Oxydo-reducteur systems and the thiol-dependent
antioxidant equipment in Melampsora
Benjamin Selles, Nicolas Rouhier, Eric Gelhaye
Oxygen is essential, but highly toxic
Formation of reactive oxygen species (ROS) due to electron transport chains and oxidase functioning
Release of ROS by plants as defense mechanism against pathogens
Diffusion within cells : importance to have an antioxidant equipment in all sub-cellular compartments
Oxydo-reducteur systems and the thiol-dependent antioxidant equipment in
Melampsora
ROS formation
Superoxide and peroxide scavenging
• Catalases : haem peroxidase (peroxisomes)
• Thiol peroxidases : haem free thiol dependent peroxidasesperoxiredoxins & glutathione peroxidasessome specific glutathione-S-transferases
• Methionine sulfoxide reductases : indirect massive detoxification through MetSO reduction
ROOH + ROH + H2ORSH2 RS2+
MetSO + Met + H2ORSH2 RS2+
H2O2
2H2O + O2H2O2 + H2O2
• Superoxide dismutases 02.- H2O2 + 0202
.-+2 H+ + 2 e-
SOD, catalases, thiol-peroxidases, methionine sulfoxide reductases
SOD, catalases, thiol-peroxidases, methionine sulfoxide reductases
Thiol peroxidase phylogenetic tree
MlpPrxQ
PrxQ
2-cysPrx
1-cysPrxMlp1-cys
PrxII
MlpPrxII
Gpx MlpGpx
Methionine sulfoxide reductases phylogenetic tree
MlpMsrBMlpMsrA
Glutathione-S-transferases
►Complex and widespread superfamilyComplex and widespread superfamily►Share thioredoxin foldShare thioredoxin fold►Prominent roles : Prominent roles :
phase II detoxication enzymesphase II detoxication enzymes Glutathione peroxidaseGlutathione peroxidase Nitrogen metabolism…Nitrogen metabolism…
►Poorly characterized in fungiPoorly characterized in fungi
Organism Physiology GST coding sequences
Ascomycetes
Candida albicans Human pathogen 10
Saccharomyces cerevisiae Baker yeast 11
Yarrowia lipolytica Hydrocarbons using yeast 12
Neurospora crassa Red bread mold 12
Magnaporthe grisea Plant pathogen 13
Aspergillus nidulans Human pathogen 17
Botrytis cinerea Plant pathogen 21
Sclerotinia sclerotiorum Plant pathogen 23
Aspergillus fumigatus Saprophyte and pathogen 23
Trichoderma reesei Cellulolytic 37
Basidiomycetes Melampsora laricis-populina Plant pathogen 9
Cryptococcus neoformans Plant and animal pathogen 9
Ustilago maydis Plant pathogen 11
Laccaria bicolor Ectomycorrhizal 21
Phanerochaete chrysosporium White rot fungus 28
Coprinus cinereus Saprophytic fungus 28
Postia placenta Brown rot fungus 36
Zygomycetes
Rhizopus oryzae Human pathogen 13
Phycomyces blakesleeanus Light response fungus 16
A. nidulans (AN10695)L. bicolor (LACBI1231002)
C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549)
C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218)
P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880)
P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790)
A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683)
A. nidulans (AN4905) N. crassa (NCU04109)
A. nidulans (AN9299) C. cinereus (CC1G00247.1)
C. cinereus (CC1G00666.1) L. bicolor (LACBI1188517)
P. chrysosporium (Phchr503) A. nidulans (AN3255) N. crassa (NCU05780)
P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271)
P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511)
S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1)
L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971)
L. bicolor (LACBI1240633) L. bicolor (LACBI1314362)
S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595)
A. nidulans (AN6563) N. crassa (NCU03826)
S. cerevisiae (YKL081W) S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C)
A. nidulans (AN9304) N. crassa (NCU10521.3) A. nidulans (AN0629)
N. crassa (NCU05706) C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C)
A. nidulans (AN10273) N. crassa (NCU04368) C. cinereus (CC1G04116.1)
C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379)
A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) C. cinereus (CC1G12612.1)
C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118)
P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1)
C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1)
C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1)
C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115)
P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) S. cerevisiae (YAL025C)
C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531)
N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850)
P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857)
0.1
Cluster3/Omega-like
Ure2p
Cluster
2
GTT2
EF1B
Cluster1/GTT1
Omega
Etherases
MAK16
Figure 1
4
1
1
2
1
A. nidulans (AN10695)L. bicolor (LACBI1231002)
C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549)
C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218)
P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880)
P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790)
A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683)
A. nidulans (AN4905) N. crassa (NCU04109)
