PURDUERESEARCH PARK
KPI: Kuiwon Choi (KIST)FPI: James F. Leary (Purdue Univ.)
Molecular Imaging and Nanomedicine for Theragnosis
using Nano-Biomaterials
Global Research Lab Program
PURDUERESEARCH PARK
Global Research Lab (GRL) The Global Research Laboratory (GRL) program was initiated by the Korea Foundation for International Cooperation of Science and Technology (KICOS) in 2006.
The GRL program has been designed to develop fundamental and original technologies through international collaborative research between Korean and foreign laboratories.
A proposal submitted for the GRL program should address a research topic which requires Korean and foreign research partners to engage in close collaboration for the attainment of significant scientific and technological goals.
PURDUERESEARCH PARK
Goals of GRL Program
GlobalResearch
Leader in KOREA
GlobalResearch
Lab. In KOREA
GlobalResearch Leader Networking
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August, 20051st MOU between Biomedical Research Center (KIST) and Bindley Bioscience Center (Purdue)
September 7, 20061st Purdue-KIST collaborative symposium, entitled “Molecular Imaging and Nanomedicine”, was held at KIST.
March, 20072nd MOU between Life Science Division (KIST) and Bindley Biosciences Center (Purdue)
September, 20072nd Kist-Purdue collaborative symposium at Purdue Univ.
History of KIST-Purdue Univ. Collaboration
Dr. James F. Leary Dr. Kinam ParkDr. J. Paul Robinson Dr. Ji-Xin Cheng
PURDUERESEARCH PARK
Results of the Collaboration
Have been collaborating on joint researches for past 5 years
Successful scientist exchange: Seven young scientists from KIST joined Bindley Bioscience Center as post-doctoral fellows.
Some of them came back to Korea; Dr. J.H. Park (Kyung Hee Univ.) Dr. Y.W. Cho (Hanyang Univ.) Dr. K.M. Huh (Chungnam National Univ.) Dr. S.C. Lee (Korea Inst. of Ceramic Eng.&Tech.)
Recently, Dr. E.A. Kang (Purdue Univ.) from Purdue Univ. joined molecular imaging team in KIST as Post-Doc.
Publication activity between two labs: 10 papers, 7 book chapters
PURDUERESEARCH PARK
KPI: Kuiwon Choi (KIST)FPI: James F. Leary (Purdue Univ.)
Molecular Imaging and Nanomedicinefor Theragnosis using Nano-
Biomaterials
KIST-Purdue GRL Program
PURDUERESEARCH PARK
KIST-Purdue GRL Program
To develop original & fundamental technologies of “Theragnosis” throughKIST-Purdue complementary researchcollaborations.
To commercialize the technologiesthrough global network based on GRL.
To upgrade the KIST-Purdue GRL to the world class research center
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Process and Result 7 GRLs were started in 2006.
5 GRLs were selected in 2007.
- Preliminary proposal was submitted in March.
- 4 proposals passed the 1st round of evaluation out of 20 proposals in BT area.
- 2nd round of evaluation (oral presentation for 1 hr.)was held in May.
Prof. Leary gave a great presentation despite ofhis severe backpain!
- The final 1 proposal was selected in May.
