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Semester I Exam Review 2014
Chapters 1-4
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Which is the sugar component of a DNA nucleotide?
A. Deoxyribose B. Adenine C. Glucose D. Phosphate
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A. Deoxyribose
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Which substances are components of a DNA nucleotide?
A. Ribose, phosphate, and uracil B. Phosphate, ribose, and adenine C. Phosphate, deoxyribose, and
uracil D. Thymine, deoxyribose, and
phosphate
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D. Thymine, deoxyribose, and phosphate
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The individuality of an organism is determined by the organism’s
Nitrogenous bases Transfer RNA molecules DNA nucleotide sequence Amino acids
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DNA nucleotide sequence
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Which two bases are present in equal amounts in a double-stranded DNA molecule? Cytosine and thymine Adenine and thymine Adenine and uracil Cytosine and uracil
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Adenine and thymine
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During DNA replication, which enzyme is responsible for “unzipping” the DNA molecule?
DNA polymerase DNA restriction enzymes DNA ligase DNA helicase
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DNA helicase
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A human DNA segment was inserted into a bacterial cell and became incorporated into the bacterial DNA. This technique is an example of Cloning Genetic counseling Artificial selection Genetic engineering
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Genetic engineering
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Which technique is used to alter bacteria in such a way that they produce human insulin?
Hydrolysis DNA replication Recombinant DNA formation Genetic screening
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Recombinant DNA formation
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The insertion of a human DNA fragment into a bacterial cell might make it possible for
The bacterial cell to produce a human protein
The cloning of the human that donated that DNA fragment
Humans to become immune to an infection by this type of bacteria
The cloning of this type of bacteria
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The bacterial cell to produce a human protein
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Many diabetics are now using insulin that was made by certain bacteria. The ability of these bacteria to produce insulin was most likely the result of Using radiation to trigger mutations Genetic mapping of bacterial DNA to
activate the gene for insulin production Deleting many DNA segments from
bacterial DNA Inserting a portion of human DNA into
the ring-shaped DNA of bacteria
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Inserting a portion of human DNA into the ring-shaped DNA of bacteria
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A small amount of DNA was taken from a fossil of a mammoth found frozen in glacial ice. Genetic technology can be used to produce a large quantity of identical DNA from this mammoth’s DNA. In this technology, the original DNA sample is used to
a. stimulate differentiation in other mammoth cells.B. Act as a template for repeated replicationc. Provide fragments to replace certain human body chemicals.D. Trigger mitosis to obtain new base sequences
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Act as a template for repeated replication
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Scientists have cloned sheep, but not a human. The best explanation for this situation is that
There are many ethical problems involved in cloning humans
The technology to clone humans has not been explored
Human reproduction is very different from that of other mammals
Cloning humans would take too long.
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What is the genetically engineered curdling agent in the cheese lab?
Rennen Chymosin Buttermilk Whole Milk
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Chymosin
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Why did scientists produce recombinant rennin? 1. Produce large quantities. 2. Cheaper.
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Biuret solution is an indicator for what substance? Starch Glucose Fats Proteins
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Proteins
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What color does iodine turn in the presence of starch? Rusty yellow. Clear Purple/blue Yellow/orange
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Tomato plants in a garden are not growing well. The gardener hypothesizes that the soil is too acidic. To test this hypothesis accurately, the gardener could Change the pH of the soil Plant seeds of a different kind of
plant Move the tomato plants to an area
with less sunlight Reduce the amount of water
available to the plant
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Change the pH of the soil
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To locate a specimen on a prepared slide, a student should begin with the low-power objective rather that the high-power objective because the Field of vision is smaller under low power than under
high power Field of vision is larger under low power than under
high power Specimen does not need to be stained for
observation under low power but must be stained for observation under high power
Portion of the specimen that can be observed under low power is less than the portion that can be observed under high power.
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Field of vision is larger under low power than under high power
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To measure 50 mL, which tool would be the most accurate? Micropipet Pipet graduated cylinder Balance Microcentrifuge tube
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Graduated cylinder
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5 kg is equal to _______g
500 5000 0.005 0.0005
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5000
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Which group of measurements contains only metric units?
