Shimadzu’s Total Support for Food Safety
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© Shimadzu Corporation, 2014www.shimadzu.com/an/
C10G-E020B
Analytical and Testing Instruments for Food Safety
Shimadzu’s Total Supportfor Food Safety
List of Analytical and Testing Instruments for Food Safety
Shimadzu Balances
UniBloc family of balances
MALDI-TOF Mass Spectrometer (MALDI-TOF-MS)
Gas Chromatograph (GC)
UV-VIS Spectrophotometer(UV)
FTIR Spectrophotometer(FTIR)
Liquid Chromatograph (LC)
Gas Chromatograph Mass spectrometer (GC-MS)
Liquid Chromatograph Mass spectrometer (LC-MS)
Atomic Absorption Spectrophotometer (AA)
ICP Emission / Spectrometer (ICP, ICP-MS)
X-Ray Fluorescence Spectrometer (XRF, EDX)
Universal Testing Machine (AG, EZ Test)
ResidualPesticides
MycotoxinsVeterinary
PharmaceuticalFood
AdditiveToxic
Metals
Inspection andAnalysis of
Foreign MatterOff-Flavor
Prevention ofDefects inPackaging
Region andType
Identification
Identificationand Observation
of Bacteria
World Map of Shimadzu Sales, Service,Manufacturing, and R&D Facilities
Sales and Service
Manufacturing
R&D
In addition to offering analytical instruments and testing
machines, Shimadzu provides total support that includes the
provision of information for the food safety community,
training at workshops and seminars, and instrument
maintenance management.
Shimadzu's Total Support for Food Safety
Contents
P.4
P.7
P.8
P.9
P.10
P.11
P.12
P.13
P.14
P.15
P.16
Analysis of Residual Pesticides
Analysis of Mycotoxins
Identification and Observation of Microorganisms
Analysis of Veterinary Pharmaceuticals
Analysis of Food Additives
Analysis of Toxic Metals
Inspection and Analysis of Foreign Matter
Food Processing
Analysis of Flavor & Texture
Prevention of Defects in Packaging
List of Food Testing Application by Instrument
Shimadzu provides total support for food safety, whether it is farm to
fork or bait to plate. As a leading manufacturer of a wide range of
analytical instruments, Shimadzu undertakes development of new
instruments and technologies, and provides comprehensive service
support in order to keep up with changing market demands.
World Map of Shimadzu Sales, Service,Manufacturing, and R&D Facilities
Sales and Service
Manufacturing
R&D
In addition to offering analytical instruments and testing
machines, Shimadzu provides total support that includes the
provision of information for the food safety community,
training at workshops and seminars, and instrument
maintenance management.
Shimadzu's Total Support for Food Safety
Contents
P.4
P.7
P.8
P.9
P.10
P.11
P.12
P.13
P.14
P.15
P.16
Analysis of Residual Pesticides
Analysis of Mycotoxins
Identification and Observation of Microorganisms
Analysis of Veterinary Pharmaceuticals
Analysis of Food Additives
Analysis of Toxic Metals
Inspection and Analysis of Foreign Matter
Food Processing
Analysis of Flavor & Texture
Prevention of Defects in Packaging
List of Food Testing Application by Instrument
Shimadzu provides total support for food safety, whether it is farm to
fork or bait to plate. As a leading manufacturer of a wide range of
analytical instruments, Shimadzu undertakes development of new
instruments and technologies, and provides comprehensive service
support in order to keep up with changing market demands.
4 5Shimadzu’s Total Support for Food Safety
Analytical and Testing Instruments for Food Safety
Analysis of Residual Pesticides
MRM Analysis of Residual Pesticides in Food (GC-MS/MS)
Liquid Chromatograph Mass Spectrometer (LC-MS/MS)
Liquid Chromatograph Mass Spectrometer (LC-MS)
Triple Quadrupole Mass SpectrometerGCMS-TQ8040
Triple Quadrupole Mass SpectrometerLCMS-8050
Single Quadrupole Mass SpectrometerLCMS-2020
Single Quadrupole Mass SpectrometerGCMS-QP2010 Ultra
Analysis of 360 pesticides by GC-MS/MS in less than 10 minutes with Smart MRMSimultaneous analysis of 226 pesticides (10ng/mL) within two minutes using LC-MS/MS
Chromatograms of 8 Water-Soluble Vitamins
Features
So Smart it almost runs itself!
Smart productivity
• 400+ compounds in one run
• Smart MRM software
• Twin Line MS Kit
Smart operation
• MRM optimization tool
• Smart Database series
• Automatic Method Creation
Smart performance
• Scan/MRM acquisition mode
• High-speed scanning
• Automatic Adjustment of Retention Time (AART).
Features
Options
Analysis of 360 pesticides in apple extract in 10 minutes using a
GCMS-TQ8040 with Smart MRM.
Food knows no borders. A pesticide used in one country may be banned in another country, or even banned around the world
because of health risks. This has resulted in increased testing of pesticides in agricultural products as there are over 1,000
known pesticides. Shimadzu offers a wide range of instruments for pesticide testing, from GC and LC with specific detectors
to single and triple quadrupole mass spectrometers.
Finally, a triple quadrupole GC-MS/MS Smart enough for everyday use in your laboratory. The Shimadzu GCMS-TQ8040 is the
first triple quadrupole with Smart Productivity for high-efficiency sample throughput, Smart Operation for quick and easy
method development, and Smart Performance for low detection limits and Scan/MRM. These three smart technologies
contribute to Smart MRM and provide the most accurate, cost-effective, and easy-to-use triple quadrupole GCMS you have
ever imagined.
Smart Pesticide Database
The Smart Pesticide Database includes optimized transitions and
collision energies, CAS registry numbers, and Retention Indices (RI)
for hundreds of pesticides. Select from pre-registered compounds in
one database, or add you own optimized transitions. Select the
compounds to be analyzed, and Smart MRM builds the MRM or
Scan/MRM acquisition method from the “Smart Database” with a
push of a button.
Pesticide Quick-DB
The software package contains Scan/MRM and Scan/SIM acquisition
methods, and pre-stored calibration curves for semi-quantitative
results for 480 pesticides within one hour of installation.
LC-MS/MS MRM (Multiple Reaction Monitoring) analysis allows
for rapid and sensitive detection of pesticides in complex
matrices. An example of simultaneous multi-residue multi-class
pesticide analysis is shown below.
Single quadrupole LC-MS enables rapid and sensitive
quantitative analysis of a wide variety of analytes. An example
of vitamin analysis is shown below.
High sensitivity
The high-performance ion source improves and lowers the minimum
limits of detection and quantitation for measurements of trace
components. A reduced absolute sample injection volume prevents
contamination of the insert.
Simultaneous Scan/SIM measurements
During high-sensitivity SIM measurements of trace components,
Scan measurements are performed on unknown components missed
by SIM.
Retention time correction
Significantly reduces the workload for multicomponent
simultaneous analysis.
Features
Ultra-high speed
Ultra-fast polarity switching at 5 msec.
Ultra-fast scanning at 30,000 u/sec.
Ultra-fast MRM transition speeds at 555 MRMs/sec.
High sensitivity
Newly improved ion optics and collision cell provide higher MRM
and Scan sensitivity, which expands the potential range of LC-MS/MS
applications.
User -friendly
Minimize instrument downtime with easy maintenance.
A single-vendor solution of LC and MS provides seamless operation.
In addition, it provides for simplistic MRM optimization.
Rapid polarity switching (15 msec) allows detection of both positively and
negatively charged species. Excellent linearity (R2 > 0.99) and 1-50
part-per-billion quantitation limits were obtained.