PURDUERESEARCH PARK
Future Medicine: TheragnosisTheragnosis : Therapy + DiagnosisNanomedicine and molecular imaging could be a strategic initiative to combine clinical and basic researches into a directed application toward theragnosis
Molecular ImagingMedical Information from imaging modality
Nanomedicine
Chemical drugDNA & ProteinImaging agents
PURDUERESEARCH PARK
Innovative Nano-Bio materials for Theragnosis
Biomarker for target cell
Cancer drugsEngineered genesTherapeutic proteins Photo sensitizer
Tracking optical probesPET or MR imaging probesSmart optical probes • Biological mechanism
• Live cell imaging• Live animal imaging• Drug delivery system• Engineered cell therapy• Early diagnostics• Drug screening in vivo• Targeted therapy
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Molecular Imaging
- Smart probes - Live cell imaging- Early diagnosis- High throughput drug screening - New imaging system
<2003, Technical Review, MIT>
cm mm μm nm
organ tissue cell DNA, protein
Molecular imaging is defined as the measurement and/or imaging of biological processes in living organism at the molecular and cellular level
PURDUERESEARCH PARK
- Nano-sized drug delivery- Molecular diagnosis- Nano biomaterials- Regenerative nanomedicine- Nano-scaled therapy
NanomedicineNanomedicine is the application of nanotechnology to the prevention and treatment of diseases in the human body
<2006, Technical Review, MIT>
PURDUERESEARCH PARK
Theragnosis in NTRM
Portfolio Analysis of Strategic Products and Functions Aiming at Bio-Healthtopia (NTRM, Vision II)
Eco
no
mic
Im
pact
Strategic Importance
very low low middle high very highvery low
low
middle
high
very high
Cell therapy
Prognostic systemvaccines
pulmonary
immune system
gene therapy
Cardivascular
diagnostics
anticancer drug
Rehabilitation system
medical imaging system
Nanomedicine
Molecular Imaging+
Theragnosis
PURDUERESEARCH PARK
Structure of GRL Program The collaboration between the KIST Biomedical Research Center and the Purdue Bindley Bioscience Center will be the most promising implement for theragnosis.
This research program will be the first program in the world which integrates molecular imaging and nanomedicine.
KIST Purdue Univ.Molecular Imaging
Nanomedicine
TheragnosisSmart imaging probe
Tumor targeting drug delivery
Fusion protein for cell imaging
Fluorescence optical imaging
Infrared imaging & therapy
Nano-factory for cell engineering
Drug delivery system for nanomedicine
Cytomics for nanomedicine
Nano-therapy and treatment
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Status of Korean Research Lab
Principle Investigator Research Field
Original Technology Results
Dr. Kuiwon ChoiDr. Inchan Youn
New imaging modality
- Imaging software for imaging analysis- Infrared imaging modality- Microwave thermo-therapy
- paper; 76- patent; 15
Dr. Ick Chan KwonDr. Kwangmeyung Kim
Optical imaging
- Nano-sized drug delivery - Smart polymer-based probes
- paper; 110- patent; 45
Dr. Hyesung JeonDr. Hyungjun Ahn
Cellular imaging
- Reporter gene preparation - Live cell imaging with FRET technology - Electron Microscopy imaging
- paper; 30- patent; 5
Dr. Kuiwon Choi: The Korean Society of Biomechanics, Vice PresidentDr. Ick Chan Kwon: Korean Molecular Imaging Society, Vice President
Journal of controlled release (IF=3.696), Asian Editor
Structure and physiological function of the low density lipoprotein Receptor, Annual Review of Biochemistry (2005, IF=33.456)Polymeric Nanoparticles for Apoptosis Imaging, JACS (2006, IF=7.419)Polymeric nanoparticles for protein kinase activity, Chem Com (2007, IF = 4.426)Biopolymers for molecular imaging, Progress in Polymer Science (2007, IF=16.042)Protein phosphorylation-responsive polymeric nanoparticlesfor imaging protein kinase activities, Angewandte Chemie (2007, IF=9.956)
PURDUERESEARCH PARK
Status of Korean Research Lab KIST Molecular Imaging Lab
