![Page 1: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/1.jpg)
Installing BioLinux on Mac OS X or Windowsusing a virtual machine
Dr. Habil Zare, PhD
![Page 2: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/2.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
2
Bio-Linux
Bio-Linux is a free workstation platform that facilitates running hundreds of bioinformatics tools without the corresponding installation hassles.
These guidelines will help you installing Bio-Linux from the provided flash drive on your Mac or Windows computer.
![Page 3: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/3.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
3
Requirements
- A computer with at least 20 GB available space on the hard drive. A minimum of 4 GB of RAM is needed.
- The installation can take 10-20 minutes excluding the download time.
![Page 4: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/4.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
4
USB flash driveTo save time on installation, a USB drive is provided for the workshop. It contains the following files:
1. VirtualBox-5.0.8-103449-OSX.dmghttp://download.virtualbox.org/virtualbox/5.0.8/VirtualBox-5.0.8-103449-OSX.dmg
2. VirtualBox-5.0.8-103449-Win.exehttp://download.virtualbox.org/virtualbox/5.0.8/VirtualBox-5.0.8-103449-Win.exe
3. bio-linux-8-latest.ovahttp://nebc.nerc.ac.uk/downloads/bio-linux-8-latest.ova
Alternatively, you can download these files from VirtualBox and Bio-Linux, which may take several hours.
![Page 5: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/5.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
5
Step 0: Close all your programs.
BioLinux needs extensive memory and CPU resources. Close all other programs unless you have a fast, high-performance computer.
![Page 6: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/6.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
6
Step 1: Install VirtualBox Mac OS X
![Page 7: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/7.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
7
Step 1: Install VirtualBox Windows
![Page 8: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/8.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
8
Step 1: Install VirtualBox Mac OS X Windows
![Page 9: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/9.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
9
Step 1: Install VirtualBox Mac OS X Windows
![Page 10: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/10.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
10
Step 1: Install VirtualBox Mac OS X Windows
![Page 11: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/11.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
11
Step 1: Install VirtualBox Mac OS X Windows
![Page 12: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/12.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
12
Step 1: Install VirtualBox Mac OS X Windows
![Page 13: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/13.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
13
Step 1: Install VirtualBox Mac OS X Windows
![Page 14: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/14.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
14
Step 2: Run VirtualBox Mac OS X
![Page 15: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/15.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
15
Step 2: Run VirtualBox The first time you run VirtuaBox, it looks like this:
![Page 16: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/16.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
16
Step 3: Import BioLinuxMac OS X Windows
From the top menu, click on File>Import Appliance…
![Page 17: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/17.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
17
Step 3: Import BioLinuxMac OS X Windows
![Page 18: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/18.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
18
Step 3: Import BioLinuxMac OS X Windows
Use the bio-linux-8-latest.ova file from the USB drive.
![Page 19: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/19.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
19
Step 3: Import BioLinuxMac OS X Windows
Make sure, in the next step, you will check
Reinitialize MAC address for all network cards.
Both Mac OS X and Windows
![Page 20: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/20.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
20
Step 3: Import BioLinuxMac OS X Windows
![Page 21: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/21.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
21
Step 3: Import BioLinuxMac OS X Windows
It can take 10-20 minutes ….
![Page 22: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/22.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
22
Step 4: Turn on BioLinuxMac OS X Windows
Double-click on Bio-Linux to turn it on.
![Page 23: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/23.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
23
Step 4.5: Enable VT-xWindows only
In Windows, you may get an error like this the first time you run Bio-Linux. To fix it, you need to enable Virtualization Technology (VT-x) by modifying BIOS, which is a little technical. Restart while pressing F2, F10, F12, or DEL.
![Page 24: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/24.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
24
Step 4: Welcome to Linux world!Bio-Linux should appear after a black screen.
From this point on, there is not much difference between Mac OS X and Windows.
![Page 25: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/25.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
25
Step 4: Welcome to Linux world!Drag the bottom right corner of the screen to adjust
it to your convenience.
![Page 26: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/26.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
26
You are done!
- Bio-Linux is installed, and should work properly.
- You do not need the USB driver any more.
- Note that the above approach uses a virtual machine (VirtualBox), which is appropriate only for educational purposes and running simple tests by beginners. For more complicated analysis, installing Bio-Linux on a flash or hard drive is recommended to increase computation speed.
![Page 27: Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD](https://reader035.vdocuments.site/reader035/viewer/2022081503/5697bfeb1a28abf838cb80e9/html5/thumbnails/27.jpg)
Instaling BioLinux using VM, Dr. Habil Zare 27 Oct 2015
27
References:
• These guidelines were based on a blog post by Dave Lunt. http://www.davelunt.net/evophylo/2011/07/bio-linux-in-a-virtualbox/
• See the Bio-Linux website for documentation, training, and the list of supported software.http://environmentalomics.org/bio-linux/
• I prepared these guidelines to facilitate the “Bioinformatics for biologists workshop” to be held on 20 Nov 2015, UTHSC – San Antonio.http://oncinfo.org/Bioinformatics+for+biologist+workshop