0.0 2.5 5.0 7.5 10.0 12.5 min
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5(×1,000,000)
1 Thiamin
2 Pyridoxine
3 Nicotinic acid
4 Nicotinamide
5 Pantothenic acid
6 Cyanocobalamin
8 Folic acid
7 Riboflavin
(+):TIC(+):265.10(+):170.20(+):124.20(2.90)(+):123.20(1.12)(+):220.20(1.92)(+):678.60(5.56)(+):377.20(2.95)(-):440.10(50.00)
0.25
Inte
nsi
ty
0
0.5
1.0
1.5
x105
0.5 0.75 1.00 1.25 1.5 1.75 min
4 5Shimadzu’s Total Support for Food Safety
Analytical and Testing Instruments for Food Safety
Analysis of Residual Pesticides
MRM Analysis of Residual Pesticides in Food (GC-MS/MS)
Liquid Chromatograph Mass Spectrometer (LC-MS/MS)
Liquid Chromatograph Mass Spectrometer (LC-MS)
Triple Quadrupole Mass SpectrometerGCMS-TQ8040
Triple Quadrupole Mass SpectrometerLCMS-8050
Single Quadrupole Mass SpectrometerLCMS-2020
Single Quadrupole Mass SpectrometerGCMS-QP2010 Ultra
Analysis of 360 pesticides by GC-MS/MS in less than 10 minutes with Smart MRMSimultaneous analysis of 226 pesticides (10ng/mL) within two minutes using LC-MS/MS
Chromatograms of 8 Water-Soluble Vitamins
Features
So Smart it almost runs itself!
Smart productivity
• 400+ compounds in one run
• Smart MRM software
• Twin Line MS Kit
Smart operation
• MRM optimization tool
• Smart Database series
• Automatic Method Creation
Smart performance
• Scan/MRM acquisition mode
• High-speed scanning
• Automatic Adjustment of Retention Time (AART).
Features
Options
Analysis of 360 pesticides in apple extract in 10 minutes using a
GCMS-TQ8040 with Smart MRM.
Food knows no borders. A pesticide used in one country may be banned in another country, or even banned around the world
because of health risks. This has resulted in increased testing of pesticides in agricultural products as there are over 1,000
known pesticides. Shimadzu offers a wide range of instruments for pesticide testing, from GC and LC with specific detectors
to single and triple quadrupole mass spectrometers.
Finally, a triple quadrupole GC-MS/MS Smart enough for everyday use in your laboratory. The Shimadzu GCMS-TQ8040 is the
first triple quadrupole with Smart Productivity for high-efficiency sample throughput, Smart Operation for quick and easy
method development, and Smart Performance for low detection limits and Scan/MRM. These three smart technologies
contribute to Smart MRM and provide the most accurate, cost-effective, and easy-to-use triple quadrupole GCMS you have
ever imagined.
Smart Pesticide Database
The Smart Pesticide Database includes optimized transitions and
collision energies, CAS registry numbers, and Retention Indices (RI)
for hundreds of pesticides. Select from pre-registered compounds in
one database, or add you own optimized transitions. Select the
compounds to be analyzed, and Smart MRM builds the MRM or
Scan/MRM acquisition method from the “Smart Database” with a
push of a button.
Pesticide Quick-DB
The software package contains Scan/MRM and Scan/SIM acquisition
methods, and pre-stored calibration curves for semi-quantitative
results for 480 pesticides within one hour of installation.
LC-MS/MS MRM (Multiple Reaction Monitoring) analysis allows
for rapid and sensitive detection of pesticides in complex
matrices. An example of simultaneous multi-residue multi-class
pesticide analysis is shown below.
Single quadrupole LC-MS enables rapid and sensitive
quantitative analysis of a wide variety of analytes. An example
of vitamin analysis is shown below.
High sensitivity
The high-performance ion source improves and lowers the minimum
limits of detection and quantitation for measurements of trace
components. A reduced absolute sample injection volume prevents
contamination of the insert.
Simultaneous Scan/SIM measurements
During high-sensitivity SIM measurements of trace components,
Scan measurements are performed on unknown components missed
by SIM.
Retention time correction
Significantly reduces the workload for multicomponent
simultaneous analysis.
Features
Ultra-high speed
Ultra-fast polarity switching at 5 msec.
Ultra-fast scanning at 30,000 u/sec.
Ultra-fast MRM transition speeds at 555 MRMs/sec.
High sensitivity
Newly improved ion optics and collision cell provide higher MRM
and Scan sensitivity, which expands the potential range of LC-MS/MS
applications.
User -friendly
Minimize instrument downtime with easy maintenance.
A single-vendor solution of LC and MS provides seamless operation.
In addition, it provides for simplistic MRM optimization.
Rapid polarity switching (15 msec) allows detection of both positively and
negatively charged species. Excellent linearity (R2 > 0.99) and 1-50
part-per-billion quantitation limits were obtained.
0.0 2.5 5.0 7.5 10.0 12.5 min
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5(×1,000,000)
1 Thiamin
2 Pyridoxine
3 Nicotinic acid
4 Nicotinamide
5 Pantothenic acid
6 Cyanocobalamin
8 Folic acid
7 Riboflavin
(+):TIC(+):265.10(+):170.20(+):124.20(2.90)(+):123.20(1.12)(+):220.20(1.92)(+):678.60(5.56)(+):377.20(2.95)(-):440.10(50.00)
0.25
Inte
nsi
ty
0
0.5
1.0
1.5
x105
0.5 0.75 1.00 1.25 1.5 1.75 min
6 7Shimadzu’s Total Support for Food Safety
Analytical and Testing Instruments for Food Safety
Analysis of Residual Pesticides Analysis of Mycotoxins
Analysis of Residual Pesticides (GC)
Gas Chromatograph GC-2010 Plus
Chromatogram of a Standard Mixture of 10 N-Methylcarbamate Pesticides
Chromatograms of normal meat sample and pesticides-spiked meat sample
Chromatogram of 45 mycotoxins at 50 ppb (2 ppb for aflatoxins and ochratoxines)
Analysis of aflatoxins in wheat flour
B1, G1 : 20ng/LB2, G2 : 5ng/L
SIL-30ACMP Multi-Plate Autosampler
Carryover evaluation of fumonisins
Capillary GC can be utilized for the analysis of residual
pesticides in foods. Using a selective detector such as FPD,
FTD or ECD offers highly sensitive analysis of trace pesticides.
Liquid Chromatograph (LC)
GC or GC-MS techniques may not be suitable for analysis of
residual pesticides that thermally decompose or have a
highly polar chemical structure. In contrast, an HPLC
equipped with a photodiode array detector can acquire
reliable chromatograms with spectral information for
building libraries to compare compounds. The Shimadzu
carbamate analysis system incorporates derivatization
technologies to achieve high-sensitivity, selective analysis of
N-methyl carbamate pesticides.
Simultaneous Analysis of Mycotoxins (LC-MS/MS)
Analysis of Aflatoxins (UHPLC) Ultra High Performance Liquid ChromatographNexera X2 with RF-20Axs
LC-MS/MS is effective for analysis of mycotoxins in complex
matrices. Shown is an example of simultaneous analysis of 45
mycotoxins by LC-MS/MS. All compounds, including positive
and negative ions, were separated and detected with high
sensitivity within 9 minutes. For analysis of mycotoxins with
high sensitivity, eliminating carryover is indispensable.
Modified rinse methods using the SIL-30AC autosampler can
eliminate carryover of fumonisin.
The analysis of aflatoxins (B1, B2, G1 and G2) is very
important from a food safety perspective due to concerns
about adverse effects on human health. The Nexera Ultra
High Performance Liquid Chromatograph achieves
high-resolution analysis of aflatoxins in less than four
minutes. The RF-20Axs high-sensitivity fluorescence detector
allows direct analysis of aflatoxins at trace levels (ppt)
without any derivatization.
Features
High-sensitivity detection
The FPD (Flame Photometric Detector) provides powerful detection
of phosphorous and sulfur-based pesticides. For example, the
minimum detectable quantities of phosphorus and sulfur are 55
fgP/s and 3 fgS/s, respectively.
Higher analytical efficiency and productivity
With the addition of a new cooling fan and superior air flow
optimization, the oven cooling speed has dramatically improved
(450 °C -> 50 °C cooling time: 3.4 minutes). A self-diagnostic
function prevents failure by confirming proper operation and
periodic diagnostics prevent unexpected downtime.
Multiple detectors in a single GC
Three detectors can be simultaneously mounted in the GC-2010 Plus:
ECD, effective for chlorinated and pyrethroid-based pesticides; FPD,
effective for sulfur- and phosphorous-based pesticides; and FTD,
effective for nitrogen- and phosphorous-based pesticides.
Features
Rapid injection
An injection time of 10 seconds for a 10 µL sample enhances analysis
efficiency.
High sensitivity and linearity over a broad range
Upgraded optical and electrical systems reduce noise for lower
detection limits and ensure simultaneous analysis of major
components and trace components.
