Download - Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008
![Page 1: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/1.jpg)
Bacteria Subsisting on Antibiotics
Gautam Dantas Church Lab
CEGS Annual Grantee Meeting - 2008
![Page 2: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/2.jpg)
Agency for healthcare research and quality, 2006
US reported MRSA infections [1000’s]
in US: MRSA kills more people than AIDS
2006: 18,964
2006: 14,627
![Page 3: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/3.jpg)
Mechanisms of antibiotic resistance
antibiotic modification efflux target modification
![Page 4: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/4.jpg)
resistance gene origin hypothesis: antibiotic resistance genes originated as
producer self immunity and was transferred to human pathogens
Proc. Nat. Acad. Sci. 70:2276 (1973)
![Page 5: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/5.jpg)
Science 312:374 (2006)
![Page 6: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/6.jpg)
many antibiotics are produced in the environment, but it is likely that they
undergo further biological transformations
![Page 7: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/7.jpg)
G. Dantas., M. Sommer et al. Science 320:100 (2008)
GROWTH NO GROWTH
Bacteria subsisting on antibiotics are widely distributed in the environment
![Page 8: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/8.jpg)
Antibiotic eaters are phylogenetically diverse
G. Dantas., M. Sommer et al. Science 320:100 (2008)
![Page 9: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/9.jpg)
microbes subsisting on antibiotics are phylogenetically
diverse
they comprise of 3 out the 4 major phyla of human microbiomes
27% of the phylogenetic nearest named isolates are
pathogens
compared to antibiotic producer organisms
they constitute a more accessible reservoir of
resistance
![Page 10: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/10.jpg)
Bacteria subsisting on antibiotics are extremely resistant
G. Dantas., M. Sommer et al. Science 320:100 (2008)
![Page 11: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/11.jpg)
G. Dantas., M. Sommer et al. Science 320:100 (2008)
![Page 12: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/12.jpg)
using functional genomic selections we have identified more than 150 unique antibiotic resistance genes
from antibiotic eaters
![Page 13: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/13.jpg)
Gentamicin resistance from eater vs. Salmonella enterica subsp. enterica serovar Typhimurium genomic island I
Experiments by: G. Dantas, M. Sommer. Unpublished data
![Page 14: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/14.jpg)
resistance genes from some antibiotic catabolizers are identical to resistance
genes of pathogens at the nucleotide level
![Page 15: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/15.jpg)
best genetic support for producer hypothesis:
>gi|27461214|gb|AY082011.1| Enterococcus faecium strain 10/96A vanD gene cluster, complete sequence; and ISEfa4 insertion sequence element
>gi|4104707:67-693 Streptomyces toyocaensis D-ala-D-ala dipeptidase (vanXst) gene, complete cds; and unknown gene
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 42: 2215 (1998), Science 312:374 (2006),
Nature Reviews Microbiology 5:175 (2007)
![Page 16: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/16.jpg)
Producer vs. Pathogen
![Page 17: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/17.jpg)
best homology between resistance gene from producer and pathogen: 66% identity @ nucleotide level
homology between resistance gene from eater and
pathogen: 98.5%, 99.9%, 99.9%, 100% identity @ nucleotide level
![Page 18: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/18.jpg)
some antibiotic resistance genes acquired by pathogens by HGT may have originated from
antibiotic eaters
![Page 19: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/19.jpg)
Acknowledgements
Morten SommerGeorge Church John AachDuhee BangJay Lee Church Lab
Rantimi Oluwasegun (Cornell)
Jay Shendure (U.W.)Lynn Bry (BWH Pathology) Roberto Kolter (HMS)Peter Girguis, Hellen White
(Harvard)Will Haas (HMS)Wayne Curtis (Penn State)David Ellison
![Page 20: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/20.jpg)
![Page 21: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/21.jpg)
publications about antibiotic resistance
![Page 22: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/22.jpg)
Experimental Design resuspend in minimal media
4x filter 500uL Innoculum
11 soils X 18 antibiotics
toothpick passage 1
toothpick passage 2
plate on solid (100,1,0.01uL, no
carbon)
liquid passage of
clones restreak
Experiments by: G. Dantas, M. Sommer, R. Oluwasegun.
