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O
O
N
O
CH3O
OCH3O
OCH3OCH3
OH
OH
N
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Why are some plants poisonous ?
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Why are some plants poisonous ?
Herbivory
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Why are some plants poisonous ?
A. Herbivory
B. How do plants protect themselvesagainst herbivory?
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1. Physical Protection
(spines, thorns, leaf hairs)
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1. Physical Protection(spines, thorns, leaf hairs)
2. Chemical protection that affects taste,smell or other physiological damage.
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Poison – any substance, particularly chemical, that causes injury, illness or death.
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Poison – any substance, particularly chemical, that causes injury, illness or death.
“All substances are poisonous; there is none which is not a poison. The right dose differentiates a poison and a remedy”
Paracelsus (1493 – 1541)
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Poison – any substance, particularly chemical, that causes injury, illness or death.
“All substances are poisonous; there is none which is not a poison. The right dose differentiates a poison and a remedy”
Paracelsus (1493 – 1541)
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Toxicant
Xenobiotic
Natural toxin
Phytotoxin
Mycotoxin
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“All substances are poisons; there is none which is not a poison. The right dose differentiates a poison and a remedy”
Paracelsus (1493 – 1541)
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“All substances are poisons; there is none which is not a poison. The right dose differentiates a poison and a remedy”
Paracelsus (1493 – 1541)
1. What ?
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“All substances are poisons; there is none which is not a poison. The right dose differentiates a poison and a remedy”
Paracelsus (1493 – 1541)
1. What ?
2. How Much ?
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Classes of Toxins
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Classes of Toxins
Alkaloids
N
OCH2 O
O
O
HO CH2OH
N
OH
OH
HHO
N
N
N
N
HO
O
H
N
OH
OH
OCH3
OCH3
CH3OO
CH3O
O
N
O
O
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Classes of Toxins
Alkaloids
N
OCH2 O
O
O
HO CH2OH
N
OH
OH
HHO
N
N
N
N
HO
O
H
N
OH
OH
OCH3
OCH3
CH3OO
CH3O
O
N
O
O
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Classes of Toxins
Alkaloids
N
OCH2 O
O
O
HO CH2OH
N
OH
OH
HHO
N
N
N
N
OH
OH
OCH3
OCH3
CH3OO
CH3O
O
N
O
O
N
HO
O
H
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Metal-binding Compounds
OH
OH
O
O
Ca2+
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Metal-binding Compounds
O
O
O
OCa
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Phenolic compounds
OH
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Phenolic compounds
HO
HO
OHHO O
OH
OH
OHOHO
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Sesquiterpene Lactones
OO
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B. Physiological Effects
hepatotoxins
neurotoxins
myotoxins
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Identification of Plant Toxins
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Identification of Plant Toxins
A. Need an appropriate biological assay for testing toxicity.
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Identification of Plant Toxins
A. Need an appropriate biological assay for testing toxicity.
1. Cell, enzyme, chemical based assay.
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Identification of Plant Toxins
A. Need an appropriate biological assay for testing toxicity.
1. Cell, enzyme, chemical based assay.
2. Small Animal Assay (mouse, rat).
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Identification of Plant Toxins
A. Need an appropriate biological assay for testing toxicity.
1. Cell, enzyme, chemical based assay.
2. Small Animal Assay (mouse, rat).
3. Large Animal Assay (sheep, cow).
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B. Bioassay guided chemical fractionation.
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B. Bioassay guided chemical fractionation.
1. Chemical Extraction of Plant material.
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B. Bioassay guided chemical fractionation.
1. Chemical Extraction of Plant material.2. Test extracts or residue with appropriate
bioassay.
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B. Bioassay guided chemical fractionation.
1. Chemical Extraction of Plant material.2. Test extracts or residue with appropriate
bioassay.3. Continue fractionation of positive materials.
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B. Bioassay guided chemical fractionation.
1. Chemical Extraction of Plant material.2. Test extracts or residue with appropriate
bioassay.3. Continue fractionation of positive materials.4. Assay new fractions.
