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Life Science Summer Institute 2006
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This project was funded through the San Diego Workforce This project was funded through the San Diego Workforce Partnership, Inc. by a grant awarded under the President's High Partnership, Inc. by a grant awarded under the President's High Growth Job Training Initiative, as implemented by the U.S. DeparGrowth Job Training Initiative, as implemented by the U.S. Department tment of Labor's Employment and Training Administration of Labor's Employment and Training Administration
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What we love about LSSI:Participating in effective & rewarding professional growth
Having resources to implement biotechnology in the classroom
Showing students how this science relates to the “real world”
Working with talented teachers from throughout the county
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What we love about LSSI:Learning about biotechnology techniques, industry needs, and cutting-edge science
Bringing “workable” biotech into the classroom
Discussing potential biotech and career paths for our students
Seeing the multi-disciplinary nature of biotechnology
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Who was involved:
• SCBC at Miramar College: Sandy Slivka & Sharon Price
• Amgen/Bruce Wallace curriculum: Marty Ikkanda
• San Diego Workforce Partnership• BioCom• Biogen Idec Community Lab• 20 teachers from San Diego County
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Week 1
• Safety• Intro to biotech industry• Research local biotech companies and
institutes• Identify California state standards
associated with biotechnology• Perform biotech labs• Bioethics discussion
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Lab Safety Lab Safety -- PPEPPE(Personal Protective Equipment)(Personal Protective Equipment)
• Closed-toe shoes
• Pants• Lab coats• Safety glasses• Appropriate
gloves
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Emergency Procedures
• KNOW the locations of your:
• Emergency exit and have back up ready if needed
• Staging Area• Intercom / Phone• First Aid kit and AED• Fire extinguisher• Eyewash/safety shower.
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Lab 2 Restriction Digest
pARA & pKAN-R
Lab 1 IntroRecombinant Pathway
Lab 3 Ligation
Lab 4a Confirmation of Digestion
Lab 2a
pARA-R
Non-recombinant Pathway
Lab 4 Confirmation of Restriction & Ligation
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Lab 5a Transformation
w. pARA-R
Lab 5 Transformation w.
Recombinant Plasmid
Lab 6 Culture of E. coli
Lab 7 mFPProtein
Purification
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Lab Technologies
• Micropipetting• Gel Electrophoresis• Restriction Enzyme Digests• Plasmid Ligation• Bacterial Transformation-heat shocking• Cell Culture• Protein Purification• DNA Extraction
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Weeks 2 & 3
Field trips to various biotech companies and research institutes.
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Types of Exposure• Demonstration of utilization of Basic Science Concepts
in Applied Fields • Exploration of Drug Development Process • Overview of Company Structure/Function • Organizational/Department Lead Panel • Laboratory/Facility Tour • Scientist Panel • Scientist Presentation • Hands-on Demonstration • Career Exploration/Options • Information on Networking Events • Investor Relations/Communications
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Genentec
San Diego Natural History Museum
Karl Strauss
Gen_Probe
The Burnham Institute
SALK
Pfizer
ISIS
Nanogen
Dow
Alexion / Accumetrics
CRES / Wild Animal Park
Invitrogen
Genoptix
Miramar College - SCBC
Biogen Idec
Summer 2006
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Isis Pharmaceuticals
• Founded in 1989• Carlsbad, CA• Holds 1500+ patents• 250 employees• Research & development, small scale
manufacture of Vitravene drug
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Sectors
• Medical/pharmacology sector–Antisense drugs (“Vitravene”)
• Industrial/environmental sector–Biosensors (“Ibis T5000 TIGER”)–Small molecule division
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Antisense Drugs
• Target specific sequence of mRNA
• Block translation of mRNA into amino acid sequence
• Result = target protein can’t be made
http://www.biotech.ubc.ca/MolecularBiology/AntisenseRNA/antisense1.gif
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Alexion“engaged in discovering and developing a portfolio of antibody therapeutics targeting severe unmet medical needs. AAT discovery efforts are focused on cancer, autoimmune disorders and infectious disease.”
– Antibody therapeutics focused on cancer, autoimmune disorders, and infectious diseases
• Thrombocytopenia (depleting of platelets due to chemotherapy
– Genetically altered antibody to increase production of platelets
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Tools• Genetic Engineering
– Propriety assays– High throughput screening– Protein modification– Protein purification– Bacterial and mammalian expression of
antibodies and fragments
Tecan
“Tecanator”
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Core Technology• CoALT – customized human antibody
libraries– Generation of highly specific, fully human antibodies– Rapid generation of fully human or humanized
antibodies– Facilitates a seamless transition from genomic
research to drug development– Direct sources of antibodies for therapies
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Accumetrics“Mission” Statement
Accumetrics is a privately-held biotech company focused on the design, development, manufacturing, and marketing of innovative point-of-care cardiovascular diagnostic products
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Products - Overviewoptimize the effectiveness of
antiplatelet therapies guide treatment decisions to
reduce risk of bleeding complications
Tools to Monitor Platelet function:
Advantages:easy, automated, rapid & accurate
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Products - DetailsThe VerifyNow™ System consists of an instrument, disposable assays & controls.