![Page 23: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/23.jpg)
11 soils: pristine, farm, urban
18 antibiotics: chemical class:
beta-lactams quinolones
sulfonamides amphenicols
aminoglycosides glycopeptides
amino-acid derivorigin:
natural productssemi-synthetic
synthetic compounds
![Page 24: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/24.jpg)
Penicillin Catabolism in Action
G. Dantas., M. Sommer et al. Science 320:100 (2008)
![Page 25: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/25.jpg)
G. Dantas., M. Sommer et al. Science 320:100 (2008)
Penicillin Catabolism in Action
![Page 26: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/26.jpg)
some antibiotic resistance genes acquired by pathogens by HGT may have
originated from antibiotic eaters
![Page 27: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/27.jpg)
>gi|94960156|gb|DQ519091.1| Acinetobacter baumannii strain DB60079/01 class I integron
Experiments by: G. Dantas, M. Sommer. Unpublished data
![Page 28: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/28.jpg)
>dbj|AB207867.1| Escherichia coli transposon Tn2610, complete sequence
gi|83627312|dbj|AB207867.1|_Es TCCGGTGCGGGGACGGCTGCTCAGTGTTGCGCCTGTGTTCGCACGTTCGTGE_AB95Da04_01_mgRes1_R.trim.f ---------------------------------TCTGTTCGCACGTTCGT * ***************gi|83627312|dbj|AB207867.1|_Es ATCGGTGCGTTCTGATCTTCGCGTCAGACATTGCCGCGGCGCGGGCACAAGE_AB95Da04_01_mgRes1_R.trim.f ATCGGTGCGTTCTGATCTTCGCGTCAGACATTGCCGCGGCGCGGGCACAA **************************************************gi|83627312|dbj|AB207867.1|_Es CAAAAAGCCCGGCATCGCTGCCGGGCTCCGGCCCCGTCCTTGGGGCCTTGGE_AB95Da04_01_mgRes1_R.trim.f CAAAAAGCCCGGCATCGCTGCCGGGCTCCGGCCCCGTCCTTGGGGCCTTG **************************************************gi|83627312|dbj|AB207867.1|_Es ATGTCGGGTCGTTGCCGGGATCGGACCGCGCTGGCGCGGTCCGGTTCCCTGE_AB95Da04_01_mgRes1_R.trim.f ATGTCGGGTCGTTGCCGGGATCGGACCGCGCTGGCGCGGTCCGGTTCCCT **************************************************gi|83627312|dbj|AB207867.1|_Es GACGACCGGGCCAACCGGATCAGAAATCCATGCCGCCCATGCCGCCCATAGE_AB95Da04_01_mgRes1_R.trim.f GACGACCGGGCCAACCGGATCAGAAATCCATGCCGCCCATGCCGCCCATA **************************************************gi|83627312|dbj|AB207867.1|_Es CCGCCAGCACCCGGCATGGCCGGCTCTTCCTTCTTCGGCACTTCGGCCACGE_AB95Da04_01_mgRes1_R.trim.f CCGCCAGCACCCGGCATGGCCGGCTCTTCCTTCTTCGGCACTTCGGCCAC **************************************************gi|83627312|dbj|AB207867.1|_Es GACCACTTCGGTCGTGATCGCAAGGCCGGCGACGGAAGCGGCGTGCTGCAGE_AB95Da04_01_mgRes1_R.trim.f GACCACTTCGGTCGTGATCGCAAGGCCGGCGACGGAAGCGGCGTGCTGCA **************************************************gi|83627312|dbj|AB207867.1|_Es GGGCCGAGCGGGTCACCTTGGTCGGGTCCAGGATGCCCATGGCGATCATGGE_AB95Da04_01_mgRes1_R.trim.f GGGCCGAGCGGGTCACCTTGGTCGGGTCCAGGATGCCCATGGCGATCATG **************************************************gi|83627312|dbj|AB207867.1|_Es TCGCCGAACTCGCCGGTGGCGGCGTTGTAGCCGTAGCTGCCTTCGCCGGCGE_AB95Da04_01_mgRes1_R.trim.f TCGCCGAACTCGCCGGTGGCGGCGTTGTAGCCGTAGCTGCCTTCGCCGGC **************************************************gi|83627312|dbj|AB207867.1|_Es