Improved Design for Improved Design for Fine-Needle Aspiration (FNA) of Fine-Needle Aspiration (FNA) of
Breast Cancer Lesions Breast Cancer Lesions Alissa GarmanAlissa Garman
Janie GoldsworthyJanie GoldsworthyKristi HinnerKristi HinnerNick KortanNick Kortan
Client: Elizabeth BurnsideClient: Elizabeth BurnsideAdvisor: John WebsterAdvisor: John Webster
Final Presentation May 2, 2003Final Presentation May 2, 2003
Problem StatementProblem Statement
GOAL:GOAL: To modify the needle used during a To modify the needle used during a Fine Needle Aspiration (FNA) procedure. Fine Needle Aspiration (FNA) procedure. The modification should not drastically The modification should not drastically change the FNA procedure and still change the FNA procedure and still increase the amount of tissue removed for increase the amount of tissue removed for cytological testing. It should also minimize cytological testing. It should also minimize the time (by collecting an adequate sample the time (by collecting an adequate sample size on the first attempt) and discomfort size on the first attempt) and discomfort caused to the patient during the procedurecaused to the patient during the procedure. .
What is FNA?What is FNA?
Technique used to Technique used to extract cells; no extract cells; no incision neededincision neededSeveral insertions are Several insertions are usually required to usually required to ensure an adequate ensure an adequate sample sizesample sizeSamples are sent to Samples are sent to pathologist to be pathologist to be analyzedanalyzed National Breast Cancer Center,
http://www.NBCC.org
When does FNA not work?When does FNA not work?
Approximately 15% of trialsApproximately 15% of trials
– Dense, fibrotic, lobular tissue masses Dense, fibrotic, lobular tissue masses can prevent sufficient sample from can prevent sufficient sample from conventional techniqueconventional technique
Drill bit design could be as a backup in Drill bit design could be as a backup in such casessuch cases
Fine Needle Aspiration (FNA)
A.D.A.M., http://www.adam.com
Product Design SpecificationsProduct Design Specifications
Removes adequate number of cells for Removes adequate number of cells for diagnosis.diagnosis.
Should not cause more discomfort than Should not cause more discomfort than current FNA procedure.current FNA procedure.
Constrained to standard 20-25-gauge Constrained to standard 20-25-gauge needle, client prefers 23-gauge.needle, client prefers 23-gauge.
Current PrototypeCurrent Prototype
Pre-manufactured drill bitPre-manufactured drill bit
6” length6” length
Fits in 23 gauge needleFits in 23 gauge needle
Tissues TestedTissues Tested
Fixed cat tissues (previous semester)Fixed cat tissues (previous semester)
– MammaryMammary
– LymphLymph
– FatFat
Living Tissues (this semester)Living Tissues (this semester)
– Mouse cystic and solid tumorsMouse cystic and solid tumors
Testing ProceduresTesting Procedures
Old techniqueOld technique
– No insert usedNo insert used
– Performed multiple trials on all tissuesPerformed multiple trials on all tissues
New techniqueNew technique
– Introducer followed by drill bitIntroducer followed by drill bit
– Performed multiple trials on all tissuesPerformed multiple trials on all tissues
Sample QuantificationSample Quantification
Old techniqueOld technique– 15 % of trials resulted in either a mass of 15 % of trials resulted in either a mass of
cells or a minimal quantity of cells cells or a minimal quantity of cells
New techniqueNew technique– More dispersed cell sampleMore dispersed cell sample– Similar number of cells on averageSimilar number of cells on average– May be easier technique for novicesMay be easier technique for novices– May be used as back-up to conventional May be used as back-up to conventional
techniquetechnique
Future WorkFuture Work
Further prototype developmentFurther prototype developmentResearch into appropriate materials to be Research into appropriate materials to be used (stainless steel, Co-Cr, Ti)used (stainless steel, Co-Cr, Ti)Develop protocol for quantifying testingDevelop protocol for quantifying testingTest on freshly removed fibrotic Test on freshly removed fibrotic (pancreatic) tissue(pancreatic) tissueHuman trialsHuman trialsCytological evaluationCytological evaluationJournal publicationJournal publication
Sample SlidesSample Slides
Benign FNA Cell Sample
Sample SlidesSample Slides
Malignant FNA Cell Samples
Drill Bit Size ComparisonDrill Bit Size Comparison