Download - Review of Bacterial Infection Specimen
-
8/2/2019 Review of Bacterial Infection Specimen
1/45
Review of Bacterial infection
specimenFor OSCE examination
-
8/2/2019 Review of Bacterial Infection Specimen
2/45
To Find more Mycobacterium TB
S. aureus
Klebsiella
Cell wall differences - +ve, -ve, Mycolicacid
AFB
MAFB
Burkhloderia pseudomellei
-
8/2/2019 Review of Bacterial Infection Specimen
3/45
Gram Stain
-
8/2/2019 Review of Bacterial Infection Specimen
4/45
Bacterial Cell Wall
-
8/2/2019 Review of Bacterial Infection Specimen
5/45
-
8/2/2019 Review of Bacterial Infection Specimen
6/45
-
8/2/2019 Review of Bacterial Infection Specimen
7/45
The cell wall of a bacteria(1) is found externally to the plasma membrane.(2) is semirigid.(3) protecting bacteria against osmotic lysis.
(4) consists of peptidoglycan.
-
8/2/2019 Review of Bacterial Infection Specimen
8/45
Lactobacillusspp..
-
8/2/2019 Review of Bacterial Infection Specimen
9/45
Gram positive bacilli
-
8/2/2019 Review of Bacterial Infection Specimen
10/45
Typical Gram stains
Gram-positive bacilli:
Thick: Clostridium
Thin : Listeria.
Branched:Nocardia,
Actinomycetes
-
8/2/2019 Review of Bacterial Infection Specimen
11/45
Clostridium perfringens
No surrounded RBC (Cell lysis)
-
8/2/2019 Review of Bacterial Infection Specimen
12/45
Clostridiumwith subterminal spores
Susan D. Caston
-
8/2/2019 Review of Bacterial Infection Specimen
13/45
Diphtheroids (Propionibacterium andCorynebacterium spp)
Susan D. Caston
The orientation of the bacteria in the clumps, at variable andrandom angles, sometimes called "Chinese letters" Many other
bacteria also have this appearance, includingRothia spp,
-
8/2/2019 Review of Bacterial Infection Specimen
14/45
Listeria monocytogenes
-
8/2/2019 Review of Bacterial Infection Specimen
15/45
Lactobacillus
Susan D. Caston
Chains of Gram-variable, but mostly Gram-positive, non-
sporing, regular small bacilli. This is characteristic of
Lactobacillus, especially in genital tract specimens.
-
8/2/2019 Review of Bacterial Infection Specimen
16/45
Nocardia
-
8/2/2019 Review of Bacterial Infection Specimen
17/45
-
8/2/2019 Review of Bacterial Infection Specimen
18/45
Actinomyces
Susan D. Caston
-
8/2/2019 Review of Bacterial Infection Specimen
19/45
Mycobacterium
Susan D Coston
Mycobacterium species ,especially the rapidly growing
mycobacteria, such asM. fortuitum andM. chelonae can
sometimes be seen in Gram stains as beaded gram-positive=
-
8/2/2019 Review of Bacterial Infection Specimen
20/45
Mycobacterium marinum "ghosts"
Susan D Caston
-
8/2/2019 Review of Bacterial Infection Specimen
21/45
Hyphae
Susan D Coston
-
8/2/2019 Review of Bacterial Infection Specimen
22/45
Bacillus anthracis in CSF
Jernigan JA: EID 2001;7:933-944
-
8/2/2019 Review of Bacterial Infection Specimen
23/45
Bacillus cereus
http://www-micro.msb.le.ac.uk/video/Bcereus.mov -
8/2/2019 Review of Bacterial Infection Specimen
24/45
Gram positive cocci
-
8/2/2019 Review of Bacterial Infection Specimen
25/45
Typical Gram stains
Gram-positive cocci:
Cluster: Staph. Aureus
Tetrad: Micrococcus spp.