KIST Regional Lab (Kyungbuk Medical School)
KIST Regional Lab (Asan Medical Center)
Principle Researcher: 2Senior Researcher: 4Post-Doc: 4Student: 9Researcher & Staff: 8
Near-infrared Microscopy
Fluorescent Microscopy
Imaging Box
Optical Tomography
ConfocalMicroscopy
Prof. Sangyun Kim, MDStaff: 5
Prof. In San Kim, MDStaff: 3
micro-PETAnimal room Clinical test Cyclotron for isotope labeling
PURDUERESEARCH PARK
Status of Foreign Research Lab
Principle Investigator
Research Field Original Technology Results
Dr. J. F. Leary - Nanomedicine- Regeneration nanomedicine- cancer treatment
- paper; 88- patent; 7
Dr. Kinam Park - Drug delivery system - Drug delivery system- Nanomedicine
- paper; 160- patent; 25
Dr. J. P. Robinson Dr. Ji-Xin Cheng
- Applied Cytomics- New imaging modality
- Cytomics- CARS imaging modality- Drug delivery system
- paper; 140- patent; 10
Dr. J. F. Leary: Purdue SVM Endowed Professor of NanomedicineFellow of the American Institute for Medical and Biological Engineering
Dr. Kinam Park: Showalter Distinguished Professor of Biomedical EngineeringJournal of controlled release, Editor-in-chief
Dr. J. P. Robinson: Current Protocols in Cytometry, Editor-in-chiefInternational Society for Analytical Cytology, President
Evaluation of disulfide reduction during receptor-medicated endocytosis by using FRET
imaging, Proceeding of the National Academy of Science (PNAS) (2006, IF=10.231)In situ sisualization of paclitaxel distribution and release by coherent anti-Stokes Ramanscattering microscopy, Anal Chem (2006, IF = 5.635)Mechanisms of controlled drug release from drug-eluting stents, Advanced Drug DeliveryRevies (2006, IF=7.189)In vitro and in vivo two-photon luminescence imaging of single gold nanorods, Proceeding ofthe National Academy of Science (PNAS) (2005, IF=10.231)
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Status of Foreign Research Lab Cover papers Books
World-wide software distributionMedia
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Purdue BBC
Birck Nanotechnology CenterBiomedical Engineering
Professor: 4Post-Doc: 3Student: 6Researcher & Staff: 5
Bindley Bioscience Center (BBC)Bindley Bioscience Center (BBC)
Cytometry Instruments (Opto-injector, Cell sorter & Flow cytometry)
Confocal Microscope
Clean RoomMulti-photonConfocal Microscope
Coherent Anti-Stokes Raman Scattering Imaging BioMEMS
Status of Foreign Research Lab
PURDUERESEARCH PARK
1. Nano-Bio dot for microwave thermotherapy
Research Contents in KRL
Gold nanoparticles
normal SCC7tumor
Near IR Thermo MRI
Far IR thermo imaging
Diagnostic imaging
Microwave
Targeted MicrowaveThermotherapy
Thermomonitoring
MMP’s
• Near IR
• Thermo MRI
• Far IR thermo
PURDUERESEARCH PARK
100μm100μm
30 min30 min
Polymeric nanoparticle
Target molecule
Apoptosis & Protein Kinases Imaging Tumor Imaging OA & RA Imaging
2. Nano-polymeric particle smart probes for optical imaging
3. Nano-hybrid fusion protein for cellular imaging
Nuclear shuttling by fusion protein
Receptor-ligandinteraction imaging
Research Contents in KRL
PURDUERESEARCH PARK
1. Nano-factory for nanomedicine
Novel therapeutic gene regulation by genetic biosensor tethered to magnetic nanoparticles for the detection and treatment of retinopathy of prematurity
2. Nano-sized drug carriers for nanomedicine
Nano-sized drug carriers for tumor targeting
14day 15day 16day 17day9day 11day 12day 13day
0min 1day 2day 3day 4day 6day0min 1day 2day 3day 4day 6day 7day 8day
Tumor treatment with drug-loaded nanoparticles
Research Contents in FRL
PURDUERESEARCH PARK
3. Nano-hybrid materials for cytomics
4. Nano-bio probes for non-linear optical imaging
Multi-waved fluorescence materials for cell imaging and isolation
Development of Coherent Anti-Stokes Raman Scattering (CARS) microscopy
Probing the molecular composition of lipid membrane domains
Research Contents in FRL
PURDUERESEARCH PARK
KIST: Molecular Imaging
Purdue University: Nanomedicine
