Download - Neuromics Presentation V4
Tools and Capabilities Focus on cell based assays
and gene expression analysis
for early phase Drug Discovery
3/5/2011-Version 4
3/5/2011
Focus:
Provide researchers a subset of tools and methods to enable
successful outcomes in early phase drug discovery. Failure is
success when determined early.
Value (simplified):
Physiologically relevant primary human (STEMEZ™), rat and
mouse cells Better in vitro screening of targets and molecules
Reduced animal model use
Shorten development timeline
Lower downstream costs
Images: STEMEZ™ hNP1™ can be
differentiated into various neuronal
subtypes under defined conditions.
DAPIGFAP Antibody
• > 80% Tuj1+
• > 60% MAP2+
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Why Neuromics? Standing out in a crowded room
We have a experience serving many large Pharmas, Biotechs,
Academic and Government Labs.
Customer success focused
Expert technical support
Detailed protocols and methods
Quick replacements and reorders
Large expert resource network
Published expert input
Customer feedback and data sharing through
social media outlets and blogs
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Testimonial: got 10 million cells total after extraction from the tissue. At Day 4 they all developed long axons.
Thank you so much for the replacement." Dr. Lidia Gardner, University of Tennessee HSC..
Gene Expression
Analysis
Media/3-D
Gels/ECMS
Apoptosis
Detection
Cell Migration
Assays
Growth
Factors/Markers Applications include:
•Proliferation and
neurogenesis
•Neuronal differentiation
•Neurite outgrowth
•Cell migration
•Neurotoxicity screening
•Cell based compound
screening
•G-protein coupled receptors
•Ion channels
•Stem cell transplantation
Derived and
manufactured
neural cells from
human
embryonic stem
cell line H9
(WA09).
Stable, Potent and Cost Effective Cells
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Markers/Antibodies Growth Factors/Culture Media Gene Expression Analysis Apoptosis Detection
Imges: GFAP (Catalog#:
aCH22102) Staining of of
Astrocytes in the Ventral
horn. Courtesy of Dr. Qi-lin
L. Cao, University of
Louisville School of
Medicine. Rat spinal cord
homogenate WBs: NF-H,
NF-M,, NF-L,, NF66 and
GFAP.
STEMEZTMhNP1
Human Neural
Progenitors treated
with hGDNF
cultured in
STEMEZ hNP1
Neural Progenitor
Expansion Media
i-Fect knockdown of Delta Opioid
Receptor. Molecular Pain 2005,
1:29
Jurkat cells dually
stained with Hoechst
and Polycaspase
Assay Kit,
Assay kits, Qualified Media and Reagents-Sample
Images
Cell Based Assays-Examples
STEMEZ hN2 Human Neural Discovery Kit
Phenotyping
Electrophysical and FLIPR Data Provided upon request
Neural phenotypes derived from N2 cell
lines. (A) Phase contrast image of
differentiated culture. (B) Network
including post-mitotic motoneurons
(HB9). (C) Cholinergic neuron. (D) Tuj-1
positive cells that are DAT-positive
(dopamine transporter; closed arrow)
and DAT-negative (open arrow). (E)
Gabaergic neurons, inset illustrates
GABA in axon, but not the dendrites
(arrow).
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Tools and Capabilities
Cell Based Assays-Examples
STEMEZ™ hNP1™ Human Neural Progenitor Kit is an efficient tool to quantitatively
detect both stimulators and inhibitors of cell migration when used with the OrisTM Cell
Migration Assay Kit (Platypus Technologies). Method development to date indicates that
the assay has the potential for adaptation as a homogenous HTS-suitable cell-based
assay.
Cell Based Assays-Examples
Primary Rat Neurons and Astrocytes
Transfection of functional HeV
glycoproteins and infection with HeV
pseudotyped virions.In order to establish
the feasibility of carrying out the proposed
experiments in primary neurons, we show
(figure ) that our assays are amenable to
use in primary neurons. In the experiment, Combined Hippocampus, Cortex, and Ventricular -E18
(Neuromics) were plated, and at 3 days were transfected with plasmids encoding HeV G/F as well
as YFP. On the following day, these cells were infected with HeV or VSV pseudotyped viruses
bearing RFP. In the figure, (A) the phase contrast photos show the differentiated neurons; (B)
upon excitation for RFP, the red fluorescence indicates neurons infected by HeV pseudotyped
virions; (C) upon excitation for YFP, and the green fluorescence shows the efficiency of
transfection in neurons. This experiment indicates that the proposed experiments can be carried
out in primary neurons, which are transfectable and infectable in our systems, and thus supports
all the proposed aims. Data Courtesy of Dr. Matteo Porotto, Weill Cornell Medical College. Larger
Image
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Cell Based Assays-Examples
Primary Rat Neurons and Astrocytes
Data Courtesy of Evanna Gleason, Louisiana State University
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Gene Expression Analysis It’s all about delivery!
i-FectTM siRNA delivery kit : Success in delivering siRNA to
DOR, hTERT, The β3 subunit of the Na+,K+-ATPase,
rSNSR1, NTS1. NAV1.8, Survivin, TRL4, Flaviviruses and
more.
Tools for delivering plasmids, proteins, antisense and
other small RNA and DNA molecule.
Transfection Delivery: Customer Publications
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More Key Product Category Links:
Antibodies-Markers
Apoptosis Research Reagents -includes:
detection kits, antibodies and proteins
Proteins Recombinant and natural proteins-
includes a wide selection of cell growth factors.
Transfection Reagents-Gene Expression Analysis
Cell Growth Media
Clear Understanding of where Neuromics could have
a positive impact. More intense in vitro screening of compounds and
targets.
Better candidates for downstream processes.
Propose solutions based on this understanding
Follow up, tweak and improve
3/5/2011
Pete Shuster
CEO and Owner
Direct Phone: 612-801-1007
Social Networking:
www.neuromics.com
www.neuromics.net
neuromics.blogspot.com
sirnatransfection.blogspot.com
3/5/2011