Chain: Streptococcus
-
8/2/2019 Review of Bacterial Infection Specimen
26/45
Staph. Vs. Pneumococcus
-
8/2/2019 Review of Bacterial Infection Specimen
27/45
Pneumococcus
Lancet-shaped,
diplococci withcapsule
-
8/2/2019 Review of Bacterial Infection Specimen
28/45
S.pneumoniae
-
8/2/2019 Review of Bacterial Infection Specimen
29/45
Streptococcus
Susan D. Caston
-
8/2/2019 Review of Bacterial Infection Specimen
30/45
Viridans Streptococcus
Susan D. Caston
Usually forms short to long chains. The individual cells are
often elongated.
-
8/2/2019 Review of Bacterial Infection Specimen
31/45
Streptococcus pyogenes
-
8/2/2019 Review of Bacterial Infection Specimen
32/45
Gram negative cocci
T i l G t i
-
8/2/2019 Review of Bacterial Infection Specimen
33/45
Typical Gram stains
Gram-negative cocci :
Diplococci: usually characteristic ofNeiseria spp., such as
N gonorrhea orN meningitides. In addition,Moraxella spp.
andAcinetobacter spp.are often diplococcal in morphology.
Acinetobacter can be pleomorphic, and sometimes appear
as Gram-positive cocci.
-
8/2/2019 Review of Bacterial Infection Specimen
34/45
N. gonorrheae Acinetobacter spp.
Acinetobacter- A major characteristic of this species is itsgram stain morphology: they appear as gram-negative
coccobacilli but are frequently confused with gram-
negative diplococci characteristic ofNeisseriaspp.
Susan D. Caston
Acinetobacter spp
-
8/2/2019 Review of Bacterial Infection Specimen
35/45
Acinetobacter spp.
Acinetobacter often stains as Gram-variable cocci or coccobacilli. It
is not unusual for this bacterium to stain as a Gram-positive coccus
in blood cultures positive for the bacterium. Ifflattening of the"cocci" at their apposing surfaces is observed, then acinteobacter
should be high on the list. Too short a period of destaining with
acetone-alcohol can also present this picture, but in this case the red
color of the proteinaceous background suggests that this was not the
case here Susan D. Caston
-
8/2/2019 Review of Bacterial Infection Specimen
36/45
Acinetobacter spp.
Susan D. Caston
-
8/2/2019 Review of Bacterial Infection Specimen
37/45
Gram negative bacilli
-
8/2/2019 Review of Bacterial Infection Specimen
38/45
Typical Gram stains
Gram-negative coccobacilli :H influenzae
-
8/2/2019 Review of Bacterial Infection Specimen
39/45
Typical Gram stains
Gram-negative bacilli :
Thin rods:E coli
-
8/2/2019 Review of Bacterial Infection Specimen
40/45
An advancing colony of Klebsiella bacteria is halted when itencounters an antibiotic.
Source: : Gary Carlson,
www.gcarlson.com/microbial_krebsiella.htm
http://www.gcarlson.com/microbial_krebsiella.htmhttp://www.gcarlson.com/microbial_krebsiella.htm -
8/2/2019 Review of Bacterial Infection Specimen
41/45
Typical Gram stains
Gram-negative bacilli :
Curved rods: Vibrio
Campylobacter
Thin needle shape:
Fusobacterium
-
8/2/2019 Review of Bacterial Infection Specimen
42/45
Seagull-wings
appearance
Campylobacter
Susan D. Caston
-
8/2/2019 Review of Bacterial Infection Specimen
43/45
Fusobacterium
Susan D. Caston
The needle-like tapering ends of the bacilli are specific forthis species. The only other commonly encountered
Fusobacterium species,F. necrophorum, does not possess
the tapering ends and tends to be much more pleomorphic.
-
8/2/2019 Review of Bacterial Infection Specimen
44/45
Candida
Susan D Caston
-
8/2/2019 Review of Bacterial Infection Specimen
45/45
Cryptococcus neoformans
Susan D Caston
\Cryptococcus neoformans in spinal fluid. These yeast can
be easily mistaken for blood monocytes, especially when
they are present in low numbers.