HRS-10
Biomimetic peptidecombined with a red clover extract-For stronger andthicker hair
HAIR GROWTH CYCLEThe importance of hair in our lives cannot be overstated. Whether men or women lose their hair, they lose much more than their natural, youthful appearance. People also lose their self-esteem and self-confidence associated with healthy looking hair. Hair loss also, called alopecia, is a common problem affecting both men and women.
Normal hair follicle is surrounded by healthy connective tissue and dermal papilla, and has minimal or absence of inflammation nor oxidative stress. Hair loss sufferers will most of the time have the hair growth cycle disrupted by an increase of dihydrotestosterone (DHT) hormone, loss of connective tissue integrity surrounding the hair follicle, and an increase of the inflammatory process and oxidative stress in the scalp. With age, the decrease number and size of hair follicles in the scalp will also lead to thinner hair and an increase in hair loss.
SYNERGISTIC ACTION OF A PEPTIDE AND RED CLOVER EXTRACTHRS-10 is a biomimetic peptide (acetyl tetrapeptide-3) combined with a red clover extract rich in Biochanin A. HRS-10 efficacy is based on the association and synergistic action of its two ingredients.
HRS-10 has a direct action on 5-a -reductase which modulates dihydrotestosterone (DHT) to help prevent hair miniaturization. It improves ECM proteins integrity in dermal papilla for a better hair anchoring and reduces damages caused by inflammation, providing fuller, thicker and healthier looking hair.
NORMAL HA IR GROWTH CYCLE
A LTERED HA IR GROWTH CYCLEe A N A GEN + a TELOGEN HAIR LOSS
> No hormonal dysfunction> ECM proteins integrity> No i mmation
> a DHT: Shorthens anagen phase Miniaturization of follicles> Loss of ECM proteins renewal Follicle size reduction and loss of hair anchoring> mmation Disruption of hair cycle
F OLLICLE
> Inhibition of 5-a-reductase, thus modulating the conversion of testosterone to DHT> ECM proteins renewal> Cytokine reduction
> Limits miniaturization of follicles> Better hair anchoring> Normal hair cycle
HRS-10 BENEFITS H A IR LOSS HRS-10 EFFICACY
INFLAMMATAA IO N REDUCT IO N
DTDD H MO DULA T ION
ECM R E NE W AL AND ANC HORINGPROTOO E INS STI MULATIOAA N
a A N A G EN e T ELOGEN a H A IR DENSI T Y
L IMITS FO L L IC L EMINIATURIAA ZATIOAA N
B ETTTT ER HAIR ANCHORING
H AIR LOOKS T HICKER
S HEATAA H
F OLLICLE MINIATAA URIZATAA ION
IMPROPER ANCHORIN G
H AIR T HINNIG
N O MORE FOLLICLE
HRS-10
TARGETTING THE CAUSES OF HAIR LOSS
Inflammation reductionIL-1a mimics the inflamation process in human fibroblasts and induces IL-8, a pro-inflamatory cytokine.
HRS-10 helps decrease pro-i mmatory cytokines with a synergistic action compared to red clover extract alone.
DHT modulation via 5- a -reductase activityDHT is formed by the action of 5- a -reductase on testosterone. DHT causes hair loss by shortening the growth cycle, producing progressively shorter and finer hair. This is the study of the capacity of Biochanin A to inhibit the 5-a reductase activity in comparison with a well known 5- a reductase inhibitor EGCG (epigallocatechin gallate a potent antioxidant found in tea).
Biochanin A inhibits 5- -reductase activity.
ECM renewal and anchoring improvementHair follicles size is determined by: - T he volume of its dermal papilla; - T he volume of the extracellular matrix.
Healthy dermal papilla will produce good ECM proteins such as collagen type III and anchoring fibers such as laminin and collagen VII which will favor a good hair anchoring in the bulb surrounding tissue. If improper ECM renewal, hair will eventually lack vigor and will thin. Cycle after cycle, the follicle becomes smaller and finally, miniaturized and fall.
Proline is an amino acid constituent of collagen, therefore it represents a good indication of newly synthesized collagen.
Acetyl tetrapeptide-3 stimulates collagen production for better ECM integrity leading to a better anchoring.
