Introduction to Instruments
FUJI DRI-CHEM 4000i
Scil Vet Focus 5 Cryostat for Frozen Sections
2010/12/06
Speaker : Le-Shi Tsai
Notice for the use of animal center Instrument
• Please attend illustration meeting before you begin operating instruments. If you do not participate in the illustration meeting , you may NOT use the instrument. (The meeting will be held 2-3 times per semester, and please pay attention to the animal center announcement.)
• Please fill the registration forms 1 or 2 days prior to use the instrument to facilitate preparation.
Charging methods
Instrument Charging methods
FUJI DRI-CHEM 4000iBiochemistry Analyzer
*Less than 3 tests :First hour - $ 400/hour
Additional hours-$ 200 /hour
*More than 3 tests :Each one more test, the first hour charge extra $100
Scil Vet Focus 5 Hematology Analyzer
1 test - $750
each additional test -$100
Leica CM3050 S Cryostat for Frozen Sections
$150/0.5hr
FUJI DRI-CHEM 4000i Biochemistry Analyzer
Main instrument
1. The registration forms 2. Exception code table
Need to purchase reagents by user
Colorimetric
Potentiometric Linear Measurement time
External Structure of the Instrument
廢料盒
Can not open the lid when indication light is on
Light source
Internal Structure of the Instrument
Automatic detection
1. can be automatically diluted 2. A test only needs at least 10μL.( Manual detection )
Principle: Colorimetric
Spreading layer:1.filtered out macromolecules (proteins, dyes, etc.)2.evenly spread the fluid sample into the reaction layer
β-D-glucose + O2 + H2O GOD D-gluconic acid+ H2O2
1,7-Dihydroxynaphthalene + 4-aminoantipyrine +H2O2 POD Red dye
505nm
reflection density calibration curve glucose concentration
Principle: Potentiometric
generated voltage difference between ion selective electrodes applied to a reference solution and to the sample
potentiometric difference between the sample and the reference solution is measured
E = E0 + 2.303 * RT/Nf * Log aiai = f * CiCi = Ion concentration
Scil Vet Focus 5 Hematology Analyzer
Introduction to Instrument
• The following animals can be detected: dog 、 rat 、 pig 、 rabbit 、 mouse 、
G. pig
• The following items can be detected: WBC 、 DC 、 RBC 、 HGB 、 HCT 、 MCV 、 MCH 、 MCHC 、 RDW 、 Retics 、PLT 、 MPV 、 PCT
Slide 002
Equipment
main Instrument
printer
the kit
waste pail
the registration forms
Equipment
the kit
1.Diluent2.Clean Deproteinizer3.Cellyse
1. red blood cell lysis 2. reagent to convert all hemoglobin to the stable cyanmethemoglobin form
Principles
感應區
Diluent form a vortex
Cells are injected into the core of a sheath flow and confined to a narrow single-file stream by hydrodynamic focusing(15μm).
The cells are forced to pass through aperture in single file (one by one).
1. A distinguishing feature of blood cell types is
the diameter of the cells.( RBC 、 WBC 、 Platelet)
2. WBCs are classified according to the characteristics of their cytoplasm and nucleus.3. Cyanmethemoglobin can then be detected by a spectrophotometer set at 540 nm. The concentration of hemoglobin is then calculated from the optical density of the solution.
Specimen preparationSpecimen requirements
1. Use the EDTA anticoagulant 3 ml tubes.
2. Specimen volume: At least 1.5 ml
3. Specimen volume are less than 1.5mL(at least 100 μL):
Use small test tube containing a specimen and add
EDTA as an anti-coagulant (Manual mode).Preservation conditions
Samples can be analyzed within 4 hour(room temperature).
Interfering factors
Measurement results are affected by hemolysis, chyle and clotted blood.
DO NOT use clotted blood specimen.
Automatic Mode
• open the lid
• mix the specimen well
• put your sample into the
specimen- container
housing rack
Leica CM3050 S Cryostat for Frozen Sections
Frozen Tissue Processing
only use O.C.T as embedding medium
Hard tissue using a dedicated film
tissue process and cutting
fixing and embedding
freeze and slice
stain
mounting
observe it under a microscope
The advantage of Leica CM3050 S • It is ideal for undecalcified hard tissue :
1. can be set to any section thickness and
section automatically
2. with a dedicated film, can avoid the hard
tissue broken.• with rough-cut function• adjustable specimen temperature : In accordance with the type of specimen to select the appropriate
temperature.• adjustable cutting speed
Specifications
Specimen size Maximum 40mm
Section thickness setting 0.5~300μmSectioning speed 0.1~210mm/sSpecimen temperature -10 ~-50 ℃ ℃
supplies you need to purchase
Consumables Suitable specimens
Stage Buy different size according to the size of the specimen
Disposable blades General tissue
Ukrainian carbon blades
Hard tissue
Dedicated film Hard tissue
Thanks for your attention