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Bellwork:
Homework for week of March 5th – 9th Mon. Good Luck!Tues. ISTEP
Math/L. Arts Applied SkillsWeds. ISTEP
Writing PromptThurs. Recombinant DNA Pre-Lab
Friday Modeling Recombinant DNA Lab
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Learning Goals:
Evaluate the importance of plant and animal breeding to humans.
Summarize the steps used to engineer transgenic organisms.
Analyze how mapping the human genome is benefitting human life.
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• Predict the outcome of a test cross.
Section Objectives:
• Evaluate the importance of plant and animal breeding to humans.
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1.Evaluate the importance of plant and animal breeding to humans.
2.Summarize the steps used to engineer a transgenic organism
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Chapter 13: Genetic Engineering
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Chapter 13: Genetic Engineering
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This genetically engineered plant Glows-in-the-Dark!
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A genetically engineered mouse that can grow a human ear!
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13-1 Applied Genetics
Selective Breeding: Allowing only animals with
desired traits to reproduce.
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In 1947, an average milk cow produced 4997 pounds of milk per year.
In 1997, 50 years later, an average milk cow produced 16 915 pounds of milk per year
Selective Breeding
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Hybridization
Crossing (reproducing) different individuals to bring together the best of both organisms
Produce a Hybrid which are often hardier than parents.
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Inbreeding
Breeding of individuals with similar characteristics to maintain these characteristics
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Test Cross Cross of an individual with unknown
genotype with an individual with known genotype
Used to determine genotype
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• Summarize the steps used to engineer transgenic organisms.
Learning Goal:
• Give examples of applications and benefits of genetic engineering.
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13-2: Recombinant DNA Technology
1. DNA Extraction – taking DNA out of the cell2. Restriction enzymes: are special enzymes
that cut DNA in specific locations3. Gel electrophoresis: DNA is separated and
analyzed
Some tools we use to change DNA:
Scientists use our knowledge of DNA to study and change it!
Genetic Engineering: making changes in the DNA of living organisms
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4. PCR (polymerase chain reaction): making COPIES of a particular gene that is CUT (by restriction enzymes) out of the original DNA PCR animation
5. Cutting and Pasting: we can make new DNA by combining DNA from different sources this is called Recombinant DNA
Description: DNA polymerase (blue) makes many copies of DNA (red) in a cycle of the polymerase chain reaction (PCR).
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What is…?
1. recombinant DNA
2. transgenic organism
3. restriction enzyme
4. plasmid
What is…?
1. recombinant DNA
2. transgenic organism
3. restriction enzyme
4. plasmid
Cleavage
Cut
Insertion
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DNA Fragments
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Plasmid
Plasmids are circular DNA molecules that are separate from the chromosomal DNA .
They usually occur in bacteria
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Action ofRestriction
Enzyme
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Foreign DNA into Plasmid
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Gel Electrophoresis
DNAUnique pattern created by the movement of the DNA through the gel- can identify an individual
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DNA Fingerprinting
Help Jimmy Sweet solve his candy mystery!
Who did it?
Click to Solve
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DNA Fingerprinting: Who did it
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Transformation – a cell takes & incorporates DNA from outside into its own DNA
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Steps to Transforming Bacteria1. Take the gene of interest out of the DNA from a
human cell2. Cut it out using restriction enzymes (act like
scissors!!)3. You are left with your “gene of interest”4. Take a plasmid (ring of DNA) out of a bacterial
cell, cut it with restriction enzymes.5. Place the gene of interest in the plasmid,
making a ring again6. Put this ring back into a bacteria and let it
reproduce!!
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When the same sequence of bases is found on both DNA strands, but in opposite orders, it is called a
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Transforming Plant Cells A bacterium inserts a DNA plasmid
into plant cells to create new unique plants
Ex. Plants resistant to pesticides
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Transforming Animal Cells•DNA is injected into the nucleus of an egg. As the embryo grows the new DNA will be reproduced
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Today’s Goal: Analyze how genetic engineering and
mapping the human genome will benefit future generations.