Low sample carryover
Platinum coating minimizes the adsorption of sample components
on to the needle surface.
In a batch analysis of multiple samples, compounds with greatly
differing polarities are frequently analyzed together. In cases such as
this, sufficient rinsing between samples to reduce carryover may not
be achieved using one type of rinse solution. To combat this, the
SIL-30ACMP design incorporates sample-contacting materials to which
components are less likely to adsorb, and a multi-solvent internal and
external needle and loop rinsing protocol designed to reduce
carryover to less than 0.0015%.
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Pesticide-spiked meat dumpling sample (0.2 µ g/g equivalent)
Normal meat dumpling sample
LCMS-8050
High-Performance Liquid Chromatograph Prominence SeriesCarbamates Pesticides Analysis System
1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 min-500000
0
500000
1000000
1500000
2000000
2500000
3000000
3500000
4000000
4500000
5000000
5500000
6000000
6500000
7000000
7500000
8000000
8500000
9000000
9500000
10000000
10500000
11000000
11500000
12000000
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0.0 5.0 10.0 15.0 20.0 25.0 min
0
10
20
30
40
50
60
mV
6 7Shimadzu’s Total Support for Food Safety
Analytical and Testing Instruments for Food Safety
Analysis of Residual Pesticides Analysis of Mycotoxins
Analysis of Residual Pesticides (GC)
Gas Chromatograph GC-2010 Plus
Chromatogram of a Standard Mixture of 10 N-Methylcarbamate Pesticides
Chromatograms of normal meat sample and pesticides-spiked meat sample
Chromatogram of 45 mycotoxins at 50 ppb (2 ppb for aflatoxins and ochratoxines)
Analysis of aflatoxins in wheat flour
B1, G1 : 20ng/LB2, G2 : 5ng/L
SIL-30ACMP Multi-Plate Autosampler
Carryover evaluation of fumonisins
Capillary GC can be utilized for the analysis of residual
pesticides in foods. Using a selective detector such as FPD,
FTD or ECD offers highly sensitive analysis of trace pesticides.
Liquid Chromatograph (LC)
GC or GC-MS techniques may not be suitable for analysis of
residual pesticides that thermally decompose or have a
highly polar chemical structure. In contrast, an HPLC
equipped with a photodiode array detector can acquire
reliable chromatograms with spectral information for
building libraries to compare compounds. The Shimadzu
carbamate analysis system incorporates derivatization
technologies to achieve high-sensitivity, selective analysis of
N-methyl carbamate pesticides.
Simultaneous Analysis of Mycotoxins (LC-MS/MS)
Analysis of Aflatoxins (UHPLC) Ultra High Performance Liquid ChromatographNexera X2 with RF-20Axs
LC-MS/MS is effective for analysis of mycotoxins in complex
matrices. Shown is an example of simultaneous analysis of 45
mycotoxins by LC-MS/MS. All compounds, including positive
and negative ions, were separated and detected with high
sensitivity within 9 minutes. For analysis of mycotoxins with
high sensitivity, eliminating carryover is indispensable.
Modified rinse methods using the SIL-30AC autosampler can
eliminate carryover of fumonisin.
The analysis of aflatoxins (B1, B2, G1 and G2) is very
important from a food safety perspective due to concerns
about adverse effects on human health. The Nexera Ultra
High Performance Liquid Chromatograph achieves
high-resolution analysis of aflatoxins in less than four
minutes. The RF-20Axs high-sensitivity fluorescence detector
allows direct analysis of aflatoxins at trace levels (ppt)
without any derivatization.
Features
High-sensitivity detection
The FPD (Flame Photometric Detector) provides powerful detection
of phosphorous and sulfur-based pesticides. For example, the
minimum detectable quantities of phosphorus and sulfur are 55
fgP/s and 3 fgS/s, respectively.
Higher analytical efficiency and productivity
With the addition of a new cooling fan and superior air flow
optimization, the oven cooling speed has dramatically improved
(450 °C -> 50 °C cooling time: 3.4 minutes). A self-diagnostic
function prevents failure by confirming proper operation and
periodic diagnostics prevent unexpected downtime.
Multiple detectors in a single GC
Three detectors can be simultaneously mounted in the GC-2010 Plus:
ECD, effective for chlorinated and pyrethroid-based pesticides; FPD,
effective for sulfur- and phosphorous-based pesticides; and FTD,
effective for nitrogen- and phosphorous-based pesticides.
Features
Rapid injection
An injection time of 10 seconds for a 10 µL sample enhances analysis
efficiency.
High sensitivity and linearity over a broad range
Upgraded optical and electrical systems reduce noise for lower
detection limits and ensure simultaneous analysis of major
components and trace components.
Low sample carryover
Platinum coating minimizes the adsorption of sample components
on to the needle surface.
In a batch analysis of multiple samples, compounds with greatly
differing polarities are frequently analyzed together. In cases such as
this, sufficient rinsing between samples to reduce carryover may not
be achieved using one type of rinse solution. To combat this, the
SIL-30ACMP design incorporates sample-contacting materials to which
components are less likely to adsorb, and a multi-solvent internal and
external needle and loop rinsing protocol designed to reduce
carryover to less than 0.0015%.
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EPN p
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aph
enth
ion
ph
osm
et
ph
osa
lon
e
pyr
aclo
fos
Pesticide-spiked meat dumpling sample (0.2 µ g/g equivalent)
Normal meat dumpling sample
LCMS-8050
High-Performance Liquid Chromatograph Prominence SeriesCarbamates Pesticides Analysis System
1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 min-500000
0
500000
1000000
1500000
2000000
2500000
3000000
3500000
4000000
4500000
5000000
5500000
6000000
6500000
7000000
7500000
8000000
8500000
9000000
9500000
10000000
10500000
11000000
11500000
12000000
Oxa
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0.0 5.0 10.0 15.0 20.0 25.0 min
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20
30
40
50
60
mV
8 9
Identification and Observation of Microorganisms
Shimadzu’s Total Support for Food SafetyAnalytical and Testing Instruments for Food Safety
Identification of Microorganisms (MALDI-TOF-MS)
Comparing mass spectra of different bacteria
In order to prevent pathogenic germs from contaminating
food sources, it is essential to identify them quickly and
reliably in order to allow for proper control measures.
A microorganism identification system using the AXIMA
MALDI-TOF MS enables direct measurement of cultured
microorganisms and identification within two minutes.
Analysis of Beta-agonist (LC-MS/MS)
Beta-agonists can be added to animal feed to work as
growth promoters due to their ability to increase muscle
mass and decrease fat deposition. However, due to their
adverse effects on human health, these compounds have
been banned for growth-promoting purposes in farm
animals by regulatory agencies worldwide.
Analysis of Anabolic Steroids (On-line SPE-LC-MS/MS)
The use of natural and synthetic hormones to increase the
weight of meat-producing animals is prohibited by
regulatory agencies worldwide in order to protect consumers
from the harmful effects of digesting hormone residues and
their metabolites. Compliance with this legislation is
monitored by the screening of a large number of animals
every year.
Analysis of Malachite Green and Leucomalachite Green in Fish (LC-MS)
Malachite green is an organic dye used as an antimicrobial
medication to treat white-spot disease and tail rot in
aquarium fish. Its use is not authorized for farmed fish in
some countries. As malachite green is metabolized to
leucomalachite green in the human body, the simultaneous
analysis of these two components is required. Below is an
example of the LC-MS analysis of malachite green and
leucomalachite green.
Analysis of Chloramphenicol in Honey (LC-MS)
Chloramphenicol is effective against many microorganisms.
However, due to its serious side effects in humans, it is only
used against potentially fatal infectious diseases.
Chloramphenicol has not only been detected in fish and
meat, but cases are occasionally reported of the detection of
chloramphenicol in honey and royal jelly.What is Microorganism Identification by MALDI-TOF MS?
Species-specific mass spectral patterns from microorganisms can be
obtained by direct measurement of microorganisms using MALDI-TOF
MS. Identification of microorganisms can be performed by matching
these collected spectra to the database. This is a fast, easy and
reliable method for identification of microorganisms.
In the event of food poisoning, identification of the causative factor is extremely important in determining the path of
infection, prescribing treatment and preventing recurrence. Shimadzu’s iDPlus microorganism identification platform provides
rapid identification of microorganisms in food.