A. nidulans (AN9299) C. cinereus (CC1G00247.1)
C. cinereus (CC1G00666.1) L. bicolor (LACBI1188517)
P. chrysosporium (Phchr503) A. nidulans (AN3255) N. crassa (NCU05780)
P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271)
P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511)
S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1)
L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971)
L. bicolor (LACBI1240633) L. bicolor (LACBI1314362)
S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595)
A. nidulans (AN6563) N. crassa (NCU03826)
S. cerevisiae (YKL081W) S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C)
A. nidulans (AN9304) N. crassa (NCU10521.3) A. nidulans (AN0629)
N. crassa (NCU05706) C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C)
A. nidulans (AN10273) N. crassa (NCU04368) C. cinereus (CC1G04116.1)
C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379)
A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) C. cinereus (CC1G12612.1)
C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118)
P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1)
C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1)
C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1)
C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115)
P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) S. cerevisiae (YAL025C)
C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531)
N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850)
P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857)
0.1
Cluster3/Omega-like
Ure2p
Cluster
2
GTT2
EF1B
Cluster1/GTT1
Omega
Etherases
MAK16
Figure 1
4 : oxidative stress, Glutathione peroxidase, nitrogen metabolism regulation
1
1
2
1
A. nidulans (AN10695)L. bicolor (LACBI1231002)
C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549)
C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218)
P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880)
P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790)
A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683)
A. nidulans (AN4905) N. crassa (NCU04109)
A. nidulans (AN9299) C. cinereus (CC1G00247.1)
C. cinereus (CC1G00666.1) L. bicolor (LACBI1188517)
P. chrysosporium (Phchr503) A. nidulans (AN3255) N. crassa (NCU05780)
P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271)
P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511)
S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1)
L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971)
L. bicolor (LACBI1240633) L. bicolor (LACBI1314362)
S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595)
A. nidulans (AN6563) N. crassa (NCU03826)
S. cerevisiae (YKL081W) S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C)
A. nidulans (AN9304) N. crassa (NCU10521.3) A. nidulans (AN0629)
N. crassa (NCU05706) C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C)
A. nidulans (AN10273) N. crassa (NCU04368) C. cinereus (CC1G04116.1)
C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379)
A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) C. cinereus (CC1G12612.1)
C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118)
P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1)
C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1)
C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1)
C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115)
P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) S. cerevisiae (YAL025C)
C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531)
N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850)
P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857)
0.1
Cluster3/Omega-like
Ure2p
Cluster
2
GTT2
EF1B
Cluster1/GTT1
Omega
Etherases
MAK16
Figure 1
4
1
1: Elongation factor
2
1
A. nidulans (AN10695)L. bicolor (LACBI1231002)
C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549)
C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218)
P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880)
P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790)
A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683)
A. nidulans (AN4905) N. crassa (NCU04109)
A. nidulans (AN9299) C. cinereus (CC1G00247.1)
C. cinereus (CC1G00666.1) L. bicolor (LACBI1188517)
P. chrysosporium (Phchr503) A. nidulans (AN3255) N. crassa (NCU05780)
P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271)
P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511)
S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1)
L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971)
L. bicolor (LACBI1240633) L. bicolor (LACBI1314362)
S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595)
A. nidulans (AN6563) N. crassa (NCU03826)
S. cerevisiae (YKL081W) S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C)
A. nidulans (AN9304) N. crassa (NCU10521.3) A. nidulans (AN0629)
N. crassa (NCU05706) C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C)
A. nidulans (AN10273) N. crassa (NCU04368) C. cinereus (CC1G04116.1)
