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Page 1: Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

Human cells stained with an antibody for the Tubulin protein

Image from Molecular Probes

Page 2: Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

GFP-RAS2 in living Saccharomyces cerevisiae

Page 3: Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

Images from Roger Tsein’s Lab

Page 4: Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes
Page 5: Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

Making a Fusion Protein:

Yak1 Gene:DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

Protein: N-Met-Asn-Gln-…-Thr-Ser-C

GFP Gene:DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

Protein: N-Met-Ser-Lys-… -Leu-Lys-C

Yak1

GFP

Page 6: Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

Making a Fusion Protein:

Yak1 Gene:DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

GFP Gene:DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

Fusion Gene: AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...

Page 7: Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

Making a Fusion Protein:

Yak1 Gene:DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

Protein: N-Met-Asn-Gln-…-Thr-Ser-C

GFP Gene:DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

Protein: N-Met-Ser-Lys-… -Leu-Lys-C

Fusion Gene: AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...

Fusion Protein:N-Met-Asn-Gln-…-Thr-Ser-Met-Ser-Lys-… -Leu-Lys-C

Yak1

GFP

N

C

Page 8: Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes
Page 9: Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

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