Fridge-4oC
Pilot test 12 – 15 Nov 07
Incubator37oC
Incubator32oC
Room temperature23oC
Purchased at Chinese Medicine Store
Packs of 20 beans each
One pack per treatment
11 Nov 07
Each pack undergone their respective treatment
24 hours, started12pm
12 Nov 07
12pm
Fold into two and roll up and place along side of the cup
200mL
6cm
6.5cm
4.3cm
20 beans below the first line on the cup
200mL
6cm
6.5cm
4.3cm
Pilot test
Pilot test
Preparation of Rice water
Made a mark on the scoop (around 15mL of water)
10 grams of rice poured into a scoop.
10 g Rice1 min
Pilot test
Watering of mung beans
Pilot test
Room
Window
Desk
light
drawers
door
Placement of set-ups
Total 12 treatments with 1 set up each = Total 12 set ups
Randomised arrangement
Grown in closed drawer darkness,homogenised environment (same temperature, humidity)
Watered at around 6pm everyday (13-16 Nov 07) (sun already set hence no effect from natural light from window)
Drawer was opened for around 45 minutes for watering.
With certain light exposure (turning on of the lights in the room)
Pilot test
Ctrl-4oC
Red-4oC
White32oC
RedRT
CtrlRT
Red37oC
White37oC
Red32oC
Ctrl32oC
Ctrl37oC
WhiteRT
White-4oC
Placement of set-ups
Actual test (17 – 21 Nov 07) 5 days
Watered at around 6pm everyday for 5 days
Ctrl-4oC
Ctrl-4oC
Ctrl-4oC
Ctrl-4oC
Ctrl-4oC
Ctrl-4oC
Red-4oC
Red-4oC
Red-4oC
White32oC
White32oC
White32oC
RedRT
RedRT
RedRT
CtrlRT
CtrlRT
CtrlRT
WhiteRT
WhiteRT
WhiteRT
Red32oC
Red32oC
Red32oC
Ctrl32oC
Ctrl32oC
Ctrl32oC
White-4oC
White-4oC
White-4oC
Total 9 treatments with 3 replicates each = Total 27 set ups
Rice type / Temperature (oC) White Red Control (no rice)
-4 White/-4 Red/-4 Ctrl/-4
23 (Room temperature) White/RT Red/RT Ctrl/RT
32 White/32 Red/32 Ctrl/32
Watering process
• Room light off, Desk light on
• Small amount of light used during watering of plants
• Each drawer was opened for around 30mins
Actual test (17 – 21 Nov 07) 5 days
6pm (after sunset)Window
Desk
light
drawers
door
chai
rch
air
Data collection
• One time point• Using normal ruler, we
measure the length of the shoot and root to an accuracy of 1 d.p.
shoot
root