Download - Presentación leandro vetcher
Green Pacific BiologicalsJunio 2012
Leandro Vetcher – CEO
[email protected]@lvetcher
Green Pacific Biologicals - Not for distribution
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More efficient engineering
Stable, stronger gene expression
Access to the entire cell
Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution
GPB: Plataforma de ingenieria genetica
Only company with rapid and stable eukaryotic algae nuclear genetic engineering
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GPB: Soluciones para biocombustibles
Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution
Expresión génica nuclear mas fuerte
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WT GPBGFP protein
stds (ng)Cell extract
Wild
-typ
eG
PB S
trai
ns
GFP expression by Western blotting Accumulation of GFP by confocal microscopy
Expresión génica nuclear estable
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Avg.
fluo
resc
ence
pe
r cel
l (au
)
Avg.
fluo
resc
ence
pe
r cel
l (au
)
Mas transformantes expresando transgenes
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More rapid and efficientengineering
Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution
RNAi funcional- ingeniería metabólica
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Genetic RNAi using GPB platform better than traditional mutant
Starch lower Oil higher Growth normal
Membrane part+GFP Lipid droplet part+GFPUntransformed cell
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Localización subcelular de proteínaMembrane localization
Lipid droplet localization
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Secreción de aceites no-toxica Non-toxic mechanism Secreted LDs spontaneously separates, sidestepping
harvesting/extraction Works by interactions between proteins on LDs and cell membrane
or other cellular components Platform makes GPB uniquely positioned to implement oil secretion
in algae
BASE CASE:• Milk harvested by killing cow
• Majority of energy Making cow
GPB CASE:• Milk harvested through secretion and cow returned to pasture
• Majority of energy (grass) goes into making milk
• If cows are milked daily, there is no benefit to having cows that can store more than one day’s worth of milk
?
+MILK MILK
Beneficios de secreción de aceites no-toxica
Modelado Tecno-económico
• Physics: energy balanced, reasonable energy inputs•Biology: realistic growth and enzymatic rates, cell densities, PSE• Interaction between variables (e.g., cell density affects total energy captured)• Pilot and commercial capex and operational cost for PBRs and open ponds (with Tryg Lundquist, PhD at Cal Poly)
Oil productivity model + operational & capital costs
Modelado Tecno-económico: valor de GPBSecretion• Enables continuous process through recycling of cells after secretion• Most benefit through higher TAG Productivity • Elimination of several costly process steps in TAG recovery
Oil metabolic engineering• Improved redirection of light energy into TAGs • Very high oil content levels not necessary with oil secretion
Other • Identify additional bioengineering solutions• Prioritize R&D projects based on synergies and on relative impact in cost
ValidationWe can reach $50/bbl with realistic performance metrics for each of our genetic engineering solutions
Process Flow Diagram Algae Biofuel Facility
Flash Dryer
High Rate Algae Ponds
Inoculation Ponds
Settling BasinGravity
Thickener
Solar Drying Beds
Anaerobic Digester
Generator
Make-up Water
Recirculation
Algae Biomass
Algae Biomass
Algae BiomassResiduals
Solvent Based Oil-Extraction
Unrefined Oil
CH4
+
CO2
CO2Digestate
Natural Gas
Make-up Water
Blowdown
Base Case GPB Process
Work with Tryg Lundquist, Assistant Professor at Cal Poly
Algae acts a catalyst that converts CO2 and sunlight into TAGs efficiently, without being consumed in the process
0 5 10 15 20 25 30 35 40 45 50 55 600
200
400
Base Case 0%50% 90%
% Oil (w/w)Cost
per
bar
rel (
$/bb
l)
Early adoption of tecnoeconomic analysis provides: Quantification of value of biofuel solutions Guidance of R&D projects Validation
Modelado Tecno-económico