Genetic Connectivity of Pelagic Fishes
(Katsuwonus pelamis and Scomberomorus
commerson) across the Indo-West Pacific
Alexis Jackson Alexis Jackson 1,1, Paul Barber Paul Barber 22
1. Yale University 2. Boston University, MBL1. Yale University 2. Boston University, MBL
Species of StudySpecies of Study
Katsuwonus pelamis
Scomberomorus commerson
Katsuwonus pelamis
°° °°
Katsuwonus pelamis
Scomberomorus commerson
Biogeography of Indo-West Pacific
Regional genetic differentiation of Linckia
laevigata
Collection Sites
ObjectivesObjectives
1. Investigate population structure of pelagic fishes in IWP
2. Investigate population dynamics of pelagic fish
HypothesesHypotheses1. Fishes not affected by Pleistocene
2. Limited flow to Indian and Pacific
3. K. pelamis different from Scomberomorus
MethodsMethods•Sample Size: 241
– Scomberomorus commerson (76)– Katsuwonus pelamis (165)
•Extraction Method: Chelex•PCR•ABI 377•Statistical Analysis: PAUP,
Arlequin
K. pelamis
Population Pairwise FSTs
Katsuwonus pelamisNSU NSU 2 YAP BAK JAY ACE HLM MNK RAJ SOL SOL 2
Bitung Fish
Market, N.
Sulawesi
(NSU)
0
Manado Fish Market, N. Sulawesi
(NSU2)
0.0557 0
Yapen (YAP)
0.014 0.00691 0
Biak (BAK)
0.04751 0.01359 -0.01466 0
Jayapura (JAY)
0.03538 0.02506 -0.00452 0.00828 0
Aceh (ACE)
0.00978 -0.01526 -0.01566 -0.00483 -0.00005 0
Halmahera (HLM)
0.02056 -0.00105 -0.01175 0.01468 0.01479 -0.02039 0
Manokwari (MNK)
0.00017 0.01711 -0.00244 -0.0052 0.00286 0.00355 0.00533 0
Raja Ampat (RAJ)
0.0168 0.0184 -0.03871 0.0087 -0.02969 -0.02253 -0.01867 -0.02626 0
Honiara Fish Market, Solomon Islands (SOL)
-0.02194 -0.0122 -0.02828 -0.01109 0.00709 -0.01679 -0.02522 -0.00565 -0.03554 0
Honiara Fish Market, Solomon Islands
(SOL2)
0.00234 0.01934 -0.01814 0.00469 0.02315 0.0072 -0.00665 -0.00056 -0.04747 -0.00497 0
Population Pairwise FSTs
Katsuwonus pelamis Yapen
(YAP)0 0 0
Biak (BAK)
0 0 0 0
Jayapura (JAY)
0 0 0 0 0
Aceh (ACE)
0 0 0 0 0 0
Halmahera (HLM)
0 0 0 0 0 0 0
Manokwari (MNK)
0 0 0 0 0 0 0 0
Raja Ampat (RAJ)
0 0 0 0 0 0 0 0 0
Honiara Fish Market, Solomon Islands (SOL)
0 0 0 0 0 0 0 0 0 0
Honiara Fish Market, Solomon Islands
(SOL2)
0 0 0 0 0 0 0 0 0 0 0
AMOVA Analysis
Source of Variation Sum of
squares
Percentage of
variation
Within populations 1817.585 100.41Total: 1926.898
d.f Variance
components
Among populations 10 109.313 -0.04790 Va -0.41
156 11.65119 Vb166 11.60328
Katsuwonus pelamis
Fixation Index F ST: -0.00413 Va and FST : P(rand. value > obs. value) = P(rand. value >= obs. value) = 0.73079+ -0.00423
S. commerson
Population Pairwise FSTs
Scomberomorus commerson
TK RAJ HLM JAV FLR Teluk Cenderwasih (TK)
0.00000
Raja Ampat (RAJ)
-0.02883 0.00000
Halmahera (HLM)
0.00037 -0.00935 0.00000
Java (JAV) 0.46706 *** 0.48210 *** 0.34412*** 0.00000 Flores (FLR) 0.11832 °° 0.12575 °° 0.12828 °° 0.51204 *** 0.00000
° (p<.05), °° (p<.01) ** *(p<.001)
AMOVA AnalysisScomberomorus commerson
Source of Variation
Sum of squares Percentage of variation
Among groups 68.135 4.71Among populations w/in groups
171.926 24.77
Within populations 525.768 70.51Total: 765.829
d.f Variance components
1 0.51681 Va
75 10.96525
6 2.71656 Vb
68 7.73188 Vc
Fixation Index F ST: 0.29487 Vc and FST : P(rand. value < obs. value) = 0.000001
Molecular Diversity Molecular Diversity IndicesIndicesK. pelamis
Population N Haplotype Diversity Nucleotide Diversity ?s
Bitung Fish Market, N. Sulawesi (NI) 4 1 0.035381 15.36Manado Fish Market, N. Sulawesi (NI) 14 1 0.062973 29.5585 Yapen (P) 13 1 0.056235 26.1235Biak (P) 13 1 0.053996 21.397 Aceh (WI) 16 1 0.052144 24.1403Halmahera (EI) 22 1 0.060361 25.3683 Manokwari (P) 20 1 0.054636 25.0864Raja Ampat (P) 19 1 0.059946 24.0336Honiara Fish Market, Solomon Islands 15 1 0.06513 27.3715
23.5839Honiara Fish Market, Solomon Islands 14 1 0.060709
Molecular Diversity Molecular Diversity IndicesIndices
S. commersonPopulation N Haplotype Diversity Nucleotide Diversity ?s
N. Sulawesi 1 1 - 0Jayapura 1 1 - 0 Teluk Cenderwasih 15 1 0.041503 11.6867Raja Ampat 10 1 0.032571 14.8464 Aceh 1 1 - 0Halmahera 13 1 0.047426 18.0458 Java 15 1 0.041425 16.915Flores 20 1 0.02887 14.0935
ConclusionsConclusions• Panmixia not a phenomenon for
S. commerson in IWP
• Boundaries for pelagic fish
• Commercial significance
AcknowledgementsAcknowledgementsMany thanks to the following:Many thanks to the following:• Paul BarberPaul Barber• Elizabeth JonesElizabeth Jones• Josh DrewJosh Drew• Eric CrandallEric Crandall• Timery DeBoerTimery DeBoer• Matthew SubiaMatthew Subia• Krystle ChavarriaKrystle Chavarria• Jeannie ChoiJeannie Choi• National Science Foundation National Science Foundation
and The Diversity Projectand The Diversity Project