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Production of proteins from clonedgenes
Terms:Expression vectorsFusion proteinsStrong/weak promotersGlycosylation patternCell-free translation
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Cloning for protein produktion
Fig 13.1 p226
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Production of eukaryotic proteins in E.coli
Fig 13.2 p227
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Signals for gene expression
Fig 13.3 p228
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Typical Promoter sequences
Fig 13.4 p228
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Expression vector for expression of foreign genes in E.coli
Fig 13.5 p229
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Strong/weak promoters
Fig 13.6 p230
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Gene regulation
Fig 13.7 p230
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Promoters in expression vectors
Fig 13.8 p231
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Cassette vector
Fig 13.9 p232
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Translation of fusion protein
Fig 13.10 p233
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Problem with secondary structureat the start of transcription
Fig 13.11 p233
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Affinity chromatografy for purification of Glutation-S-transferas
Fig 13.12 p234
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Cleavage of a fusion protein
Fig 13.13 p234
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Problem with expression of foreignproteins in E.coli
Fig 13.14 p235
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Inclusion bodies
Fig 13.15 p236
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Promoters for eukaryoticexpression vectors
Fig 13.16 p238
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Glycosylation pattern
Fig 13.17 p239
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Inclusion bodies in insect cells
Fig 13.18 p240
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Cell free translation system
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Cell-free translation
Figure 11.18p200