Analysis of Veterinary Pharmaceuticals
Veterinary pharmaceuticals are used in the diagnosis, cure, mitigation, treatment, and prevention of diseases in animals as
well as in the promotion of growth. However, there is increasing concern about these drugs entering the food chain and their
effects on the human body. These drugs are categorized in many classes, such as anabolic hormones, anthelmintics,
antibiotics, beta-agonists, coccidiostats, corticosteroids, nonsteroidal anti-inflammatory drugs (NSAIDs), and sedatives. Below
are a few examples of veterinary pharmaceutical analyses. AXIMA iDPlus Microorganism Identification System
Quick and easy analysis
Three steps from sample preparation to identification.
The microorganism identification process is finished within two
minutes after starting the analysis.
Highly accurate identification
Microorganism identification by MALDI-TOF MS is a well-established
technique with a low number of false positives.
Applicable for a wide range of microorganisms
Capable of classifying/identifying gram-positive and gram-negative
bacteria, yeasts, fungi and spores. iDPlus identifies mircoorganisms
by family, genus, species, and subspecies.
Database customization
Easily register new microorganisms in the database, which allows
rapid development of a high-quality database.
Low operational cost
Analysis can be performed for pennies per sample compared to
expensive traditional techniques.
Expanded research capabilities
iDPlus is not limited to microorganism identification. Reflectron
mode and MS/MS functionalities enable a true research platform.
SRM chromatograms of 15 beta-agonists extracted from bovine liver (0.5 x screening target concentration). All compounds were eluted within 3.7 minutes.
Analysis of eight target steroids in less than 4.5 minutes: Peak #1 β-boldenone,#2 α-trenbolone, #3 Dienestrol, #4 Testosterone, #5 α-boldenone,#6 Hydroxystanozolol, #7 methyltestosterone, #8 Progesterone
SIM Chromatograms of Malachite Green and Leucomalachite Green
Chromatogram of honey samples unspiked and spiked with chloramphenicol
MS spectrum from LC-MS analysis of chloramphenicol
275.0 300.0 325.0 350.0 375.0 m/z0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
Inten.(X10,000)321
359
367384
m/z 321 [M-H]-
m/z 357 [M+Cl]-
6.0 7.0 8.0 9.0
7.25
7.50
7.75
8.00
8.25
8.50
(×1,000)
min
% Int.
4000 6000 8000 10000 12000 14000
m/z
6885
.93
5524
.87
5033
.17
4308
.68
7230
.02
6352
.79
6043
.95
6671
.91
5209
.27
4434
.57
9059
.12
8348
.89
5255
.03
5369
.13
9733
.78
9235
.80
4307
.86
6091
.59
4365
.39
7156
.44
7886
.39
6253
.14
7867
.55
7590
.49
7658
.66
3890
.60
7158
.16
1142
4.07
3829
.04
3640
.52
1095
0.18
4054
.41
8364
.74
8871
.39
1229
5.04
1022
0.70
9520
.27
1102
7.94
1369
8.41
13
762.
64
1499
1.82
1192
2.76
1239
7.15
12
706.
04
1322
5.69
13
645.
06
1406
7.89
Bacillus subtilis
Escherichia coli
Pseudomonas aeruginosa
Staphylococcus aureus subsp. Aureus
Salmonella enterica subsp. Enterica
Features
Cim
ater
ol
Terb
uta
line
Salb
uta
mo
lZi
lpat
ero
l Cim
bu
tero
l
Hyd
roxy
met
hyl
clen
bu
tero
l C
len
pro
per
ol
Rac
top
amin
e
Cle
nb
ute
rol
Tulo
bu
tero
lB
rom
ob
ute
rol M
abu
tero
lIs
oxs
up
rin
eC
len
pen
tero
l
Map
ente
rol
A: Water 0.2mM ammonium fluorideB: Methanol 0.2mM ammonium fluoride
12
3
4
5
6
7
8
Malachite Green
Leucomalachite Green
8 9
Identification and Observation of Microorganisms
Shimadzu’s Total Support for Food SafetyAnalytical and Testing Instruments for Food Safety
Identification of Microorganisms (MALDI-TOF-MS)
Comparing mass spectra of different bacteria
In order to prevent pathogenic germs from contaminating
food sources, it is essential to identify them quickly and
reliably in order to allow for proper control measures.
A microorganism identification system using the AXIMA
MALDI-TOF MS enables direct measurement of cultured
microorganisms and identification within two minutes.
Analysis of Beta-agonist (LC-MS/MS)
Beta-agonists can be added to animal feed to work as
growth promoters due to their ability to increase muscle
mass and decrease fat deposition. However, due to their
adverse effects on human health, these compounds have
been banned for growth-promoting purposes in farm
animals by regulatory agencies worldwide.
Analysis of Anabolic Steroids (On-line SPE-LC-MS/MS)
The use of natural and synthetic hormones to increase the
weight of meat-producing animals is prohibited by
regulatory agencies worldwide in order to protect consumers
from the harmful effects of digesting hormone residues and
their metabolites. Compliance with this legislation is
monitored by the screening of a large number of animals
every year.
Analysis of Malachite Green and Leucomalachite Green in Fish (LC-MS)
Malachite green is an organic dye used as an antimicrobial
medication to treat white-spot disease and tail rot in
aquarium fish. Its use is not authorized for farmed fish in
some countries. As malachite green is metabolized to
leucomalachite green in the human body, the simultaneous
analysis of these two components is required. Below is an
example of the LC-MS analysis of malachite green and
leucomalachite green.
Analysis of Chloramphenicol in Honey (LC-MS)
Chloramphenicol is effective against many microorganisms.
However, due to its serious side effects in humans, it is only
used against potentially fatal infectious diseases.
Chloramphenicol has not only been detected in fish and
meat, but cases are occasionally reported of the detection of
chloramphenicol in honey and royal jelly.What is Microorganism Identification by MALDI-TOF MS?
Species-specific mass spectral patterns from microorganisms can be
obtained by direct measurement of microorganisms using MALDI-TOF
MS. Identification of microorganisms can be performed by matching
these collected spectra to the database. This is a fast, easy and
reliable method for identification of microorganisms.
In the event of food poisoning, identification of the causative factor is extremely important in determining the path of
infection, prescribing treatment and preventing recurrence. Shimadzu’s iDPlus microorganism identification platform provides
rapid identification of microorganisms in food.
Analysis of Veterinary Pharmaceuticals
Veterinary pharmaceuticals are used in the diagnosis, cure, mitigation, treatment, and prevention of diseases in animals as
well as in the promotion of growth. However, there is increasing concern about these drugs entering the food chain and their
effects on the human body. These drugs are categorized in many classes, such as anabolic hormones, anthelmintics,
antibiotics, beta-agonists, coccidiostats, corticosteroids, nonsteroidal anti-inflammatory drugs (NSAIDs), and sedatives. Below
are a few examples of veterinary pharmaceutical analyses. AXIMA iDPlus Microorganism Identification System
Quick and easy analysis
Three steps from sample preparation to identification.
The microorganism identification process is finished within two
minutes after starting the analysis.
Highly accurate identification
Microorganism identification by MALDI-TOF MS is a well-established
technique with a low number of false positives.
Applicable for a wide range of microorganisms
Capable of classifying/identifying gram-positive and gram-negative
bacteria, yeasts, fungi and spores. iDPlus identifies mircoorganisms
by family, genus, species, and subspecies.
Database customization
Easily register new microorganisms in the database, which allows
rapid development of a high-quality database.
Low operational cost
Analysis can be performed for pennies per sample compared to
expensive traditional techniques.
Expanded research capabilities
iDPlus is not limited to microorganism identification. Reflectron
mode and MS/MS functionalities enable a true research platform.
SRM chromatograms of 15 beta-agonists extracted from bovine liver (0.5 x screening target concentration). All compounds were eluted within 3.7 minutes.
Analysis of eight target steroids in less than 4.5 minutes: Peak #1 β-boldenone,#2 α-trenbolone, #3 Dienestrol, #4 Testosterone, #5 α-boldenone,#6 Hydroxystanozolol, #7 methyltestosterone, #8 Progesterone
SIM Chromatograms of Malachite Green and Leucomalachite Green
Chromatogram of honey samples unspiked and spiked with chloramphenicol
MS spectrum from LC-MS analysis of chloramphenicol
275.0 300.0 325.0 350.0 375.0 m/z0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
Inten.(X10,000)321
359
367384
m/z 321 [M-H]-
m/z 357 [M+Cl]-
6.0 7.0 8.0 9.0
7.25
7.50
7.75
8.00
8.25
8.50
(×1,000)
min
% Int.
