Download - Points in Practical Virology
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Important points in practical virology
Abdou Mohammad Nagy, DVM, PhDDepartment of Virology
Faculty of Veterinary MedicineZagazig University, Zagazig 44519, Egypt
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Biological safety cabinetsClass I biological safety cabinet
http://www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/ch9-eng.php
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Class II biological safety cabinet
http://www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/ch9-eng.php
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Class III biological safety cabinet
http://www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/ch9-eng.php
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Work under BSC
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Tissue homogenizer Ice box Ice pack
Pestle & mortar
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What is Positive pressure protective suit (PPPS)?
Fresh, filtered air is typically supplied to the interior of a PPPS via overhead tubing. In addition to the physical barrier provided, the positive pressurization offers additional protection in the event of exposure through a defect or puncture in the suit, for if the suit’s integrity is compromised, air will be forced out instead of being sucked in. Extensive training with the PPPS is required to safely pursue research in a PPPS within a biosafety facility.
https://en.wikipedia.org/wiki/Positive_pressure_personnel_suit
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Do you want to work with Ebola virus?
http://www.naturalnews.com/047226_Ebola_outbreak_Dallas_health_care_worker.html
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Could you Identify improper biosafety problems?
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Common safety practices
https://www.google.com.eg/search?q=biosafety+symbols
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How do you use fire extinguisher?
http://www.fireonline.com.au/extinguishers
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Types of fire extinguisher
http://www.fireandsecurity-alarms.co.uk/fire-extinguishers/
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Liquid nitrogen hazards in virology lab
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University of Windsor students, overseen by Andrzej Sobiesiak, the head of the mechanical engineering department, right, transport a tank designed to carry 200 litres of liquid nitrogen along Wyandotte Street http://windsorstar.com/news/local-news/probe-launched-after-students-roll-hazardous-material-down-street
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Evaporated Liquid Nitrogen-Induced Asphyxia: A Case Reporthttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526498/
Petechiae (arrowheads) were revealed in periorbital areas of the decedent.
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What does your lab need to detect high levels of liquid N2??
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Virus inoculation in fertile eggs
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Good vs bad eggs
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Virus Inoculation in fertile eggs
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A) Curled and dwarfed embryos post IBV infection. B) Normal embryohttps://www.researchgate.net/publication/285581786_Phylogentic_analysis_of_recent_infectious_bronchitis_virus_isolates_from_broiler_chicken_farms_in_Kafrelsheikh_Egypt/figures?lo=1
Pock lesions https://en.wikipedia.org/wiki/Chorioallantoic_membrane#/media/File:Smallpox_CAM.png
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Mouse inoculation (I like it)Handling of mice
http://www.usp.br/bioterio/Artigos/Procedimentos%20experimentais/Routeadministration-4.pdf
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S/C inoculation
http://www.usp.br/bioterio/Artigos/Procedimentos%20experimentais/Routeadministration-4.pdf
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Intracerebral inoculation
http://www.usp.br/bioterio/Artigos/Procedimentos%20experimentais/Routeadministration-4.pdf
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Intraperitoneal inoculation Intranasal inoculation
http://www.usp.br/bioterio/Artigos/Procedimentos%20experimentais/Routeadministration-4.pdf
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Intravenous inoculation
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Cell culture equipment Inverted microscope
Roux bottleLeighton culture tube
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Primary cell culture preparation
http://www.cloud-clone.com/topic/Primary-cell-culture.html
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Cell subculture, freezing & Thawing
https://www.abmgood.com/marketing/knowledge_base/cell_culture_introduction.php
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CPE produced by viruses
http://abbs.oxfordjournals.org/content/41/12/998/F2.expansion
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http://www.virology.ws/2009/05/28/influenza-microneutralization-assay/
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What is the difference between two images?
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Different pH of cell culture medium
Pink YellowRed
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Thank you