C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379)
A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) C. cinereus (CC1G12612.1)
C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118)
P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1)
C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1)
C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1)
C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115)
P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) S. cerevisiae (YAL025C)
C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531)
N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850)
P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857)
0.1
Cluster3/Omega-like
Ure2p
Cluster
2
GTT2
EF1B
Cluster1/GTT1
Omega
Etherases
MAK16
Figure 1
4
1
1
2
1: Glutathione peroxidase
A. nidulans (AN10695)L. bicolor (LACBI1231002)
C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549)
C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218)
P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880)
P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790)
A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683)
A. nidulans (AN4905) N. crassa (NCU04109)
A. nidulans (AN9299) C. cinereus (CC1G00247.1)
C. cinereus (CC1G00666.1) L. bicolor (LACBI1188517)
P. chrysosporium (Phchr503) A. nidulans (AN3255) N. crassa (NCU05780)
P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271)
P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511)
S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1)
L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971)
L. bicolor (LACBI1240633) L. bicolor (LACBI1314362)
S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595)
A. nidulans (AN6563) N. crassa (NCU03826)
S. cerevisiae (YKL081W) S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C)
A. nidulans (AN9304) N. crassa (NCU10521.3) A. nidulans (AN0629)
N. crassa (NCU05706) C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C)
A. nidulans (AN10273) N. crassa (NCU04368) C. cinereus (CC1G04116.1)
C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379)
A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) C. cinereus (CC1G12612.1)
C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118)
P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1)
C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1)
C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1)
C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115)
P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) S. cerevisiae (YAL025C)
C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531)
N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850)
P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857)
0.1
Cluster3/Omega-like
Ure2p
Cluster
2
GTT2
EF1B
Cluster1/GTT1
Omega
Etherases
MAK16
Figure 1
4
1: Dihydroascorbate reductase, thiol transferase
1
2
1
A. nidulans (AN10695)L. bicolor (LACBI1231002)
C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549)
C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218)
P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880)
P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790)
A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683)
A. nidulans (AN4905) N. crassa (NCU04109)
A. nidulans (AN9299) C. cinereus (CC1G00247.1)
C. cinereus (CC1G00666.1) L. bicolor (LACBI1188517)
P. chrysosporium (Phchr503) A. nidulans (AN3255) N. crassa (NCU05780)
P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271)
P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511)
S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1)
L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971)
L. bicolor (LACBI1240633) L. bicolor (LACBI1314362)
S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595)
A. nidulans (AN6563) N. crassa (NCU03826)
S. cerevisiae (YKL081W) S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C)
A. nidulans (AN9304) N. crassa (NCU10521.3) A. nidulans (AN0629)
N. crassa (NCU05706) C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C)
A. nidulans (AN10273) N. crassa (NCU04368) C. cinereus (CC1G04116.1)
C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379)
A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) C. cinereus (CC1G12612.1)
C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118)
P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1)
C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1)
C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1)
C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115)
P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) S. cerevisiae (YAL025C)
C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531)
N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850)
P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857)
0.1
Cluster3/Omega-like
Ure2p
Cluster
2
GTT2
EF1B
Cluster1/GTT1
Omega
Etherases
MAK16
Figure 1
4
1
1
2 ?
