Download - Quantum Dot Overview
-
8/14/2019 Quantum Dot Overview
1/22
An overview of quantumquantumdotdotsynthesis, applications
and safetyNastassja Lewinski
Department of Bioengineering
-
8/14/2019 Quantum Dot Overview
2/22
What are Quantum Dots?
II-VI: ZnS, ZnSe, CdSe,
CdTe, CdS, HgS
III-V: GaAs, GaN, InP,
InAs, InGaAs
IV-VI: PbS, PbSe
II
IV V VIIII
-
8/14/2019 Quantum Dot Overview
3/22
What do Quantum Dots look like?
Control AntiHER2
-
8/14/2019 Quantum Dot Overview
4/22
Quantum dots
~2 - 6 nm
-
8/14/2019 Quantum Dot Overview
5/22
How are they made?
-
8/14/2019 Quantum Dot Overview
6/22
Synthesis Movie
http://www.youtube.com/watch?v=2XfFQKEfCcw&feature=related
-
8/14/2019 Quantum Dot Overview
7/22
Why do we care?
Medintz et al. Nature Mat., 4, 2005.
-
8/14/2019 Quantum Dot Overview
8/22
Luminescence
Energy
Ground State
Excited State
Ab
sorp
tion
(exc
ita
tion
)
F
luorescence
(em
iss
ion
)
-
8/14/2019 Quantum Dot Overview
9/22
Electromagnetic Spectrum
400 nm 700 nm
-
8/14/2019 Quantum Dot Overview
10/22
Bare CdSe QD Spectra
-
8/14/2019 Quantum Dot Overview
11/22
CdSe/ZnS Excitation-Emission Matrix
-
8/14/2019 Quantum Dot Overview
12/22
-(CH-CH-CH2-CH)m-
O
OHO NH
(O-CH2-CH2)n-OCH3
Water solubilization
Silica shelling
Polymaleic anhydride oxide - polyethylene glycol (PMAO-PEG)
HS
O
OH
-[CH2-CH]x-[CH2-CH]y-[CH2-C-CH2-C-CH2-C]z-
COOC4H9COOC2H5
COOH CH3
CONHC8H17
COOH
Amphiphilic triblock polymer
Mercaptoundecanoic acid
O
Si SH
O
3-mercaptopropylmethyldimethoxysilane
(MPS)
O
Si O
O
O
O
nO
Si NH2
O2-[methoxy(polyethleneoxy)propyl]-
trimethoxysilane (PEOS)
3-aminopropylmethyldimethoxysilane
(APS)
Polymer wrapping
Ligand exchange
-
8/14/2019 Quantum Dot Overview
13/22
Particle Aggregation
-
8/14/2019 Quantum Dot Overview
14/22
How are they used?
Michalet et al. Science, 307, 2005
Gao et al. Nat.Biotech., 22, 2004Green, Angew. Chem., 43, 2004
Sapsford et al. Sensors, 6, 2006
Parungo et al. JTCS, 129, 2005
-
8/14/2019 Quantum Dot Overview
15/22
Tumor Tissue Margin Detection
-
8/14/2019 Quantum Dot Overview
16/22
NIR Optical Window
Absorptioncoefficient(cm
-1)
Wavelength (nm)
-
8/14/2019 Quantum Dot Overview
17/22
Toxicity Concerns
HS
O
OH
-(CH-CH-CH2-CH)m-
O
OH
O NH
(O-CH2-CH2)n-OCH3
Low pH - lysosomes
Oxidation - peroxisomes
-
8/14/2019 Quantum Dot Overview
18/22
-
8/14/2019 Quantum Dot Overview
19/22
Lewinski et al. Small, 4, 2008
-
8/14/2019 Quantum Dot Overview
20/22
Daphnia magna
4x Brightfield
-
8/14/2019 Quantum Dot Overview
21/22
Qdot Localization
10x Brightfield
10x Fluorescence
(DAPI excitation filter)
Rectum
-
8/14/2019 Quantum Dot Overview
22/22
Take Home