clearance of compound from blood (2.64% ID at 4 h) and tissue exceptthe kidneys (27% ID at 4 h).

Conclusion: As tissue distribution studies are very important forclinical use, results suggest that 99mTc labeling of venomcan be a usefultool for in vivo studies and is an excellent approach to follow the processof biodistribution and kinetics of toxins.

Keywords: Mesobuthus eupeus, Venom, Purification, Radiolabeling,Chromatography

doi:10.1016/j.clinbiochem.2011.08.1105

Poster – [A-10-779-1]Cardioprotective effect of safranal on isoproterenol-inducedmyocardial infarction in ratMehdizadeh Royaa, Hoseinzadeh Hoseina, Khooei Alirezab, ParizadehSeyed Mohammad Rezac, Mehri SoghraaaPharmaceutical Research Center, Pharmacodynamy and ToxicologyDepartment, School of Pharmacy, Mashhad University of MedicalSciences, Mashhad, IranbDepartment of Pathology, Imam Reza Hospital, School of Medicine,Mashhad University of Medical Sciences, Mashhad, IrancBiochemistry and Nutrition Department, School of Medicine, MashhadUniversity of Medical Sciences, Mashhad, IranE-mail addresses: roya.mehdizadeh@gmail.com (M. Roya),Hosseinzadehh@mums.ac.ir (H. Hosein),khooeiAR@mums.ac.ir (K. Alireza),ParizadehMR@mums.ac.ir (P.S.M. Reza),mehris861@mums.ac.ir (M. Soghra)

Introduction: Safranal is a monoterpene aldehyde which is themajor constituent of the essential oil of saffron and responsible for thesaffron odor and aroma. In modern pharmacological studies, safranalhas exhibited radical scavenging activity in different models. Myo-cardial infarction is followed by several biochemical alteration, suchas lipid peroxidation and free radical damage, leading to qualitativeand quantitative alterations of myocardium, therefore, the aim of thisstudy was to systematically determine whether safranal exertscardioprotection in isoproterenol-induced myocardial damage.

Materials and methods: Male Wistar rats were divided into 5groups: control, isoproterenol (ISO), and safranal (0.025, 0.050,0.075 mL/kg) treatment groups. Saffranal or vehicle was injected I.P.to rats for 9 days. On days 8 and 9, the animals in ISO and safranaltreatment groups were administered with ISO (85 mg/kg, s.c.) at aninterval of 24 h. On day 10, animals were sacrificed for biochemical(MDA, creatine kinase-MB, lactate dehydrogenase) and histopatholo-gical examinations.

Results: Isoproterenol challenged animals increased thiobarbituricacid reactive substances (p<0.001), CK-MB and LDH (p<0.001).Safranal pretreatment decreased CK-MB, LDH (p<0.001) and thio-barbituric acid reactive substances (p<0.001). The grade of heartmuscle damagewas severe inmore than 70% in the ISO group. Safranaldecreased significantly the intensity of tissue damage. Safranal at0.075 mL/kg dose exhibited maximum protective effects which couldbe due tomaintenance of the redox status of the cell reinforcing its roleas an antioxidant.

Conclusion: These results suggest the protective effect of safranalon ischemic hearts by biochemical and histopathological findings.

Keywords: Safranal, Lipid peroxidation, Antioxidant, Isoproterenol,Myocardial infarction

doi:10.1016/j.clinbiochem.2011.08.1106

Poster — [A-10-784-1]Molecular evaluation of selective inducible nitric oxide synthaseinhibitor effects on zinc chloride-induced spatial memoryalterations in Morris water maze in male ratsKaveh Tabrizian, Akram Aboutorabi, Maryam Belaran,Maliheh Khosravi, Mohammad SharifzadehZabol University of Medical Sciences, Zabol, IranE-mail addresses: k_tabrzian2005@yahoo.com (K. Tabrizian),abootorabia@yahoo.com (A. Aboutorabi)

Introduction: Zinc, an essential micro-nutrient and biochemicalelement of the human body, plays structural, catalytic, and regulatoryroles in numerous physiological functions. Considerable bodies ofevidences suggest the importance of nitric oxide (NO) for synapticplasticity in several brain regions such as the hippocampus. Thecompound 1400W appears to be more potent and selective forinducible isoform of nitric oxide synthase (iNOS).

Material and methods: The interactive effects of pre-training oraladministration of zinc chloride (10, 25, and 50 mg/kg) for 14consecutive days and post-training bilateral intra-hippocampal infu-sion of 1400W (10, 50, and 100 μM/side) on spatial memory retentionin Morris water maze (MWM) were investigated. Animals weretrained for 4 days and tested 48 h after completion of training. Also, themolecular effects of these compounds on the expression of cholineacetyltransferase (ChAT) in the CA1 region of the hippocampus andmedial septal area were analyzed.

Results: Post-training bilateral intra-hippocampal infusion of1400W into the CA1 region of the hippocampus reversed zincchloride-induced spatial memory impairment and significantly in-creased ChAT protein expression.

Conclusion: Our findings showed the probable role of selectiveiNOS inhibitors in prevention of zinc chloride-induced memorydeficits by modulation of cholinergic system activity.

Keywords: Choline acetyltransferase, Zinc chloride, CA1 region,Medial septal area, Inducible nitric oxide synthase (iNOS) inhibitor

doi:10.1016/j.clinbiochem.2011.08.1107

Poster — [A-10-809-1]Evaluation of anti-glutathione S-transferase antibody indistinguishing toxigenic Aspergillus strains and nontoxigenic compartmentsTahereh Ziglaria, Abdolamir Allamehb, Mahdi Razzagh-Abyanehc

aFaculty of Veterinary, Tehran University, Tehran, IranbFaculty of Medical Sciences, Tarbiat Modares University,P.O. Box 14115-111, Tehran, IrancDepartment of Mycology, Pasteur Institute of Iran, Tehran, IranE-mail addresses: ziglari@alumni.ut.ac.ir (T. Ziglari),allameha@modares.ac.ir (A. Allameh),mrab442@yahoo.com (M. Razzagh-Abyaneh)

Introduction: It has been demonstrated that there is a positivecorrelation exists between aflatoxin production and the glutathioneS-transferase (GST) activity in toxigenic Aspergillus flavus. Ourprevious studies show that inhibition of aflatoxins in a toxigenicAspergillus parasiticus was associated with inhibition of GST activity.In this study two techniques (ELISA and Western blot) were used tocompare glutathione S-transferase activity in toxigenic and non-toxigenic Aspergillus strains. For this purpose, polyclonal antibodywas raised in rabbits against purified GST and used to develop theimmunoassay. An indirect ELISA using second antibody conjugated tohorseradish peroxidase (HRP) was developed to measure the titer ofantibody. The antibody titer in sera of the rabbit immunized with the

Abstracts S359