1
Oxidation
ReductionSH
SH
SH
Disulfide bond formation, reduction, isomerisation
GRX
TRX, GRX
SOXPDI
Regulation of protein folding, activity
1. Spontaneous (with oxygen)2. Catalyzed by SOX3. Concomitant to a reducing activity
S
S
SH
SH
S
S
SSG
SH
SH
CxxC/S redox centers
PRX
GPX
ROS
Grx
GR
GSH NTR
Trx
NADPH
Msr
Trx
S-SGROS
Disulfide bond reduction by glutaredoxins & thioredoxins
MlpTrx4
MlpTrx1, 2, 3
MlpGrx1.1, 1.2
MlpGrx 2.2
MlpGrx 5
MlpGrx 2.1
MlpGrx 4
Trx/Grx domainsCGFS
Fe/S cluster formation
CGFS
DithiolCPYC
CPYS
TRX TRX
Erv1, TRX and CytC interaction in IMS; H2O production
Oxidation and isomerization by QSOX (Erv2 for fungi) and
PDI on unfolded proteins
Erv1 and TRX interaction in IMS; H2O2 production
Disulfide bond formation by sulfhydryl oxidasesand isomerisation by protein disulfide isomerases
SOX (ScErv1 like) and Erv2
First discovered in S cerevisiae and was essential for respiration and vegetative growth,
Intracellular localisation is:
IMS (SOX /scErv1 like)
ER (Erv2, Fungi)
Mitochondrial biogenesisProtein folding
Cytosolic Fe-S protein maturation
QSOXQSOX1 was initially identified from quiescent human
fibroblasts and his Intracellular localisation is:
ER, Golgi,
secretory granules and are located at the cell surface
Folding and regulation of secreted and parietal proteins
PDI and PDI like
Firstisolated from liver in 1963 Intracellular localisation is:
ER
Folding of proteins by isomerization of disulfide
bonds
Protein folding
CXXC CXXCN C
Domaine TRX 1 Domaine TRX 2
CXXC CXXCN C
Domaine TRX 1 Domaine TRX 2
CXXC
N C
Domaine TRX
Classe 1
Classe 2
Classe 5
Comparative genomic with other sequenced fungi
Fungus
scErv2 like
QSOX (HsQuiecin like)
Plants
Mammalian
SOX (scErv1 like)
Mammalian
Plants
Basidiomycete
Ascomycete
|Mellp (e_gw1.18.104.1)
Mellp (e_gw1.25.122.1)
Ascomycete
Ascomycete
Ascomycete
BasidiomyceteBasidiomycete
Basidiomycete
BasidiomyceteAsc
PDI class 2
PDI class 1
PDI class 5
EuGene.00020304
estExt_fgenesh2_pg.C_450075
estExt_fgenesh2_pg.C_240082
EuGene.00190108
02.- H2O2SOD OH.
Fe2+, Cu 2+
Fenton reaction
H2O + 1/2O2
Cat
NO.
OONO-
H2OSensingby TF
Trx, TpxNO2
Tpx, Grx, GST
RSNORSH
Tpx
Trx?
Protein thiol oxidation
ROS/RNS sensing and degradation
R(SH)2 RSSR, RSSR’, R(S)2RSSG
RSOHGSH
Grx, Srx? Trx, Grx
Grx? Trx, Grx
RSO2H RSO3H
Srx
PL-OOH
Tpx
PL
Conclusions
sulfenic
sulfinic sulfonic
02.- H2O2SOD OH.
Fe2+, Cu 2+
Fenton reaction
H2O + 1/2O2
Cat
NO.
OONO-
H2OSensingby TF
Trx, TpxNO2
Tpx, Grx, GST
RSNORSH
Tpx
Trx?
Protein thiol oxidation
ROS/RNS sensing and degradation
R(SH)2 RSSR, RSSR’, R(S)2RSSG
RSOHGSH
Grx, Srx? Trx, Grx
Grx? Trx, Grx
RSO2H RSO3H
Srx
PL-OOH
Tpx
PL
Conclusions
sulfenic
sulfinic sulfonic
Heme- thiolate Haloperoxidases
Hofrichter and Ulrich, 2006
2CPO 4 GSGIG.[1].PYDNNTLPYVAPG.[2].DSRAPCPALNALANHGYIPHDGRAIS.[4].QNAFLNHMGIANSVIELALT 74 gi 2498968 21 GSAAA ELDFEQWHPAGLG DLRCGCPAMNSLANHGFINHNGSNIT VNEVIPLMQEVFHLSEELAT 84 2CPO 75 .[1].AFVVCEYVTGSDCGDSLVNLTLLAEPHAFEHDHSFSRKD YKQGVANSND FIDNRN FDAET 135 gi 2498968 85 IVTGLAVLSADDPASGIFNLDMLNRHNIFEHDASLTRKD.[1].YLGGDGHTID.[2].TLDEFL.[2].FDGKE 149 2CPO 136 FQTSLDVVAGKTH FDYADMN EIRLQRESLSNELDFPG WF TESKPIQN VESGFIFA 190 gi 2498968 150 WIDLNDAAAARYA.[2].LDSREKN.[1].SFLYQDQQLITSYGETI.[1].YF.[1].TMVDPRSN.[1].TSAEFVRI 210 2CPO 191 LVSDFNLP.[1].NDENPLVRIDW WKYW.[1].TNESF.[4].GWH.[1].PSPAREIEFVTSASSAVLAASVTS 252 gi 2498968 211 LFTEERLP VRKGGSAREKR.[2].GSRW PAMSF SWR CAPQRSSLACRSTSVRLQSRPLTR 267 2CPO 253 TP SSLPSG.[2].GPGAEAVPLSFASTMTPFLLAT.[1].APY YAQDPTLGP 297 gi 2498968 268 CH.[1].SGLPSG LPRTTRVSVRGISLSLSGGLRR RPF.[1].FVLDRHLSF 311
Pfam 1328: Peroxidase_2
Peroxidase, family 2. The peroxidases in this family do not have similarity to other peroxidases.