4000 6000 8000 10000 12000 14000
m/z
6885
.93
5524
.87
5033
.17
4308
.68
7230
.02
6352
.79
6043
.95
6671
.91
5209
.27
4434
.57
9059
.12
8348
.89
5255
.03
5369
.13
9733
.78
9235
.80
4307
.86
6091
.59
4365
.39
7156
.44
7886
.39
6253
.14
7867
.55
7590
.49
7658
.66
3890
.60
7158
.16
1142
4.07
3829
.04
3640
.52
1095
0.18
4054
.41
8364
.74
8871
.39
1229
5.04
1022
0.70
9520
.27
1102
7.94
1369
8.41
13
762.
64
1499
1.82
1192
2.76
1239
7.15
12
706.
04
1322
5.69
13
645.
06
1406
7.89
Bacillus subtilis
Escherichia coli
Pseudomonas aeruginosa
Staphylococcus aureus subsp. Aureus
Salmonella enterica subsp. Enterica
Features
Cim
ater
ol
Terb
uta
line
Salb
uta
mo
lZi
lpat
ero
l Cim
bu
tero
l
Hyd
roxy
met
hyl
clen
bu
tero
l C
len
pro
per
ol
Rac
top
amin
e
Cle
nb
ute
rol
Tulo
bu
tero
lB
rom
ob
ute
rol M
abu
tero
lIs
oxs
up
rin
eC
len
pen
tero
l
Map
ente
rol
A: Water 0.2mM ammonium fluorideB: Methanol 0.2mM ammonium fluoride
12
3
4
5
6
7
8
Malachite Green
Leucomalachite Green
10 11
Analysis of Food Additives
Shimadzu’s Total Support for Food SafetyAnalytical and Testing Instruments for Food Safety
A wide variety of additives are used in foods such as preservatives, flavor enhancers, coloring agents and antioxidants.
Strict monitoring of food stuffs is essential to detect the use of prohibited chemicals. Each country assigns a unique number or
code of approved chemicals for that country. Shimadzu offers a wide range of products for detecting the prohibited
chemicals, whether they were added intentionally or through contamination.
Analysis of Toxic Metals
Toxic metals (arsenic, lead, cadmium, etc.) in foods must be controlled down to low concentration levels.
Shimadzu atomic absorption spectrophotometers and ICP emission spectrometers offer highly sensitive analysis of trace
elements in foods. Shimadzu’s product lineup also includes X-ray fluorescence spectrometers that can perform elemental
analysis without sample pretreatment. These instruments are ideal for screening analysis and emergency analysis.
Melamine was found to be used as a protein-rich adulterant
in pet food in 2007, and then in infant formula in 2008 in
China. The outbreak of the melamine scandal that killed
many dogs and cats as well as led to the deaths of six infants
and illness of many others caused panic and great concerns
worldwide. Recently, low levels of dicyandiamide (DCD)
residues were found in milk products from New Zealand.
Instead of being added directly as an adulterant, the trace
DCD found in milk products was explained to be relating to
the grass “contaminated by DCD”. Dicyandiamide has been
used to promote the growth of pastures for cows grazing.
Analysis of Dicyandiamide and Melamine in Milk Powders (LC-MS/MS) Analysis of Cd and Pb in Food Additives (AA) Atomic Absorption Spectrophotometer AA-7000
ICP Emission Spectrometer ICPE-9800 Series
Analysis of Preservatives in Food (UV)
UV-VIS Spectrophotometer UV-1800
Calibration curves of DCD and melamine Peak profiles of Cd and Pb
Quantitative results of fish CRM DORM-4
Fish Protein Certified Reference Material for Trace Metals (DORM-4) was used as fish sample.Peak profiles of Cd and Cr
Measurement results of Cd and Pb in α-cyclodextrin
Measured value
Spike and recovery rate
Element Cd
<0.003 µg/g
105 %
Pb
<0.07 µg/g
99 %
Sodium Nitrite in Meat Sorbic Acid in a Processed White Fish Product
Sudan Dyes in Curry Powder
Overlapping of six MRM peaks of 0.5 ng/mL DCD and melamine in milk powder matrix
Below are examples of the analysis of food samples spiked with preservatives.
Analysis of Sudan Dyes in Curry Powder (LC)
Sudan dyes are oil-soluble synthetic dyes used in industrial products. Their use is not authorized in food products in some countries. This is an example of LC analysis of Sudan dyes in curry powder.
Analysis of cadmium (Cd) and lead (Pb) in α-cyclodextrin
(cyclic oligosaccharide), a substance used in functional foods,
pharmaceuticals, cosmetics, etc. The analysis was conducted
by the AA-7000 atomic absorption spectrophotometer.
Analysis of Trace Element Contamination in Fish Sample (ICP)
Fish Protein Certified Reference Material for Trace Metals
(DORM-4) was used as fish sample. The Shimadzu ICPE-9800
Series can provide a rapid method to simultaneously analyze
trace elements in fish. The results show excellent correlation
with the certified reference material.
Features
High resolution
1 nm resolution provides for high data
reliability.
Enhanced security
Functions can be restricted according to
user level.
Instrument validation
Automatic/semi-automatic validation of
wavelength accuracy and wavelength
reproducibility.
Features
Measures from trace levels to high concentrations
Automatic switching between flame and furnace measurements. A
single instrument handles analysis from ppb to ppm levels.
Sophisticated automation
Automation functions enhance analysis efficiency. For instance,
automatic searching sets the optimal gas flow rate and burner
height for flame measurements. Also enables automatic dilution
and re-measurement for over ranged samples compared to the
calibration curve. The autosampler allows automatic addition of
spikes, matrix matching, and dilutions (300x) with true mixing
capability.
Advanced safety technology
World's first AA with vibration sensor to automatically extinguish
the flame in case of an earthquake. Automatic gas-leak checks and
safety functions prevent misuse of the wrong burner head.
Features
Simultaneous multi-element quantitative analysis
Multiple elements can be analyzed simultaneously.
High sensitivity and wide dynamic range
Simultaneous analysis of trace components and major components
Low running costs
Shimadzu's unique mini torch halves argon gas consumption.
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.5µg
0.4µg
0.3µg
0.2µg
0.1µg
nm
Ab
s.
Sample
450 500 550 600 650
0.7
0.6
0.5
0.4
0.3
0.2
0.1
nm
8µg
6µg
4µg
2µg
Ab
s.
250 300 350
Peaks
1 Sudan Orange G-a
2 Sudan Orange G-b
3 Sudan Red G
4 Sudan I
5 Sudan II
6 Sudan III
7 Sudan Red 7B
8 Sudan IV
mAU
min0.0 2.5 5.0 7.5 10.0 12.5 15.0
0
1
2
3 4
5 6
78
Curry powder+Sudan dyes
Curry powder
5
10
15
20
As
Cd
Cr
Cu
Fe
Ni
Pb
Se
Zn
7.07
0.312
1.74
15.5
3.17
1.17
0.392
3.36
50.3
6.83
0.312
1.74
15.6
3.18
1.18
0.439
4.46
51.9
6.80 ± 0.64
0.306 ± 0.015
1.87 ± 0.16
15.9 ± 0.9
341 ± 27
1.36 ± 0.22
0.416 ± 0.053
3.56 ± 0.34
52.2 ± 3.2
Element Measured Value(1) (mg/kg)
Measured Value(2) (mg/kg)
Fish CRM DORM-4
Certified Value(mg/kg)
0.0 2.5 5.0 7.5 Conc.0.0
2.5
5.0
7.5
0.0 2.5 5.0 7.5 Conc.0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
Area (x100,000)Area (x10,000)
DCD (85.1>68.1)R2 = 0.997
Melamine (127.1>85.1)R2 = 0.999
2.00 2.25 2.50 2.75 min
0.0
1.0
2.0
3.0
4.0
5.0
6.0
(x100)
5.5 6.0 6.5 min0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5(x1,000)
DCD (85.1>68.1)
Melamine (127.1>85.1)
Cd 214.438nm Standards Standards
Samples Samples
Cr 205.552nm
Pb
Abs Abs
Cd
0
STD 1 µg/L
Samplew/Spike Sample
w/Spike
Samplew/Spike Sample
w/Spike
Samplew/o Spike
Samplew/o SpikeSample
w/o Spike
Samplew/o Spike
STD 10 µg/L
3sec 0 3sec
10 11
Analysis of Food Additives
Shimadzu’s Total Support for Food SafetyAnalytical and Testing Instruments for Food Safety
A wide variety of additives are used in foods such as preservatives, flavor enhancers, coloring agents and antioxidants.