5 in, 3 kg, 40 oz
5 mm, 3 g, 40 lb
5 in, 3 lb, 40 mL
5 mm, 3 g, 40 mL
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5 mm, 3 g, 40 mL
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Which objective of a compound microscope would show the largest field of view? 20x 5x 10x 44x
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5x
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Proteins are synthesized from less complex organic compounds known as:
Carbons Starches Amino acids enzymes
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Amino acids
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In what organelle is DNA found? Cytoplasm Nucleus Endoplasmic reticulum Lysosome
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nucleus
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Which enzyme “pastes” rDNA fragments together? Transformation enzyme Restriction enzyme DNA ligase DNA polymerase Nuclease enzyme
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DNA ligase
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A product that has entered the product pipeline Is ready for clinical testing to begin. Is being produced on a large scale by
the manufacturing department Has been determined safe and
effective Needs to be reviewed regularly with
a comprehensive product development plan.
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Needs to be reviewed regularly with a comprehensive product development plan
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Biotechnology research labs are commonly found at all of the following facilities except Universities Medical clinics Companies Government agencies
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Medical clinics
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The primary goal in a biotechnology company is to Engage in “pure science” Cure all the diseases of the world Publish results in scientific journals Provide a product of service that is
useful to society and results in earnings
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Provide a product of service that is useful to society and results in earnings
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What is meant by cell specialization? Different cell lines (CHO & HeLa cells) are
more suited to producing certain kinds of recombinant proteins.
Prokaryotic cells specialize in certain tasks and can therefore grow more rapidly than eukaryotic cells.
There is great cell variety in multicellular organisms in which different kinds of cells have different jobs to do.
All of the above
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There is great cell variety in multicellular organisms in which different kinds of cells have different jobs to do
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Which of the following is an expression of concentration units?
µg/µL
mg/mL
µM
All of these
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all
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As a solution becomes dilute The proportion of solute to solvent
increases The proportion of solvent to solute
increases The normality of the solution shifts Re-ionization begins to occur
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The proportion of solvent to solute increase
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Nucleotides are bonded to each other on a nucleic acid by
Hydrogen bonds Antiparallel bonds Peptide bonds Phosphodiester bonds
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Phosphodiester bonds
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A circular plasmid was cut with a restriction enzyme & three bands were visualized after electrophoresis. How many cuts were made in the plasmid?
1 2 3 4
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3
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Which agarose concentration would work best for separating very large molecules? 3% 2% 0.8% All choices would work
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.08%
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During a DNA extraction, the purpose of smashing the fruit was to Break the phosphodiester bonds
between nucleotides Break open the nuclear membrane Break apart the cellulose in the plant
cells. Break apart the cell membrane.
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Break apart the cellulose surrounding plant cells.
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The types of cells commonly used in biotechnology are Bacteria Yeast Chinese hamster ovary cells All of the above
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all
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Review micropipettor settings P-1000 P-200 P-20
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Metric conversions
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After an enzyme cuts DNA into smaller pieces, this enzyme is responsible for gluing the new DNA pieces together
DNA ligase DNA helicase DNA emporase Restriction enzymes
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DNA ligase
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What is the total magnification of an object if you are using 10X oculars and 4X objective?
14X 400X 40X 4X
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40X
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In gel electrophoresis, what makes the DNA migrate through the gel during electrophoresis?
Water Gravity Salt Electricity
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Electricity
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In PCR, the purpose for the heating cycle is to
Kill the bacteria. Make the DNA glow. Separate the DNA strands. Move the DNA in the gel.
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Separate the DNA strands
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Antiparallel, with regard to DNA means that:
The strands cross each other The strands run parallel to each
other but run in opposite directions The strands run the same directions
and are parallel Strands run at right angles to each
other
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The strands run parallel to each other but run in opposite directions
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EcoRI is an example of which type of enzyme? Restriction endonuclease RNA polymerase DNA polymerase DNA ligase
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Restriction endonuclease
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