BLAST withESTEXT_FGENESH1_PG.C_240160
Ajellomyces capsulatus NAm1 0Ashbya gossypii ATCC 10895 0Aspergillus clavatus NRRL 1 0Aspergillus nidulans 4(5)Aspergillus flavus 0Aspergillus fumigatus 1(2)Aspergillus niger 2Aspergillus oryzae 2Botryotinia fuckeliana B05.10 7Candida albicans 0Candida tropicalis 0Chaetomium globosum 3Clavispora lusitaniae ATCC 42720 0Coccidioides immitis RS 0Cryptococcus neoformans var. neoformans JEC21 0Coprinopsis cinerea okayama7#130 5Debaryomyces hansenii CBS767 0Fusarium oxysporum 0Gibberella moniliformis 0Laccaria bicolor 1(2)Magnaporthe grisea 4(5)Malassezia globosa CBS 7966 0Melampsora 16Mycosphaerella graminicola 15(16)Nectria haematococca mpVI 0(3)Neosartorya fischeri NRRL 181 0Neurospora crassa OR74A 1Phaeosphaeria nodorum SN15 10(11)Phanerochaete chrysosporium RP-78 1(4)Phycomyces blakesleeanus 0Pichia stipitis CBS 6054 0Postia placenta 0Puccinia graminis f. sp. tritici CRL 75-36-700-3 0Rhizopus oryzae RA 99-880 0Saccharomyces cerevisiae 0Schizosaccharomyces pombe 972h- 0Sclerotinia sclerotiorum 1980 4Sporobolomyces roseus IAM 13481 0Trichoderma reesei QM6a 1Uncinocarpus reesii 1704 0Ustilago maydis 521 0Yarrowia lipolytica CLIB122 0
0.1
2407|AciVP
3842|CdCII01
2403|CcinCII01
2404|ArCII
2307|BaVP01
2450|BspVP
2395|BspVP
2422|TvCII01
2294|PrCII03
2451|BspVP
3894|TvCII02 IFO30340
2419|TvCII02 KN9522
3857|TvCII02 PRL572
2424|TvCII05
2423|TvCII02
2425|TvCII03 KN9522
2418|BaLiP
3852|CsuLiP01
3830|CsuLiP02
2412|PcLiPA
3854|PcLiPA SC26
3882|GfCII01
2405|GapCII01
3874|GauCII01
3847|TvCII03
2408|TvCII03 CU1
2332|PcCII01
2449|MagCII03
3887|GzCII01
2447|MagCII01
2448|MagCII02
mpfgenesh2 pg.69 55[Mellp1 112
mpfgenesh2 pg.69 50[Mellp1 112
mpEuGene.00690043[Mellp1 94316
mpestExt fgenesh1 pg.C 240160[
mpfgenesh2 pg.79 26[Mellp1 113
mpfgenesh2 pg.24 191[Mellp1 10
mpfgenesh1 kg.C scaffold 15000
mpestExt fgenesh1 kg.C 680007[
mpestExt fgenesh1 kg.C 680005[
mpfgenesh2 pg.11 10[Mellp1 104
mpestExt fgenesh1 pm.C 330027[
mpestExt fgenesh1 kg.C 420016[
MnP3pc.27.12.1[whiterot1 70578
MnP1pc.15.23.1[whiterot1 66512
MnP4pc.15.18.1[whiterot1 66507
MnP2pc.64.57.1[whiterot1 76161
MnP5pc.9.126.1[whiterot1 78685
Mlp Prx 12 isoforms
Peroxidase class II(Peroxibase)
All predicted secreted
(target P)
Function ??