Nano-Polymeric Particle Smart Probes for Fluorescence Optical Imaging Project Leaders: Dr. I.C. Kwon and Dr. K. Kim
Nano-Hybrid Fusion Proteins for Cellular ImagingProject Leaders: Dr. H. Jeonand Dr. H.J. Ahn
Nano-Bio Dot for Microwave ThermotherapyProject Leaders: Dr. K. Choiand Dr. I.C. Youn
Nano-Factory for Cell Engineering Project Leaders: Prof. James F. Leary
Nano-Sized Drug Carriers for NanomedicineProject Leaders: Prof. K. Park
Nano-Hybrid Materials for CytomicsProject Leaders: Prof. J. Paul Robinson
Nano-Bio Probes for Non-linear Optical ImagingProject Leaders: Prof. Ji-XinCheng
Nano-Bio materials for Theragnosis
- Nano-Bio probes for molecular imaging & drug delivery system
- Nano-Bio hybrid fusion proteinfor cytomics & medical imaging
- Nano-Bio dot for cancer imaging & treatments
- Nano-Bio factory for molecular imaging and cell engineering
Global Research Lab
Research Networking
PURDUERESEARCH PARK
Research Schedule for 1st and 2nd Year No. Stage I: Synthesis Nano-Bio materials for Molecular Imaging and
Nanomedicine (1st year)Month
0 6 12 18 24
Review of patents and papers in 19901Output: Reports for theragnosis
-------- -
Synthesis of nanoparticle optical probe for molecular imaging 2Output: optical probes for cancer diseases (2ea)
---------- ------------ ---------
Synthesis of nanoparticle optical probe for molecular imaging 3Output: fusion protein probes for apoptosis & protein kinases (2ea)
------------ ----------- ----------- ---------
Preparation of Nano-Bio dots for cancer imaging and therapy 4Output: gold particle-based probes (2ea)
----------- ----------- ----------
Synthesis of Nano-sized drug carriers & Nano-factor for Nanomedicine5Output: nano-sized drug carriers (2ea), Nano-factor (1ea)
------- ----------- ---------
Preparation of Nano-sized probes for cytomics & Non-linear opticalimaging
6Output: nano imaging probes for cytomics & non-linear optical imaging(3ea)
------------- ------------- ----------
No. Stage II: Evaluation of Nano-Bio materials in cell-based screening system as new imaging probe & (2nd year)
Month 0 6 12 18 24
Cell-based screening of molecular imaging probes1Output: molecule imaging probes for cellular imaging (5ea)
------------ ---------
Therapeutic efficacy measurements of nano-sized drug carriers, nanofactory, gold particles in cellular based screening
2
Output: Data sheet for therapeutic efficacy in cellular based screening
----------- ------------- ---------
Cytotoxicity of nano-bio materials for molecular imaging probes and 3Output: Data sheet for cytotoxicity of new materials
------------- ----------
PURDUERESEARCH PARK
Technical Roadmap
Smart Imaging Nano-Probes
Fusion Gene/Protein forImaging/Medicine
Multi-Func.Drug Carriers
High-Throughput Drug Screening
New Imaging System for Early Diagnosis Molecular-Based Nano-Therapy Nano-factory for
Nanomedicine
2007 2008 2009 2010 2011 2012 2013 2014 2015
Platform Tech. for Theragnosis
Innovative Fusion Tech. for Theragnosis
Applied Tech. for Theragnosis
Nano-Bio Hybrid Probes for Molecular Iamaging
Fusion Gene/Protein Synthesis
Nano-sized Drug Carriers
Nano-Factory for Nanomedicine
Nano-Dot for Nano-Therapy
New Imaging System for Theragnosis
industry
PURDUERESEARCH PARK
Strategy for GRL Program
- Set up GRL in KIST & Purdue Univ.- Nano-Bio Materials Bank- Young Scientist Exchange Program- International Symposium - Internet Meeting - Global Networking
GRL in Purdue Univ.
GRL in KIST Internet meeting
PURDUERESEARCH PARK
Current Participant Researchers
Participant researchersKIST27
2 principal scientists, 4 senior scientists, 4 postdoctoral fellows, 9 graduate students, 8 technicians
Purdue Univ.
18
4 professors, 3 postoctoral fellows, 6 graduate studuents, 5 technicians
PURDUERESEARCH PARK
Research Budget
Direct Budget (US$)
GRL Program 550,000 $/ year3 yrs + 3 yrs + 3yrs (3 stages)
KISTIntramural Fund
Funds to Purdue Univ. for GRL Research