Strict monitoring of food stuffs is essential to detect the use of prohibited chemicals. Each country assigns a unique number or
code of approved chemicals for that country. Shimadzu offers a wide range of products for detecting the prohibited
chemicals, whether they were added intentionally or through contamination.
Analysis of Toxic Metals
Toxic metals (arsenic, lead, cadmium, etc.) in foods must be controlled down to low concentration levels.
Shimadzu atomic absorption spectrophotometers and ICP emission spectrometers offer highly sensitive analysis of trace
elements in foods. Shimadzu’s product lineup also includes X-ray fluorescence spectrometers that can perform elemental
analysis without sample pretreatment. These instruments are ideal for screening analysis and emergency analysis.
Melamine was found to be used as a protein-rich adulterant
in pet food in 2007, and then in infant formula in 2008 in
China. The outbreak of the melamine scandal that killed
many dogs and cats as well as led to the deaths of six infants
and illness of many others caused panic and great concerns
worldwide. Recently, low levels of dicyandiamide (DCD)
residues were found in milk products from New Zealand.
Instead of being added directly as an adulterant, the trace
DCD found in milk products was explained to be relating to
the grass “contaminated by DCD”. Dicyandiamide has been
used to promote the growth of pastures for cows grazing.
Analysis of Dicyandiamide and Melamine in Milk Powders (LC-MS/MS) Analysis of Cd and Pb in Food Additives (AA) Atomic Absorption Spectrophotometer AA-7000
ICP Emission Spectrometer ICPE-9800 Series
Analysis of Preservatives in Food (UV)
UV-VIS Spectrophotometer UV-1800
Calibration curves of DCD and melamine Peak profiles of Cd and Pb
Quantitative results of fish CRM DORM-4
Fish Protein Certified Reference Material for Trace Metals (DORM-4) was used as fish sample.Peak profiles of Cd and Cr
Measurement results of Cd and Pb in α-cyclodextrin
Measured value
Spike and recovery rate
Element Cd
<0.003 µg/g
105 %
Pb
<0.07 µg/g
99 %
Sodium Nitrite in Meat Sorbic Acid in a Processed White Fish Product
Sudan Dyes in Curry Powder
Overlapping of six MRM peaks of 0.5 ng/mL DCD and melamine in milk powder matrix
Below are examples of the analysis of food samples spiked with preservatives.
Analysis of Sudan Dyes in Curry Powder (LC)
Sudan dyes are oil-soluble synthetic dyes used in industrial products. Their use is not authorized in food products in some countries. This is an example of LC analysis of Sudan dyes in curry powder.
Analysis of cadmium (Cd) and lead (Pb) in α-cyclodextrin
(cyclic oligosaccharide), a substance used in functional foods,
pharmaceuticals, cosmetics, etc. The analysis was conducted
by the AA-7000 atomic absorption spectrophotometer.
Analysis of Trace Element Contamination in Fish Sample (ICP)
Fish Protein Certified Reference Material for Trace Metals
(DORM-4) was used as fish sample. The Shimadzu ICPE-9800
Series can provide a rapid method to simultaneously analyze
trace elements in fish. The results show excellent correlation
with the certified reference material.
Features
High resolution
1 nm resolution provides for high data
reliability.
Enhanced security
Functions can be restricted according to
user level.
Instrument validation
Automatic/semi-automatic validation of
wavelength accuracy and wavelength
reproducibility.
Features
Measures from trace levels to high concentrations
Automatic switching between flame and furnace measurements. A
single instrument handles analysis from ppb to ppm levels.
Sophisticated automation
Automation functions enhance analysis efficiency. For instance,
automatic searching sets the optimal gas flow rate and burner
height for flame measurements. Also enables automatic dilution
and re-measurement for over ranged samples compared to the
calibration curve. The autosampler allows automatic addition of
spikes, matrix matching, and dilutions (300x) with true mixing
capability.
Advanced safety technology
World's first AA with vibration sensor to automatically extinguish
the flame in case of an earthquake. Automatic gas-leak checks and
safety functions prevent misuse of the wrong burner head.
Features
Simultaneous multi-element quantitative analysis
Multiple elements can be analyzed simultaneously.
High sensitivity and wide dynamic range
Simultaneous analysis of trace components and major components
Low running costs
Shimadzu's unique mini torch halves argon gas consumption.
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.5µg
0.4µg
0.3µg
0.2µg
0.1µg
nm
Ab
s.
Sample
450 500 550 600 650
0.7
0.6
0.5
0.4
0.3
0.2
0.1
nm
8µg
6µg
4µg
2µg
Ab
s.
250 300 350
Peaks
1 Sudan Orange G-a
2 Sudan Orange G-b
3 Sudan Red G
4 Sudan I
5 Sudan II
6 Sudan III
7 Sudan Red 7B
8 Sudan IV
mAU
min0.0 2.5 5.0 7.5 10.0 12.5 15.0
0
1
2
3 4
5 6
78
Curry powder+Sudan dyes
Curry powder
5
10
15
20
As
Cd
Cr
Cu
Fe
Ni
Pb
Se
Zn
7.07
0.312
1.74
15.5
3.17
1.17
0.392
3.36
50.3
6.83
0.312
1.74
15.6
3.18
1.18
0.439
4.46
51.9
6.80 ± 0.64
0.306 ± 0.015
1.87 ± 0.16
15.9 ± 0.9
341 ± 27
1.36 ± 0.22
0.416 ± 0.053
3.56 ± 0.34
52.2 ± 3.2
Element Measured Value(1) (mg/kg)
Measured Value(2) (mg/kg)
Fish CRM DORM-4
Certified Value(mg/kg)
0.0 2.5 5.0 7.5 Conc.0.0
2.5
5.0
7.5
0.0 2.5 5.0 7.5 Conc.0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
Area (x100,000)Area (x10,000)
DCD (85.1>68.1)R2 = 0.997
Melamine (127.1>85.1)R2 = 0.999
2.00 2.25 2.50 2.75 min
0.0
1.0
2.0
3.0
4.0
5.0
6.0
(x100)
5.5 6.0 6.5 min0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5(x1,000)
DCD (85.1>68.1)
Melamine (127.1>85.1)
Cd 214.438nm Standards Standards
Samples Samples
Cr 205.552nm
Pb
Abs Abs
Cd
0
STD 1 µg/L
Samplew/Spike Sample
w/Spike
Samplew/Spike Sample
w/Spike
Samplew/o Spike
Samplew/o SpikeSample
w/o Spike
Samplew/o Spike
STD 10 µg/L
3sec 0 3sec
12 13
Quantitativeanalysis results
FeCrNiMoCu
70.06%19.18%9.77%0.51%0.48%
Inspection and Analysis of Foreign Matter
Shimadzu’s Total Support for Food SafetyAnalytical and Testing Instruments for Food Safety
Foreign matter can become mixed with food products during the production process for a variety of reasons. The discovery
and identification of foreign matter and ascertaining its source are essential for maintaining food safety.
Shimadzu’s non-destructive testing and analytical technologies can be used to detect foreign matter that is not externally
visible and analyze for the elemental or molecular components.
Analysis of Inorganic Foreign Matter (EDX) Energy Dispersive X-ray fluorescence SpectrometerEDX-7000/8000
Metal foreign matter on chocolate
Infrared spectra of contaminant and fluororesin
Calibration curve (left) and MRM peaks (right) of acrylamide spiked into potato chips matrix, 1-500 ppb with 50 ppb IS (internal standard) added.
Overlay SIM chromatograms of spiked and unspiked styrene samples
An energy-dispersive X-ray fluorescence spectrometer
(EDXRF) permits the easy and rapid identification of
inorganic foreign matter adhering to, or mixed into, food
products. Below is an example of the qualitative analysis of a
metal fragment on chocolate.
