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Ti liu chia s ti: wWw.SinhHoc.edu.vnS dng Pubmed
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Tm kim trnh t sinh hc 7
Tm kim trnh t sinh hc qua NCBI
Click
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Tm kim trnh t DNA
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Ti liu chia s ti: wWw.SinhHoc.edu.vnTm kim trnh t qua m s truy cp
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M s truy cp ca mt trnh t l m s do cc nh qun trCSDLSH t cho mt trnh t,thng c dng :8 k t: 2 chv 6 s vdnhAY6906406 k t: 1 chv 5 s vdnhU20068
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Ti liu chia s ti: wWw.SinhHoc.edu.vnTM KIM TRNH T SINH HC QUA M S
TRUY CP
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Ti liu chia s ti: wWw.SinhHoc.edu.vnKt qu tm trnh t DNA qua m s truy cp
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Tm kim trnh t qua tn gene
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Ti liu chia s ti: wWw.SinhHoc.edu.vnnh dng FASTA
FASTAl mt gii thut bt cp trnh tcDavid J. Lipman v William R. Pearsonmiu tln u tin vo nm 1985 (Rapid and sensitiveprotein similarity searches).
Nhiuphnmm tin sinh hccnd liu trnht gene hoc protein theo kiu nh dngFASTAnh v d minh hodiy:
>tn trnh tgattctcacttggtctgctgcaaggacgcggaccattaaaactgttcatggcccttgtg
gcgttctcgtttcctaacaatcccaccaacagcagggatactaaaaagatggggaac
gatcaaaaaatcaaaagctatcaatgtcttgagagggttcaggaaagagattggaagat ct aacatctt aaca a ac ca aca ca c t att ttat tt att15
http://vi.wikipedia.org/wiki/B%E1%BA%AFt_c%E1%BA%B7p_tr%C3%ACnh_t%E1%BB%B1http://vi.wikipedia.org/w/index.php?title=David_J._Lipman&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=William_R._Pearson&action=edit&redlink=1http://vi.wikipedia.org/wiki/1985http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2983426http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2983426http://vi.wikipedia.org/wiki/Ph%E1%BA%A7n_m%E1%BB%81mhttp://vi.wikipedia.org/wiki/Ph%E1%BA%A7n_m%E1%BB%81mhttp://vi.wikipedia.org/wiki/Ph%E1%BA%A7n_m%E1%BB%81mhttp://vi.wikipedia.org/w/index.php?title=Tr%C3%ACnh_t%E1%BB%B1&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=Tr%C3%ACnh_t%E1%BB%B1&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=Tr%C3%ACnh_t%E1%BB%B1&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=Tr%C3%ACnh_t%E1%BB%B1&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=Tr%C3%ACnh_t%E1%BB%B1&action=edit&redlink=1http://vi.wikipedia.org/wiki/Ph%E1%BA%A7n_m%E1%BB%81mhttp://vi.wikipedia.org/wiki/Ph%E1%BA%A7n_m%E1%BB%81mhttp://vi.wikipedia.org/wiki/Ph%E1%BA%A7n_m%E1%BB%81mhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2983426http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2983426http://vi.wikipedia.org/wiki/1985http://vi.wikipedia.org/w/index.php?title=William_R._Pearson&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=David_J._Lipman&action=edit&redlink=1http://vi.wikipedia.org/wiki/B%E1%BA%AFt_c%E1%BA%B7p_tr%C3%ACnh_t%E1%BB%B1 -
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Ti liu chia s ti: wWw.SinhHoc.edu.vnCch ly trnh t theo nh dng FASTA
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Mt s m s truy cp ca RefSeq database
1. mRNAs and Proteins NM_123456 Curated mRNA
NP_123456 Curated Protein
NR_123456 Curated non-coding RNA
XM_123456 Predicted mRNA XP_123456 Predicted Protein
XR_123456 Predicted non-coding RNA
2. ChromosomeNC_123455 Microbial replicons, organelle
genomes, human chromosomes
4. Assemblies
NT_123456 Contig 17
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Ti liu chia s ti: wWw.SinhHoc.edu.vnV d 1: NM_123456 Curated mRNA
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NM_123456
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Ti liu chia s ti: wWw.SinhHoc.edu.vnV d 2: NC_12345
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S
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Ti liu chia s ti: wWw.SinhHoc.edu.vnKt qu tm kim b gene
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Blast trnh tSo snh cc chui sinh hc khc nhau(chuiamino-acid, chui DNA), tm kim trnh t tng ng
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http://vi.wikipedia.org/w/index.php?title=C%E1%BA%A5u_tr%C3%BAc_ch%C3%ADnh&action=edit&redlink=1http://vi.wikipedia.org/wiki/Amino_acidhttp://vi.wikipedia.org/wiki/Amino_acidhttp://vi.wikipedia.org/w/index.php?title=Chu%E1%BB%97i_DNA&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=Chu%E1%BB%97i_DNA&action=edit&redlink=1http://vi.wikipedia.org/wiki/Amino_acidhttp://vi.wikipedia.org/wiki/Amino_acidhttp://vi.wikipedia.org/wiki/Amino_acidhttp://vi.wikipedia.org/w/index.php?title=C%E1%BA%A5u_tr%C3%BAc_ch%C3%ADnh&action=edit&redlink=1 -
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Ti liu chia s ti: wWw.SinhHoc.edu.vnBLAST thcs l mth cc chng trnh (bao gmcchng trnh blastall). Sau y lmtschng trnh trong h,cspxp theo tht quan trngca n:
Nucleotide-nucleotide BLAST (blastn): khi a vo mt DNA truy vn,strv ccchui DNA gngingnhttcsdliu DNA m ngi dng chnh.
