Download - Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering
![Page 1: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/1.jpg)
Presented by: sanchari maityM.Sc biotechnology 1st semBioengineering:
custom microbes
![Page 2: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/2.jpg)
Contents what is bioengineering?
domains of bioengineering.Genetic and biomedical engineering.
• Recombinant insulin• recombinant human growth hormone
• Recombinant interferon• Hepatitis B vaccine production
• Phage therapy
Use in bioremediation.
• Superbug• Bio-luminescence while pollutant
degradation
Use in bioprocess engineering.
• various types of expression vectors
![Page 3: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/3.jpg)
What is bioengineering?British scientist and broadcaster Heinz Wolff termed bioengineering in 1954.
Any area of biology mixed with any area of engineering in any proportion.
Principles of biolog
y
Tools of
engineering
Bioengineerin
gUseabl
e,tangibl
e,viable
pdt
![Page 4: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/4.jpg)
engineering
Analytical & synthetic
Cost,practical application
Maths,chemistry,physics,computer
scienceStructure,process,
manufacture
biology
Genome knowledge
Molecular biology
Recombinant DNA technologyMedical,
Research,
![Page 5: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/5.jpg)
genetic engineering
Cellular engineering
Bioprocess engineering
Biomedical engineering
Biomimetics
bioinformatics
![Page 6: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/6.jpg)
Biomedical engineering
• Artificial organs• Replacement joints• Artificial skin.
• Vaccine,drug,hormone production.
![Page 7: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/7.jpg)
Bioinformatics = computer science + biomedicine Discover genetic basis for disease (cancer, diabetes)Develop new diagnostic devices (cDNA chip) cDNA Array
Cellular engineering
bioimmetics
bioinformatics
![Page 8: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/8.jpg)
Genetic engineering
![Page 9: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/9.jpg)
Genetic engineering in brief
![Page 10: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/10.jpg)
GENETIC AND BIOMEDICALENGINEERING
![Page 11: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/11.jpg)
First attempt to produce insuline through rDNA technology:1970s
Human insulin gene and lac operon promoter inserted into E.coli plasmid
July 1980, 17 diabetic patients were treated with recombinant insulin at Guy’s hospital,london :Great success.
First RDT product administered to human and worked well.
Eri Lilly company received approval to market HUMan insULIN under the trade name HUMULIN
Recombinant insulin production
![Page 12: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/12.jpg)
•Insulin A and B chain inserted differently in different E.coli culture
•Selective marker:ampicilin resistant gene
•Do pure culture
•Extract insulin A & B chain seperatelyAnd Mix them.
•Functional insuline
![Page 13: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/13.jpg)
Recombinant hGH production
![Page 14: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/14.jpg)
•Glycosylated cytokines.•2 types exist
Type I (IFN-,and IFN-)Type II (IFN-)
•Cells producing IFNsPlasmacytoid DCs (major producers of IFN- and IFN- )Fibroblasts and epithelial cellsMacrophages and Th1 Cells (predominantly IFN- )
•Stimulates the action of natural killer cell•Several interferon together in a single gene construct: more efficient interferon.
Recombinant human interferon
![Page 15: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/15.jpg)
Select desired interferon genes.
cDNA from specific interferons.
Insert into vector
Vector introduced into host cell( E. coli/yeast cell)
Isolate recombinant interferon from culture medium
Hybrid interferon
![Page 16: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/16.jpg)
Why Yeast vector? yeast suitable:glycosylation of protein similar to mammalian cell.Yield is several fold higher than E. coliHybrid IFN : more reactive in function
THERAPUTIC APPLICATION IFN-α,-β,-ɣ approved for theraputic use in 1986,1993 & 1990A swiss biotechnology firm first marketed IFN- α, named (INTRON)Treatment of large number of viral disease and cancers. Like leukemia,kaposis sarcoma,bladdar cancer,head and neck cancer,renal cell carcinoma,skin cancer,multiple myloma.Other disease:AIDS,multiple sclerosis,genital warts,hepatitis C. common cold,influenza:nasal spray
![Page 17: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/17.jpg)
Recombinant hepatitis-B surface antigen production
![Page 18: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/18.jpg)
Phage therapy
![Page 19: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/19.jpg)
BIOREMEDIATION BY BACTERIA
![Page 20: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/20.jpg)
superbugNew pseudomonas strain developed by Chakrabarty and co-workers in 1970s.
Different plasmids were used and new bacterium was constructed.SUPERBUG.
US patented this SUPERBUG that can degrade a number of hydrocarbons of petrolium simultaniously.
First genetically engineered microorganism patented.
CAM-camphor degradingOCT-octane degradingXYL-xylene degradingNAH-napthalene degrading
Compatible: CAM-OCT plasmid
![Page 21: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/21.jpg)
•Biodegradative pathway gene and lux gene constructWhile degrading pollutants it gives luminiscence.thus we can Conclude that degradation is goin on
The operon of the TOL plasmid pWW0 of Pseudomonas putida encodes a set of enzymes involved in the conversion of toluene and xylenes to their carboxylic acid derivatives. The last gene of the upper operon,
Xylene and toluene(pollutant)
TOL operon of P.putida Carboxilic acid derivative(degraded form)
lux genes is present in marine bacterium, Vibrio fischeri
![Page 22: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/22.jpg)
Biodegradation along with bioluminescence
![Page 23: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/23.jpg)
![Page 24: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/24.jpg)
BIOPROCESS ENGINEERING
![Page 25: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/25.jpg)
p ppa pa
Two vector expression system for producing diametric proteinP-promoter,pa-polyadenylation seq
Recombinant protein can be produced in microorganisms .3 types of expression vectors can be used-1)Two vector expression system2)Two gene expression vector3)Dicistronic expression vector
rh- Growth Hormone, r-Human insulin, Erythropoietin,Follicle stimulating hormone, Interferon, Insulin like growth factor, Tissue Plasminogen Acivator,factor VIII,DNase, Envelope proteing of hepatitis B virus.
Produced by any of these type of method which is convenient
Expression vectors for protein production
![Page 26: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/26.jpg)
Two gene expression vector Dicistronic expression vector
![Page 27: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/27.jpg)
References
6)http://en.wikipedia.org/wiki/Biological_engineering
7)http://www.biopharminternational.com/biopharm/article/articleDetail.jsp?id=3019798)http://www.wiley-vch.de/books/biotech/pdf/v11b_gene.pdf
1)U.Satyanarayana;biotechnology;books and allied (p) ltd,p-144,191,192,197,201
2)http://bioengineering.stanford.edu/
3)http://www.biotecharticles.com/Biotechnology-products-Article/Production-of-Recombinant-Human-Growth-Hormone-Somatotropin-367.html4)www.littletree.com.au/dna.htm
![Page 28: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/28.jpg)
Questions ?....
![Page 29: Bioengineering custom microbes, genetic engineering,bioremediation,bioprocess,biomedical engineering](https://reader036.vdocuments.site/reader036/viewer/2022081715/54100ab18d7f727e0c8b45b3/html5/thumbnails/29.jpg)
THANK YOU