Food Processing
Food processing takes a farmed product and turns it in to an edible, marketable product that may have a longer shelf life.
Examples of these processes are baking, canning, fermenting, grilling, heating, hydrolysis, and roasting. During these
processes, undesirable and even toxic compounds can form. Or cross-contamination can occur from the big eight allergens of
egg, fish, milk, peanuts, shellfish, soy, tree nuts, and wheat to non-allergen foods.
Analysis of Acrylamide in Potato Chips (LC-MS/MS)
Acrylamide was found to form in fried foods like potato chips via the so-called Maillard reaction of amino acid asparagine
and reducing sugar glucose at a higher temperature (120°C). The health risk of acrylamide present in many processing foods
became a concern immediately, because it is known that the compound is a neurotoxin and a potential carcinogen to humans.
Analysis of Styrene Leached from Polystyrene Cups (Headspace-GCMS)
Analysis of Organic Foreign Matter (FTIR) IRTracer-100 FTIR Spectrophotometer
The Fourier transform infrared (FTIR) spectrophotometer
measures the organic foreign matter spectrum and compares
it with library data to identify the foreign matter. The
example below shows the analysis of contamination on a
frozen pizza. Some of the foreign substance was scraped off
the frozen pizza, and the infrared spectrum was measured
using a transmission infrared microscope. The spectrum of
this foreign object was similar to that of a fluororesin.
Features
Large sample chamber
Accepts samples of up W300 x D275 x H100 mm.
Easy operation
Automates complex set-up operations. Even novices can easily
conduct accurate measurements.
Features
High sensitivity
High S/N ratio above 60,000:1. Microscope permits analysis of
foreign matter in the micron size range.
Foreign matter analysis program as standard
Powerful support for foreign matter analysis. Includes more than
300 spectra of recognized foreign matter.
Easy maintenance
Automatic dryer protects the optical system. Eliminates superfluous
maintenance.
Worldwide studies have revealed the negative impacts of
household disposable polystyrene cups on human health and
the environment. Styrene is considered as a possible human
carcinogen by the WHO and International Agency for
Research on Cancer (IARC). Migration of styrene from
polystyrene cups containing beverages has been observed.
Styrene enters into our body through the food we eat,
mimics estrogens in the body, and can therefore disrupt
normal hormonal functions. This could also lead to breast
and prostate cancer.
Overlay of SIM chromatograms for styrene
m/z 104 at linearity levels 1 ppb to 50 ppb in
less than 2.5 minutes
Y= 1.1239X +0.594168R2 = 0.9999
2.2 2.3 2.4 2.5 2.6
0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
2.00
(x1,000,000)
1 ppb
2.5 ppb
5 ppb
10 ppb
20 ppb
50 ppb
min
m/z : 104.00
2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8
0.0
2.5
5.0
7.5
(x100,000)
Spiked
Unspiked
m/z : 104.00
min
contaminant
fluororesin
1
Abs
0.5
-04000 3000 2000 1500 1000 1/cm
12 13
Quantitativeanalysis results
FeCrNiMoCu
70.06%19.18%9.77%0.51%0.48%
Inspection and Analysis of Foreign Matter
Shimadzu’s Total Support for Food SafetyAnalytical and Testing Instruments for Food Safety
Foreign matter can become mixed with food products during the production process for a variety of reasons. The discovery
and identification of foreign matter and ascertaining its source are essential for maintaining food safety.
Shimadzu’s non-destructive testing and analytical technologies can be used to detect foreign matter that is not externally
visible and analyze for the elemental or molecular components.
Analysis of Inorganic Foreign Matter (EDX) Energy Dispersive X-ray fluorescence SpectrometerEDX-7000/8000
Metal foreign matter on chocolate
Infrared spectra of contaminant and fluororesin
Calibration curve (left) and MRM peaks (right) of acrylamide spiked into potato chips matrix, 1-500 ppb with 50 ppb IS (internal standard) added.
Overlay SIM chromatograms of spiked and unspiked styrene samples
An energy-dispersive X-ray fluorescence spectrometer
(EDXRF) permits the easy and rapid identification of
inorganic foreign matter adhering to, or mixed into, food
products. Below is an example of the qualitative analysis of a
metal fragment on chocolate.
Food Processing
Food processing takes a farmed product and turns it in to an edible, marketable product that may have a longer shelf life.
Examples of these processes are baking, canning, fermenting, grilling, heating, hydrolysis, and roasting. During these
processes, undesirable and even toxic compounds can form. Or cross-contamination can occur from the big eight allergens of
egg, fish, milk, peanuts, shellfish, soy, tree nuts, and wheat to non-allergen foods.
Analysis of Acrylamide in Potato Chips (LC-MS/MS)
Acrylamide was found to form in fried foods like potato chips via the so-called Maillard reaction of amino acid asparagine
and reducing sugar glucose at a higher temperature (120°C). The health risk of acrylamide present in many processing foods
became a concern immediately, because it is known that the compound is a neurotoxin and a potential carcinogen to humans.
Analysis of Styrene Leached from Polystyrene Cups (Headspace-GCMS)
Analysis of Organic Foreign Matter (FTIR) IRTracer-100 FTIR Spectrophotometer
The Fourier transform infrared (FTIR) spectrophotometer
measures the organic foreign matter spectrum and compares
it with library data to identify the foreign matter. The
example below shows the analysis of contamination on a
frozen pizza. Some of the foreign substance was scraped off
the frozen pizza, and the infrared spectrum was measured
using a transmission infrared microscope. The spectrum of
this foreign object was similar to that of a fluororesin.
Features
Large sample chamber
Accepts samples of up W300 x D275 x H100 mm.
Easy operation
Automates complex set-up operations. Even novices can easily
conduct accurate measurements.
Features
High sensitivity
High S/N ratio above 60,000:1. Microscope permits analysis of
foreign matter in the micron size range.
Foreign matter analysis program as standard
Powerful support for foreign matter analysis. Includes more than
300 spectra of recognized foreign matter.
Easy maintenance
Automatic dryer protects the optical system. Eliminates superfluous
maintenance.
Worldwide studies have revealed the negative impacts of
household disposable polystyrene cups on human health and
the environment. Styrene is considered as a possible human
carcinogen by the WHO and International Agency for
Research on Cancer (IARC). Migration of styrene from
polystyrene cups containing beverages has been observed.
Styrene enters into our body through the food we eat,
mimics estrogens in the body, and can therefore disrupt
normal hormonal functions. This could also lead to breast
and prostate cancer.
Overlay of SIM chromatograms for styrene
m/z 104 at linearity levels 1 ppb to 50 ppb in
less than 2.5 minutes
Y= 1.1239X +0.594168R2 = 0.9999
2.2 2.3 2.4 2.5 2.6
0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
2.00
(x1,000,000)
1 ppb
2.5 ppb
5 ppb
10 ppb
20 ppb
50 ppb
min
m/z : 104.00
2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8
0.0
2.5
5.0
7.5
(x100,000)
Spiked
Unspiked
m/z : 104.00
min
contaminant
fluororesin
1
Abs
0.5
-04000 3000 2000 1500 1000 1/cm
14 15
Analysis of Flavor & Texture
Shimadzu’s Total Support for Food SafetyAnalytical and Testing Instruments for Food Safety
Analysis of Off-Flavor (Headspace GC-MS) Application System of Headspace-Trap GC-MSGCMS-QP2010 Ultra with HS-20 Trap
Flavor Release Evaluation Kit EZ Test-GC/MS
2,4,6-Trichloroanisole (TCA) is a natural compound known as
the chief cause of musty smell in wine. Below is an analysis
of wine for 1 ng/L of TCA by headspace-trap-GC-MS. The
headspace-trap method is 10 times more sensitive then
headspace alone when coupled to a GC-MS.
Prevention of Defects in Packaging
Adhesive Strength Testing (Strength Tester) EZ Test Universal Testing Machines
A wide range of materials is used for packaging, including
aluminum and other metals and non-metallic materials such
as paper and plastic. According to their application, these
materials must meet certain tensile, compression, and
bending strength requirements, and if an adhesive is used,
adhesive strength requirements. In the examples below,
adhesive strength results for liquid-filled pouch and dry
snack package specimens are shown. In both instances,
the adhesives meet the requirements of being strong enough
to protect the contents, but weak enough so that the
contents can easily be accessed by consumers.
