MORPHEUSI&III MD2-100-100 Twostocks:1MMES(acid,pKa6.2)and1Mimidazole(base,pKa6.9)
Buffersystem2 MORPHEUSI&III MD2-100-101 Twostocks:1MMOPS(acid,pKa7.3)and1MSodium-HEPES(base,pKa7.6)
Buffersystem3 MORPHEUSI&III MD2-100-102 Twostocks:1Mbicine(acid,pKa8.4)and1MTrizma-base(pKa8.20)
Buffersystem4 MORPHEUSII MD2-100-243 Twostocks:1MMOPSO(acid,pKa6.9)and1Mbis-tris(base,pKa6.5)
Buffersystem5 MORPHEUSII MD2-100-244 Twostocks:1MBES(acid,pKa7.1)and1MTEA*(base,pKa17.8)
Buffersystem6 MORPHEUSII MD2-100-245 Twostocks:1MGlyGly(acid,pKa28.2)and1MAMPD*(base,pKa8.8)
Carboxylicacids MORPHEUSI MD2-100-76 Mixof5additives(conc.0.2Meach)
Cholicacidderivatives MORPHEUSIII MD2-100-319 Mixof4additives(conc.3%w/veach)
Dipeptides MORPHEUSIII MD2-100-313 Mixof8additives(conc.2%w/veach)
DivalentcationsII MORPHEUSII MD2-100-232 Mixof4additives(5mMeach)
Divalentscations MORPHEUSI MD2-100-70 Mixof2additives(conc.0.3Meach)
EthyleneGlycols MORPHEUSI MD2-100-74 Mixof4additives(conc.0.3Meach)
Halides MORPHEUSI MD2-100-71 Mixof3additives(conc.0.3Meach)
Lanthanides MORPHEUSII MD2-100-235 Mixof4additives(5mMeach)
LiNaK MORPHEUSII MD2-100-231 Mixof3additives(SO4salts,0.3Meach)
Monosaccharides MORPHEUSI MD2-100-75 Mixof6additives(conc.0.2Meach)
MonosaccharidesII MORPHEUSII MD2-100-236 Mixof5additives(0.2Meach)
NPS MORPHEUSI MD2-100-72 Mixof3additives(NO3,PO4andSO4,conc.0.3Meach)
Nucleosides MORPHEUSIII MD2-100-315 Mixof5additives(conc.2%w/veach)
Oxometalates MORPHEUSII MD2-100-234 Mixof4additives(5mMeach)
Phytochemicals1 MORPHEUSIII MD2-100-316 Mixof7additives(conc.0.5%w/veachin50%v/v/EtOH)
Phytochemicals2 MORPHEUSIII MD2-100-317 Mixof5additives(conc.0.5%w/veachin50%v/v/EtOH)
Polyamines MORPHEUSII MD2-100-238 Mixof4additives(powder:AddDWtoreach0.1Meach)
Precipitantmix1 MORPHEUSI&III MD2-250-81 Mixof2reagents(totalconc.60%,cryo:PEGMME500)
Precipitantmix2 MORPHEUSI&III MD2-250-82 Mixof2reagents(totalconc.60%,cryo:ethyleneglycol)
Precipitantmix3 MORPHEUSI&III MD2-250-83 Mixof2reagents(totalconc.60%,cryo:glycerol)
Precipitantmix4 MORPHEUSI&III MD2-250-84 Mixof3reagents(totalconc.75%,cryo:MPD)
Precipitantmix5 MORPHEUSII MD2-250-239 Mixof3reagents(totalconc.72%,cryo:glycerol-like,NDSB256)
Precipitantmix6 MORPHEUSII MD2-250-240 Mixof2reagents(totalconc.65%,cryo:glycerol-like)
Precipitantmix7 MORPHEUSII MD2-250-241 Mixof2reagents(totalconc.60%,cryo:glycerol-like)
Precipitantmix8 MORPHEUSII MD2-250-242 Mixof3reagents(totalconc.62%,cryo:tris-like,NDSB195)
Vitamins MORPHEUSIII MD2-100-314 Mixof5additives(conc.3%w/veach)
*AMPD:2-amino-2-methyl-1,3-propanediol;TEA:triethanolamine