Many scientists consider genetic engineering to be simply an effective
method of selective breeding. Explain.
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Today’s Goal: Analyze how genetic engineering and
mapping the human genome will benefit future generations.
Gel Electrophoresis
PCR- Polymerase Chain Reaction
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Biotechnology New technology has created a new field
of study: Biotechnology We can create transgenic organisms –
that contain genes from other organisms
Glowing bumblebee: Modified Piglets!
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Transgenic Animals
Have been used to study genes and improve the food supply Research is under way where goats could be
modified to produce spider silk proteins in their milk that could be used in the production of medical sutures and bullet-proof vests
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Transgenic Plants Modified plants are important in our food
supply! 25% of all corn grown in US in genetically
modified Ex. They have natural insecticides, resist
herbicides, vitamins
86%
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Recombinant DNA DNA produced by
combining DNA from different sources.Genetically
engineered cells contain recombinant DNA
Firefly gene + tobacco plant = glowing tobacco plant!
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Cloning: creating genetically identical individuals
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Cloning Mimi
Click and Clone!
Genetic Science Learning Center, "Permissions Policy," Learn.Genetics, 29 April 2009, <http://learn.genetics.utah.edu/gslc/permissions.html> (29 April 2009)
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13.3- Human
Genome Project When: Began in 1990, finished in 2003 Cost: approx. $3 billion What: Sequenced the human genome (all 24
chromosomes) Why: To help understand our DNA Fun fact:
The order of almost all (99.9%) nucleotide bases is exactly the same in all people
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Applications of BiotechnologyTechnology Example Benefit
Selective Breeding
Recombinant DNA
•in medicine
•In agriculture
Gel Electrophoresis
•DNA Fingerprint
Human Genome•Gene therapy
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Applications of BiotechnologyTechnology Example Benefit
Selective Breeding
Recombinant DNA
•in medicine
•In agriculture
Gel Electrophoresis
•DNA Fingerprint
Human Genome•Gene therapy
Complete the following data table to review the benefits of Biotechnology
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Name My pts
Pts Poss. Tot “My Pts”
TPP Grade
7 Ch 12 Reading Guide
15 156
8 Ch 12 Genetics
Test40 196
Goal: Evaluate the importance of plant and animal breeding to humans.
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Today’s Goal: Describe the effect selective breeding will
have on the human population.
Place Selective Breeding lab in the collection folder.
• What is selective breeding?
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What effect might selective breeding of plants and animals
have on the size of Earth’s human population? Why?
1.
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2.
Using the corn experiment, explain what happened to farmers crops after many years of only choosing the best seeds?
Why would you want to use selective breeding?
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3.
• Using David O Conover’s fish experiment, compare and contrast the tanks that had fish getting smaller vs. larger?
• How does this explain selective breeding?
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4.• Selective breeding has many benefits but
is not without problems.
• Create a T-chart of the pros and cons of selective breeding.
Pro Con
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1. Become familiar
2. Model Recombinant DNA
3. Review and give examples of applications via 13.2 Guided Notes
Summary:
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Name My pts Pts Poss. Tot “My Pts”
TPP Grade
10 Selective Breeding
Lab
15 221
Today’s Goal:Today’s Goal:Give examples of applications and benefits
of genetic engineering.
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Relate the steps of producing recombinant DNA to the activities of the modeling procedure by explaining how the terms relate to the model
Term Lab Model
Gene insertion
Plasmid
Restriction Enzyme
Sticky ends
Recombinant DNA
Create the following Data Table
Taping Green and Red together
Green Paper
Scissors
Cut ends on Paper
Red and Green taped together
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Biology Homework for week of April 18 - 22
Mon Model Lab AnalysisEx. Credit due Weds
Tues Review Recombinant DNA
Weds Applying Genetic TechnologyReview
Th Ch. 13 Test
Fri No School-Good Friday