Protein-protein BLAST (blastp): khi a vo mt protein truy vn,strv cc chuiprotein gngingnhttcsdliu protein m ngi dng chnh.
Position-Specific Iterative BLAST (PSI-BLAST): Mt trong nhng chng trnhBLAST mi nht, chng trnh ny dng tm kim cc mi quan h xa ca mtprotein. Trc tin, mt danh sch cc protein lin quan s c to ra. Sau ,nhng protein ny ckthp thnh mt "profile" didngchui trung bnh. Mtcu truy vn timtcsd liu protein sc thc thi nh profile ny, v mtnhm lnhn cc protein c tm thy. Nhm ln ny lic dng to ra mtprofile khc, v qu trnh ny c lp li.
Bng cch thm cc protein lin quan vo vic tm kim, PSI-BLAST tr nn tthntrong vic la ra cc mi quan h tin ha cch xa nhau hn l phn mmchunprotein-protein BLAST.
Nucleotide-protein 6-frame translation (blastx): Chng trnh ny so snh cc snphmchuyni(tru tng) sang 6-khung camtchui nucleotide truy vn(c 2di)vimtcsdliuchui protein. Qu trnh ny c thrtchm.
Nucleotide-nucleotide 6-frame translation (tblastx): Chng trnh ny l chmnht
trong h BLAST. N chuynchui nucleotide truy vn thnh mi 6-khung (frame) cth v so snh cc proteins to thnh. Mc tiu catblastx l tm kimmi quan hrtxa gia cc chui nucleotide.
Protein-nucleotide 6-frame translation (tblastn): Chng trnh ny chuyncsdliuch thnh mi 6-khung (frame) v so snh vichui protein truy vn .
Large numbers of query sequences (megablast): Khi so snh mtslngln ccchuiu vo qua chmt BLAST dng dng lnh, "megablast" l nhanh hnrtnhiuso vichy BLAST nhiuln.
Ti li hi t i W Si hH d
http://vi.wikipedia.org/wiki/Proteinhttp://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/w/index.php?title=M%E1%BB%91i_quan_h%E1%BB%87_ti%E1%BA%BFn_h%C3%B3a&action=edit&redlink=1http://vi.wikipedia.org/wiki/Protein -
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Ti liu chia s ti: wWw.SinhHoc.edu.vnSo snh hai trnh t bng blast
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Ti liu chia s ti: wWw.SinhHoc.edu.vnSo snh H5N1 v streptococus
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Load trnh t 1
Load trnh t 2
Nhn th
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Ti liu chia s ti: wWw.SinhHoc.edu.vnKt qu bng so snh hai trnh t
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Ti liu chia s ti: wWw.SinhHoc.edu.vnKt qu so snh H5N1 v Streptococus
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Ti liu chia s ti: wWw.SinhHoc.edu.vnPhn tch trnh t DNA: tm khung c m (ORF)
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Thit k primer
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Ti liu chia s ti: wWw SinhHoc edu vnKim tra c hiu mi bng blast
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Ti liu chia s ti: wWw.SinhHoc.edu.vnKim tra c hiu mi bng blast
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Ti liu chia s ti: wWw SinhHoc edu vnN l tid bl t
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Ti liu chia s ti: wWw.SinhHoc.edu.vnNucleotide blast
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Ti liu chia s ti: wWw SinhHoc edu vnNh t h t i
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Ti liu chia s ti: wWw.SinhHoc.edu.vnNhp trnh t mi
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agtggcttgccgctacgactccactgtctgaaaacagccgac
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Ti liu chia s ti: wWw.SinhHoc.edu.vnPrimer blast
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Tnh tng ng ca ca cc primer
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c hiu ca mi
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Ti liu chia s ti: wWw.SinhHoc.edu.vn c hiu ca mi
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Ti liu chia s ti: wWw.SinhHoc.edu.vn c hiu ca mi
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10R1_13785-13806
-Hs Expect: Xc sutca cc trnh t tng
ng. E cng nh chiu cng cao.
- Score v Excpect t lnghch.
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Ti liu chia s ti: wWw.SinhHoc.edu.vnPrimer blast-Chc nng nh gi mi
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Kt qu phn ng nh gi mi
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Ti liu chia s ti: wWw.SinhHoc.edu.vnCch ng k trnh t c gii m qua
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Cch ng k trnh t c gii m quaphn mm sequin
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ChnGenebank
(NCBI)
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Bc 1: in thng tin
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