• Instrument is an optical detection system which measures platelet induced aggregation (~$5000 per instrument)
• Assays based on platelet receptor sites (Aspirin, P2Y12, and IIb/IIIa so far)(single-use $20-$50 each)
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DOW San Diego
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Dow: Protein of Interest
• Dow Pharma, Dow Agra send proteins to be expressed
• Use Pseudomonus fluorescens• Microliter to 1500 liter fermenters• Most proteins processed code for
enzymes• Human growth/antibodies…current focus
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Dow: Amplify/Analyze
• Grow up in tubes• Analyze down to DNA
sequencing• Shakers to grow• Robotic device for
making cell preps• Billion cells per mL
• Protein expression• 2-d gels• Separate by size and
isoelectric points• pH gradient strips• Thin layer acrylimide• High voltage 8kV
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Dow: Process
• PCR amplify• Modify• Express• Analyze/What works• Scale up• Ferment• Recover
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Nanogen Corporate Headquarters10398 Pacific Center Court
San Diego, CA 92121
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Mission• Nanogen's mission is to
become a leading supplier of advanced diagnostic solutions to the medical community and to clinical researchers.
• Multiple markets include: – research– clinical – point-of-care
• Technology integrates advanced microelectronics & molecular biology on proprietary semi-conductor microchips.
• Diagnostic Research Biotechnology
• A production & RD mature company
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some recent news
• Nanogen Launches Cystic Fibrosis Analyte Specific Reagents 23 March 06specific reagents (ASRs) to detect 23 wild type and mutation sequences associated with cystic fibrosis (CF).
• Nanogen Announces Type II Diabetes Research Collaboration 15 May 06
• Acquires Italian Molecular Diagnostics Company Amplimedical 20 April 06
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Accomplishments• >350 Patents & >250 Licensing Agreements• 17 Companies founded to develop Institute technologies
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Research Directions• Chemistry and Proteomics• Stem Cell Biology• Cell Biology• Regulatory Biology• Metabolic Research• Computational and Theoretical Biology
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A non-profit, research institute with:Three research centers:• NCI Cancer Center• Del E. Webb Center for Neurosciences and Aging• Infectious and Inflammatory Disease CenterThree technology centers:• San Diego Center for Chemical Genomics• Stem Cell Research Center• Center on Proteolytic Pathways
80% of funding is due to federal grants.
www.burnham.org
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Tools and Technology
• Molecular Library Screening Network
• Stem-Cell “biologics-free” culture growth
• Gene array • DNA library
screenings• Molecular Biology
such as gene therapy
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More Tools and Technology
• Signal transduction for proteases
• Microscopy• Molecular targeting• 3-D molecular maps• Monoclonal
Antibodies such as anti-cancer drugs
• And more . . .
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Gen-Probe Incorporated10210 Genetic Center DriveSan Diego, CA 92121-4362
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Employees: 1000 + International Hqtrs on Genetic Center Drive****80 Hours in 9 Days (10 hour days)
Stage of Growth: 3rd stage: Production and R&D - 23 years old
Background/History: Founded in 1983Five Core Technologies:1) Ribosomal RNA (rRNA)2) Target Capture3) Transcription-Mediated Amplification4) Hybridization Protection Assay (HPA)5) Dual Kinetic Assay (DKA)
Financial Status: Stock Share Price: $53.46 – 7/12/06 1600Z
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.
Conclusion:
1. Diverse company with a niche in the diagnostic arena – both technical (equipment) and process (kits)
2. Established and thriving company
3. Good growth potential because of aggressive attitude toward applications (tests) and understanding of need (marketing strategy address social concern – HIV and Prostate Cancer)
4. Employment potential strong – variety of opportunities
a. legal, public affairs, technical in both lab and non-lab areas (I.T. and Biology)
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Lunch with scientists at Gen-Probe
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Karl Strauss:Industrial Biotechnology
Production of fermented beverages
LSSI Group at Karl Strauss
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THE Scientist at Karl Strauss
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Pfizerhttp://www.pfizer.com/pfizer/are/index.jsp
• Our Mission - We will become the world's most valued company to patients, customers, colleagues, investors, business partners, and the communities where we work and live.
• Our Purpose - We dedicate ourselves to humanity's quest for longer, healthier, happier lives through innovation in pharmaceutical, consumer, and animal health products.
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Research Highlights @ Pfizer La Jolla
• Cancer• Goal - To improve patient survival rates primarily in
the area of novel agents targeting anti-angiogenesis, signal transduction, and cell cycle regulation.
