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Enzyme Amperometric Sensors – Glucose and Lactate
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Glucose
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Amperometric Glucose Sensor
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Electrochemical detection of glucose
• Description
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How is the type one glucose sensor working
Direction of electron flow
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Michaelis Menten - Enzyme kinetics and rates of reaction
Rate of reaction
Concentration of substrate
Vmax
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Diffusion controlled – Cottrell current– Ficks 2nd law of diffusion
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Mass transport controlled
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Lactate
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Lactate Oxidase
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Electrode
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Electrochemical detection of glucose
• Description
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Type 1 Lactate Sensor Type 2 Lactate Sensor
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Type 2 Lactate Sensor
Electrode
electrons
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Time for practical
• ONE – record a cyclic voltammogram of a glucose sensor without glucose present.
• TWO – record a cyclic voltammogram of a glucose sensor with glucose present.
• THREE - compare the two experiments and chose the lowest voltage where you think the signal is invariant with voltage.
• FOUR – From THREE above run a series of amperometric experiment at that voltage, with increasing amounts of glucose and see what happens as you go from solution to solution.
• Record the current at 5 seconds and plot current versus concentration.