Jessica Dantzer
Mooney Lab
Center for Computational Biology and Bioinformatics
Indiana University School of Medicine
Automated analysis of viral integration sites in gene therapy Automated analysis of viral integration sites in gene therapy research using the SeqMap web resourceresearch using the SeqMap web resource
• CCBB– Sean D. Mooney– Xiaoman Li– Brandon Peters
• Herman B Wells Center for Pediatric Research– Sara Dirscherl– Mary C. Dinauer
• Department of Medical & Molecular Genetics– Scott Cross– Kenneth Cornetta
• Others– Robert Getty– Susan Jean Johns– Giselle Knudsen
Funding provided by: • P01HL53586 (PI: Dinauer) • K22LM009135 (PI: Mooney)• The Indiana University Vector Production Facility• INGEN.
The Indiana University Vector Production Facility is a NIH designated National Gene Vector Laboratory (U42RR11148, PI: Cornetta).
The Indiana Genomics Initiative (INGEN) is funded in part by a grant from the Lilly Endowment.
Acknowledgements Support
What is gene therapy?• Cells affected by diseases caused by “broken” genes can be aided
by the addition of “working” genes
• Working genes are carried by a vector, in most cases an altered virus, into an individual’s cells
• Working genes which are incorporated into the cell then produce their protein products, correcting the cell’s malfunction
• So cells will continue to create copies of inserted DNA, new gene needs to integrate into cell’s DNA– Altered retroviruses (disease-causing genetic material removed)
integrate their genetic material into the host organism’s
• Insertional mutagenesis-- Insertion disrupts DNA sequence and causes changes in nearby gene expression
WorkflowSeqMap Workflow
SeqMap: an automated tool
• Genes within 300kbp of the integration site are found using BLAT (BLAST-like alignment tool) on local copies of UCSC and Ensembl
• Functional analysis is provided by noting genes found in the Retroviral Tagged Cancer Gene Database (RTCGD) and associated Gene Ontology (GO) terms
SeqMap: genome mapping
Future
• Collect publicly available gene therapy data and incorporate into site– Working with Indiana University Vector
Production Facility to collect data
• Future additions to the SeqMap tool also include– analysis of functional annotations