• Viral Disease• Goal - To identify new antiviral therapies to treat
these serious viral diseases
• Diabetes & Obesity• Goal - Treatment of diabetes and obesity
specifically by suppressing appetite, increasing energy expenditure, sensitizing diabetic patients to the actions of insulin and treating diabetic complications
• Disease of the Eye• Goal - Seeking to identify and develop medications
that maintain vision and prevent blindness.• Specifically focusing on anti-angiogenesis
compounds to treat age-related macular degeneration, diabetic retinopathy, and diabetic macular edema.
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Pfizer Core TechnologiesLarge corporate umbrellayields a broad spectrum oftechnologies:• Monoclonal antibodies• Molecular Biology
– Genetic Engineering– DNA Technology– Tracers
• Cell Culture
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Employment
• 100,000 full-time global employees
• Job Openings– 100 globally– 48 in Oceanside
• Ranging from Supervisors, Scientists, Engineers, Interns, Human Resources, and Technical Trainers
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Sector and Growth• Genentech works in the medical and
pharmaceutical sector.– Focuses on Oncology, Immunology, and
Tissue Growth and Repair• They are in several stages of growth for
their various products.– Development for some products.– Research for others.– Production and marketing for other
products.• The Oceanside site is a manufacturing site.
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Tools• Recombinant DNA
processes• Oceanside site has
large scale equipment for manufacturing– Large Bio-reactors,
etc.
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CRES
Sustainable Populations
Genotyping California Condors forpedigree analysis and genetic mapconstruction.
Development of non-invasive imaging techniques to detect pregnancy in Giant Pandas.
Maternal care strategies in GiantPandas and other bears.
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Founded in 1987 and incorporated in 1989 with headquarters in Carlsbad, CA
Conducts business in more than 70 countries
25,000 products and services to support disease research, drug discovery, and commercial bio-production
Stage of Growth: Production and R&D
Invitrogen Corporation1600 Faraday AvenueCarlsbad, California 92008Phone: (760) 603-7200Fax: (760) 602-6500www.invitrogen.com
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Applications Antibodies & ImmunodetectionBead–Based SeparationsBio-ProductionCell CultureCloning
DNA & RNA PurificationFlow CytometryGene Expression ApplicationsHLA DiagnosticsTransfection
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Areas of FocusAvian Influenza
Vaccine BioproductionBiodefense
Infectious diseases and pathogen detectionBioinformatics
Vector software for PC & MacDrug Discoveries and Solutions
Kinases & Phosphatases, nuclear receptors, Ion Channels, G-Protein Coupled Receptors
Proteomics and Protein Expression Separation, Quantitation/Mass Spectrometry, Protein Expression
Stem Cell Research Isolation, Characterization, Expansion, Differentiation
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Genoptix has developed a breadth of services that focus on the challenges of each phase of patient management to help in effective diagnosis, optimal treatment decisions and monitoring of hematopoietic diseases such as Leukemia, Lymphoma and a non-hemopoietic disease, Breast Cancer metastatic stage 4.
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Technologies• Morphology and
Histopathology– Microscopic analysis
of tissues • 6 color flow cytometry
– Examination of single suspended cells using laser beam which gives information about size and markers on cells
• Cytogenetics– Looking at
chromosomes using karyotypes
• FISH (Fluorescence in situ Hybridization)– Examination of cell
abnormalities not requiring cells in division
• RT-PCR (Reverse Transcriptase Polymerase Chain Reaction)
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COMPASS• Provides a clear, consistent summary
report that assimilates everything a physician needs to know for appropriate patient intervention in hematopoieticdisorders. A "one glance" summary report correlating all technologies for optimal diagnostic review, decision making and patient management
Diagnose TreatPredict Monitor
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Corporate VisionCorporate Vision
With passion, purpose and partnerships, we transform scientific discoveries into
advances in human healthcare.
With passion, purpose and partnerships, we transform scientific discoveries into
advances in human healthcare.
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Biotechnology Sector
• This company is involved with research, development, production, and sales of novel medical and pharmaceutical therapies in the fields of:– Oncology– Neurology– Immunology
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Core Technology
Monoclonal Antibodies make up the core of their research
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Thanks for the Hospitality!
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Curriculumdevelopment
Networking withcolleagues
LSSI Week 4
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Updating Sharon’scalendar
Teacher Presentationsof labs
LSSI Week 4
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Camille Fowler
“This was a great opportunity to meet dedicated teachers, meet industry
professionals, and bring exciting technology to my classroom”
LSSI Week 4Teacher Personal
Statements
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“My Chemistry students are really going to benefit from this experience.
I’m looking forward to integrating biotechnology ideas into our curriculum.”
Stephanie Gaudreau
LSSI Week 4Teacher Personal
Statements
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Steve Bartram
“Biotech Rules! This is the greatest group ever!”
LSSI Week 4Teacher Personal
Statements
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