Residual Solvent Analysis (Headspace GC) Gas Chromatograph Headspace Analysis System
Quality control of residual organic solvents is required, as
the manufacture of food packaging materials and containers
involves the use of organic solvents in printing inks and
adhesives. These components can be analyzed by headspace
GC or by diluting in a solvent and directly injecting this
sample solution into a GC instrument.
Testing the strength and functionality of packaging materials is essential to ensure food products reach consumers fresh and
undamaged. To aid the development and quality control testing of packaging materials, Shimadzu analytical and testing
technologies allow the analysis of residual solvents in packaging materials, strength testing of adhesive seals, and checks for
pin holes in packaged products.
Flavor Release Evaluation Kit (EZ Test-GC-MS)
Evaluation of flavor and texture with each “chew” is
possible. The EZ Test measures the texture with each “chew”
as the flavors are released into the sampling bag. The flavors
are extracted from the bag with a SPME sample needle and
injected into the GC-MS for flavor analysis.
SIM Chromatogram of (TCA) in wine measured with HS-Trap GC/MS at 1 ng/L
GCMS-QP2010 Ultra
GCMS flavor analysis EZ Test texture analysis
SPME needle and sampling bag
Liquid-filled pouch
Headspace GC analysis of residual organic solvent dry snack packaging
Dry snack packaging
EZ Test
Features of HS-20 Trap
Multiple modes
Loop Mode captures fixed volume for transfer to GC.
Multiple Headspace Extraction (MHE) captures the sample from the
same vial multiple times, resulting in up to ten GC-MS analyses. Trap
Mode increases sensitivity by a factor of 10 by trapping the
headspace.
Automation
90-position sample tray holds 10 or 20 mL vials. A 12-position
equilibration oven prepares upcoming samples. Optional barcode
reader available.
Features
Evaluation of texture and flavor with each “chew”
Eliminate off-flavor and off-texture of foods.
Research & develop new textures and flavors which vary with each
“bite”.
Features
Comprehensive range of jigs
Diverse strength testing: peeling, tensile, shear, bending,
compression, etc.
Measures brittle and elastic samples
5 kN max. capacity, 920 mm stroke (long-type).
Food texture evaluation
Objective testing which analyzes the consistency and mouth feel of
foods.
Features
High sensitivity
High-sensitivity analysis of volatile components (low-boiling-point
components).
Easy maintenance
No non-volatile components enter the GC.
Reduced analysis time
Faster analysis than direct-injection GC for liquid samples containing
high-boiling-point components.
1-11-21-3
N40
30
20
10
00 5 10 15 20
min
1-11-21-3
N15
12
9
6
3
00 7 14 21 30
min
Sample only
Sample spiked + standard
tolueneMEK
ethylacetate
isopropanol
methanol
Released by textureBrittle
6.0
5.0
4.0
3.0
2.0
1.0
0.08.0 10.0 12.0 14.0 16.0 18.0
14 15
Analysis of Flavor & Texture
Shimadzu’s Total Support for Food SafetyAnalytical and Testing Instruments for Food Safety
Analysis of Off-Flavor (Headspace GC-MS) Application System of Headspace-Trap GC-MSGCMS-QP2010 Ultra with HS-20 Trap
Flavor Release Evaluation Kit EZ Test-GC/MS
2,4,6-Trichloroanisole (TCA) is a natural compound known as
the chief cause of musty smell in wine. Below is an analysis
of wine for 1 ng/L of TCA by headspace-trap-GC-MS. The
headspace-trap method is 10 times more sensitive then
headspace alone when coupled to a GC-MS.
Prevention of Defects in Packaging
Adhesive Strength Testing (Strength Tester) EZ Test Universal Testing Machines
A wide range of materials is used for packaging, including
aluminum and other metals and non-metallic materials such
as paper and plastic. According to their application, these
materials must meet certain tensile, compression, and
bending strength requirements, and if an adhesive is used,
adhesive strength requirements. In the examples below,
adhesive strength results for liquid-filled pouch and dry
snack package specimens are shown. In both instances,
the adhesives meet the requirements of being strong enough
to protect the contents, but weak enough so that the
contents can easily be accessed by consumers.
Residual Solvent Analysis (Headspace GC) Gas Chromatograph Headspace Analysis System
Quality control of residual organic solvents is required, as
the manufacture of food packaging materials and containers
involves the use of organic solvents in printing inks and
adhesives. These components can be analyzed by headspace
GC or by diluting in a solvent and directly injecting this
sample solution into a GC instrument.
Testing the strength and functionality of packaging materials is essential to ensure food products reach consumers fresh and
undamaged. To aid the development and quality control testing of packaging materials, Shimadzu analytical and testing
technologies allow the analysis of residual solvents in packaging materials, strength testing of adhesive seals, and checks for
pin holes in packaged products.
Flavor Release Evaluation Kit (EZ Test-GC-MS)
Evaluation of flavor and texture with each “chew” is
possible. The EZ Test measures the texture with each “chew”
as the flavors are released into the sampling bag. The flavors
are extracted from the bag with a SPME sample needle and
injected into the GC-MS for flavor analysis.
SIM Chromatogram of (TCA) in wine measured with HS-Trap GC/MS at 1 ng/L
GCMS-QP2010 Ultra
GCMS flavor analysis EZ Test texture analysis
SPME needle and sampling bag
Liquid-filled pouch
Headspace GC analysis of residual organic solvent dry snack packaging
Dry snack packaging
EZ Test
Features of HS-20 Trap
Multiple modes
Loop Mode captures fixed volume for transfer to GC.
Multiple Headspace Extraction (MHE) captures the sample from the
same vial multiple times, resulting in up to ten GC-MS analyses. Trap
Mode increases sensitivity by a factor of 10 by trapping the
headspace.
Automation
90-position sample tray holds 10 or 20 mL vials. A 12-position
equilibration oven prepares upcoming samples. Optional barcode
reader available.
Features
Evaluation of texture and flavor with each “chew”
Eliminate off-flavor and off-texture of foods.
Research & develop new textures and flavors which vary with each
“bite”.
Features
Comprehensive range of jigs
Diverse strength testing: peeling, tensile, shear, bending,
compression, etc.
Measures brittle and elastic samples
5 kN max. capacity, 920 mm stroke (long-type).
Food texture evaluation
Objective testing which analyzes the consistency and mouth feel of
foods.
Features
High sensitivity
High-sensitivity analysis of volatile components (low-boiling-point
components).
Easy maintenance
No non-volatile components enter the GC.
Reduced analysis time
Faster analysis than direct-injection GC for liquid samples containing
high-boiling-point components.
1-11-21-3
N40
30
20
10
00 5 10 15 20
min
1-11-21-3
N15
12
9
6
3
00 7 14 21 30
min
Sample only
Sample spiked + standard
tolueneMEK
ethylacetate
isopropanol
methanol
Released by textureBrittle
6.0
5.0
4.0
3.0
2.0
1.0
0.08.0 10.0 12.0 14.0 16.0 18.0
Shimadzu’s Total Support for Food Safety
Printed in Japan 3655-10401-50AIT
Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its affiliates, whether or not they are used with trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.
For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.
© Shimadzu Corporation, 2014www.shimadzu.com/an/
C10G-E020B
Analytical and Testing Instruments for Food Safety
Shimadzu’s Total Supportfor Food Safety
List of Analytical and Testing Instruments for Food Safety
Shimadzu Balances
UniBloc family of balances
MALDI-TOF Mass Spectrometer (MALDI-TOF-MS)
Gas Chromatograph (GC)
UV-VIS Spectrophotometer(UV)
FTIR Spectrophotometer(FTIR)
Liquid Chromatograph (LC)
Gas Chromatograph Mass spectrometer (GC-MS)
Liquid Chromatograph Mass spectrometer (LC-MS)
Atomic Absorption Spectrophotometer (AA)
ICP Emission / Spectrometer (ICP, ICP-MS)
X-Ray Fluorescence Spectrometer (XRF, EDX)
Universal Testing Machine (AG, EZ Test)
ResidualPesticides
MycotoxinsVeterinary
PharmaceuticalFood
AdditiveToxic
Metals
Inspection andAnalysis of
Foreign MatterOff-Flavor
Prevention ofDefects inPackaging
Region andType
Identification
Identificationand Observation
of Bacteria