Untreated Acetyl tetrapeptide-30
2
4
6
8
10+35 %
OH
-Pro
line
(μ
g/m
L)
Control IL-1α IL-1α +DMS1μM
IL-1α +Red clover
0.5% 1%
IL-1α +HRS-10
0.5% 1%
050
100150
20025030035 0400450 -17% -11% -20% -33% -48%
IL-8
(pg/
mL)
IL - 8 PRODUCTION BY FIBROBL A STS
Control EGCG 100μM
TY
PE
1
TY
PE
2
TY
PE
1
TY
PE
2
Biochanin A 100μM0
25
50
75
100+17,2% -93 %-64%-5 %-11%
5-α
redu
ctas
e ac
tivity
(%)
INHIBITION OF 5-a - REDUCTA SE
H YDROXYPROLINE DOSA GE IN FIBROBLA STS
Quantification of laminin, type III collagen synthesis induced by Acetyl tetrapeptide-3 on fibroblasts. It has been demonstrated that molecules which have an activity on these ECM proteins stimulation, also increase hair follicle size and improve hair anchoring.
Acetyl tetrapeptide-3 stimulates dermal papilla extracellular matrix proteins, and thus has a direct effect on structures surrounding hair follicles.
Collagen VII is a major constituent of the anchoring fibrils and is located in the basement membrane (DEJ) around the papilla. In this experiment, corticoids are used to induce a decrease in the hair papilla cells growth and ECM protein.
Acetyl tetrapeptide-3 provides a repairing effect at the dermal-epidermal junction level, helping hair anchoring.
IMPROVING HAIR FOLLICLE LENGTHHuman hair follicles in anagen phase are isolated from skin biopsys and cultured with or without Acetyl tetrapeptide-3 for 8 days.
Acetyl tetrapeptide-3 improves by 35% the length in comparison with untreated areas after 8 days of treatment.
Control(untreated)
+ Corticoïds Corticoïds+ Acetyl
tetrapeptide-3
0
1
2
3
4
70 %
Scor
e
Q UA NTIT ATIVE EV A LU ATION OF COLL A GEN VII
Type III Collagen Laminin0
20
40
60
80+285 %
+65 %
Fluo
resc
ence
inte
nsity
Untreated Acetyl tetrapeptide-3
S TIMUL ATION OF ECM PROTEINS SYNTHESIS BY FIBROBL A STS
Day 0 Day 80
500
1000
1500
2000
2500
3000
3500
4000+35 %
Folli
cle
elon
gatio
n (μ
m)
Untreated Acetyl tetrapeptide-3 (0.0006% HRS-10)
ME A SUREMENT OF HA IR LENGTH
Control + Acetyl tetrapeptide-3
Type III Collagen
Laminin
F IBROBL A STS
Skin + Corticoïds
Normal skin (untreated)
Skin + Corticoïds + Acetyl tetrapeptide-3
Collagen VII
DEJDermis
SC
Epidermis
PROVIDING FULLER AND THICKER LOOKING HAIR Test protocol
- 30 men with androgenetic alopecia: 15 treated with the HRS-10 lotion (5%) and 15 with a placebo.
- Daily application of 20 drops of the HRS-10 lotion or placebo for a period of 4 months.- A digital trichogram (TrichoScan professional) was taken at D0 and 4 months later.
HRS-10 induces a visible increase in the anagen hair density in comparison with placebo.
HRS-10 induces a strong reduction in the telogen hair density in comparison with placebo.
A/T ratio = Comparison of the number of anagen and telogen hair, which is an indication of the percentage of active hair follicles.
HRS-10 increases the A/T ratio by 46% compared to a reduction of -33% for the placebo.
Green: anagen Red: telogen
Before treatment
After treatment
a anagen hair e telogen hair
Day 0 4 months Day 0
Placebo HRS-10
4 months80
84
88
92
96
100
104 +13%
-2 %
# of
hai
rs/c
m2
Day 0 4 months Day 0
Placebo HRS-10
4 months0
10
20
30
40
50
60
70
-29 %
+23 %
# of
hai
rs/c
m2
A VER A GE A N A GEN HA IR DENSITY
A VER A GE TELOGEN HA IR DENSITY
Day 0 4 months Day 0
Placebo HRS-10
4 months0
1
2
3
4+46% *
-33%
Ana
gen/
Telo
gen
ratio
A N A GEN / TELOGEN R ATIO
* p<0.05
HRS-10