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B. Bioassay guided chemical fractionation.
1. Chemical Extraction of Plant material.2. Test extracts or residue with appropriate
bioassay.3. Continue fractionation of positive materials.4. Assay new fractions.5. Identification of chemical components in active
fractions.
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B. Bioassay guided chemical fractionation.
1. Chemical Extraction of Plant material.2. Test extracts or residue with appropriate
bioassay.3. Continue fractionation of positive materials.4. Assay new fractions.5. Identification of chemical components in active
fractions.6. Assay of individual components.
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B. Bioassay guided chemical fractionation.
1. Chemical Extraction of Plant material.2. Test extracts or residue with appropriate
bioassay.3. Continue fractionation of positive materials.4. Assay new fractions.5. Identification of chemical components in active
fractions.6. Assay of individual components.7. Confirmation of toxic compound in large
animal.
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B. Bioassay guided chemical fractionation.
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Analysis of Plant Toxins
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Analysis of Plant Toxins
A. Plant Toxicity
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Analysis of Plant Toxins
A. Plant Toxicity1. Concentration of toxin in the plant can be
measured.
% dry weight, mg/g, ppm, ppb
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Analysis of Plant Toxins
A. Plant Toxicity and Toxic Dose.
1. Concentration of toxin in the plant can be measured.
2. Dose = Amount of plant material consumed
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Analysis of Plant Toxins
A. Plant Toxicity and Toxic Dose.
1. Concentration of toxin in the plant can be measured.
2. Dose = Amount of plant material consumed
3. Toxic Dose = mg/kg of toxin required to induce the clinical signs of toxicity
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Analysis of Plant Toxins
A. Plant Toxicity and Toxic Dose.
1. Concentration of toxin in the plant can be measured.
2. Dose = Amount of plant material consumed
3. Toxic Dose = mg/kg of toxin required to induce the clinical signs of toxicity.
4. Plant poisonings occur when 1 +2 > 3
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B. Metabolic Fate of Toxins in Animal
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Rumen
oxidation
reductase
ICA, Acetyl-ICA, Succinyl-ICA
Liver Detected
Metabolites
CH2OH
CO2H
CH2OH
CO2H
CO2H
O
OH
CO2H
O
OH
CO2H
O
OH
Blood Serum
Circulatorysystem
esterase
1
4
CO2H
O
OH2CO2H
O
OH 3
2
3(soluble enzyme
oxidation)
5
Figure 9. Summary of proposed metabolism of isocupressic acid after ingestion by cattle. ICA (1) isocupressic acid; (2) agathic acid;(3) dihydroagathic acid; (4) imbricatoloic acid; (5) tetrahydroagathic acid. Bold arrows indicate proposed major pathway. Dashed arrows indicate pathway as proposed by Lin et al. (1998) with ICA oxidized to 2 and then reduced to 3.
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B. Metabolic Fate of Toxins in Animal
1. Target compounds for diagnostics.
*
**
*
(13R)
(13S)
(13R)
(13S)RIC: m/z 397tetrahydroagathic acid
RIC: m/z 395dihydroagathic acid
TIC
2 4 6 8 10 12 14 16 18
Time (minutes)
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B. Metabolic Fate of Toxins in Animal
1. Target compounds for diagnostics.
2. Mechanism of toxicity.
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C. Diagnosis of Poisonous Plant Intoxications.
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D. Plant Taxonomy = Chemotaxonomy.
300 350 400 450 500 550 600 650 700 750 800
m/z
683
508
536468 578494404 627552346 726
508
683446 552 578482404346 538 711669
508
482468454 578552536 683372 627
683
697
578564494 669 739465 593360 436395
D. barbeyi(groups A&B)
D. occidentale(group C)
barbeyi x occidentale(group E)
D. glaucum(group D)
414
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D. Plant Taxonomy = Chemotaxonomy.
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The Whys and Wherefores of Quadrupole Ion Trap Mass Spectrometry
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The Whys and Wherefores of Quadrupole Ion Trap Mass Spectrometry
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qz = 4eV/mr2w2