CTTGACGTTGGCCACGATCACGCTCGGTTCTTCACCGGCGTTGGCCACGAGE_AB95Da04_01_mgRes1_R.trim.f CTTGACGTTGGCCACGATCACGCTCGGTTCTTCACCGGCGTTGGCCACGA **************************************************gi|83627312|dbj|AB207867.1|_Es TGGCGCGCAGCGGGGCTTCCAGCGCACGGCGGGTGATGGCGATGCCCAGGGE_AB95Da04_01_mgRes1_R.trim.f TGGCGCGCAGCGGGGCTTCCAGCGCACGGCGGGTGATGGCGATGCCCAGG **************************************************gi|83627312|dbj|AB207867.1|_Es TTCTGGTCTTCGTTGATGCCCTGCCAGGGCAGATCCGTGCACAGCACCTTGE_AB95Da04_01_mgRes1_R.trim.f TTCTGGTCTTCGTTGATGCCCTGCCAGGGCAGATCCGTGCACAGCACCTT **************************************************gi|83627312|dbj|AB207867.1|_Es GCCGTAGAAGAACAGCAAGGCCGCCAATGCCTGACGATGCGTGGAGACCGGE_AB95Da04_01_mgRes1_R.trim.f GCCGTAGAAGAACAGCAAGGCCGCCAATGCCTGACGATGCGTGGAGACCG **************************************************gi|83627312|dbj|AB207867.1|_Es AAACCTTGCGCTCGTTCGCCAGCCAGGACAGAAATGCCTCGACTTCGCTGGE_AB95Da04_01_mgRes1_R.trim.f AAACCTTGCGCTCGTTCGCCAGCCAGGACAGAAATGCCTCGACTTCGCTG **************************************************gi|83627312|dbj|AB207867.1|_Es CTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGGE_AB95Da04_01_mgRes1_R.trim.f CTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACG **************************************************gi|83627312|dbj|AB207867.1|_Es AACCCAGTGGACATAAGCCTGTTCGGTTGGTAAGCTGTAATGCAAGTAGCGE_AB95Da04_01_mgRes1_R.trim.f AACCCAGTGGACATAAGCCT------------------------------ ********************
gi|83627312|dbj|AB207867.1|_Es TCCGGTGCGGGGACGGCTGCTCAGTGTTGCGCCTGTGTTCGCACGTTCGTGE_AB95Da04_01_mgRes1_R.trim.f ---------------------------------TCTGTTCGCACGTTCGT * ***************gi|83627312|dbj|AB207867.1|_Es ATCGGTGCGTTCTGATCTTCGCGTCAGACATTGCCGCGGCGCGGGCACAAGE_AB95Da04_01_mgRes1_R.trim.f ATCGGTGCGTTCTGATCTTCGCGTCAGACATTGCCGCGGCGCGGGCACAA **************************************************gi|83627312|dbj|AB207867.1|_Es CAAAAAGCCCGGCATCGCTGCCGGGCTCCGGCCCCGTCCTTGGGGCCTTGGE_AB95Da04_01_mgRes1_R.trim.f CAAAAAGCCCGGCATCGCTGCCGGGCTCCGGCCCCGTCCTTGGGGCCTTG **************************************************gi|83627312|dbj|AB207867.1|_Es ATGTCGGGTCGTTGCCGGGATCGGACCGCGCTGGCGCGGTCCGGTTCCCTGE_AB95Da04_01_mgRes1_R.trim.f ATGTCGGGTCGTTGCCGGGATCGGACCGCGCTGGCGCGGTCCGGTTCCCT **************************************************gi|83627312|dbj|AB207867.1|_Es GACGACCGGGCCAACCGGATCAGAAATCCATGCCGCCCATGCCGCCCATAGE_AB95Da04_01_mgRes1_R.trim.f GACGACCGGGCCAACCGGATCAGAAATCCATGCCGCCCATGCCGCCCATA **************************************************gi|83627312|dbj|AB207867.1|_Es CCGCCAGCACCCGGCATGGCCGGCTCTTCCTTCTTCGGCACTTCGGCCACGE_AB95Da04_01_mgRes1_R.trim.f CCGCCAGCACCCGGCATGGCCGGCTCTTCCTTCTTCGGCACTTCGGCCAC **************************************************gi|83627312|dbj|AB207867.1|_Es GACCACTTCGGTCGTGATCGCAAGGCCGGCGACGGAAGCGGCGTGCTGCAGE_AB95Da04_01_mgRes1_R.trim.f GACCACTTCGGTCGTGATCGCAAGGCCGGCGACGGAAGCGGCGTGCTGCA **************************************************gi|83627312|dbj|AB207867.1|_Es GGGCCGAGCGGGTCACCTTGGTCGGGTCCAGGATGCCCATGGCGATCATGGE_AB95Da04_01_mgRes1_R.trim.f GGGCCGAGCGGGTCACCTTGGTCGGGTCCAGGATGCCCATGGCGATCATG **************************************************gi|83627312|dbj|AB207867.1|_Es TCGCCGAACTCGCCGGTGGCGGCGTTGTAGCCGTAGCTGCCTTCGCCGGCGE_AB95Da04_01_mgRes1_R.trim.f TCGCCGAACTCGCCGGTGGCGGCGTTGTAGCCGTAGCTGCCTTCGCCGGC **************************************************gi|83627312|dbj|AB207867.1|_Es CTTGACGTTGGCCACGATCACGCTCGGTTCTTCACCGGCGTTGGCCACGAGE_AB95Da04_01_mgRes1_R.trim.f CTTGACGTTGGCCACGATCACGCTCGGTTCTTCACCGGCGTTGGCCACGA **************************************************gi|83627312|dbj|AB207867.1|_Es TGGCGCGCAGCGGGGCTTCCAGCGCACGGCGGGTGATGGCGATGCCCAGGGE_AB95Da04_01_mgRes1_R.trim.f TGGCGCGCAGCGGGGCTTCCAGCGCACGGCGGGTGATGGCGATGCCCAGG **************************************************gi|83627312|dbj|AB207867.1|_Es TTCTGGTCTTCGTTGATGCCCTGCCAGGGCAGATCCGTGCACAGCACCTTGE_AB95Da04_01_mgRes1_R.trim.f TTCTGGTCTTCGTTGATGCCCTGCCAGGGCAGATCCGTGCACAGCACCTT **************************************************gi|83627312|dbj|AB207867.1|_Es GCCGTAGAAGAACAGCAAGGCCGCCAATGCCTGACGATGCGTGGAGACCGGE_AB95Da04_01_mgRes1_R.trim.f GCCGTAGAAGAACAGCAAGGCCGCCAATGCCTGACGATGCGTGGAGACCG **************************************************gi|83627312|dbj|AB207867.1|_Es AAACCTTGCGCTCGTTCGCCAGCCAGGACAGAAATGCCTCGACTTCGCTGGE_AB95Da04_01_mgRes1_R.trim.f AAACCTTGCGCTCGTTCGCCAGCCAGGACAGAAATGCCTCGACTTCGCTG **************************************************gi|83627312|dbj|AB207867.1|_Es CTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGGE_AB95Da04_01_mgRes1_R.trim.f CTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACG **************************************************gi|83627312|dbj|AB207867.1|_Es AACCCAGTGGACATAAGCCTGTTCGGTTGGTAAGCTGTAATGCAAGTAGCGE_AB95Da04_01_mgRes1_R.trim.f AACCCAGTGGACATAAGCCT------------------------------ ********************
gi|83627312|dbj|AB207867.1|_Es TCCGGTGCGGGGACGGCTGCTCAGTGTTGCGCCTGTGTTCGCACGTTCGTGE_AB95Da04_01_mgRes1_R.trim.f ---------------------------------TCTGTTCGCACGTTCGT * ***************gi|83627312|dbj|AB207867.1|_Es ATCGGTGCGTTCTGATCTTCGCGTCAGACATTGCCGCGGCGCGGGCACAAGE_AB95Da04_01_mgRes1_R.trim.f ATCGGTGCGTTCTGATCTTCGCGTCAGACATTGCCGCGGCGCGGGCACAA **************************************************gi|83627312|dbj|AB207867.1|_Es CAAAAAGCCCGGCATCGCTGCCGGGCTCCGGCCCCGTCCTTGGGGCCTTGGE_AB95Da04_01_mgRes1_R.trim.f CAAAAAGCCCGGCATCGCTGCCGGGCTCCGGCCCCGTCCTTGGGGCCTTG **************************************************gi|83627312|dbj|AB207867.1|_Es ATGTCGGGTCGTTGCCGGGATCGGACCGCGCTGGCGCGGTCCGGTTCCCTGE_AB95Da04_01_mgRes1_R.trim.f ATGTCGGGTCGTTGCCGGGATCGGACCGCGCTGGCGCGGTCCGGTTCCCT **************************************************gi|83627312|dbj|AB207867.1|_Es GACGACCGGGCCAACCGGATCAGAAATCCATGCCGCCCATGCCGCCCATAGE_AB95Da04_01_mgRes1_R.trim.f GACGACCGGGCCAACCGGATCAGAAATCCATGCCGCCCATGCCGCCCATA **************************************************gi|83627312|dbj|AB207867.1|_Es CCGCCAGCACCCGGCATGGCCGGCTCTTCCTTCTTCGGCACTTCGGCCACGE_AB95Da04_01_mgRes1_R.trim.f CCGCCAGCACCCGGCATGGCCGGCTCTTCCTTCTTCGGCACTTCGGCCAC **************************************************gi|83627312|dbj|AB207867.1|_Es GACCACTTCGGTCGTGATCGCAAGGCCGGCGACGGAAGCGGCGTGCTGCAGE_AB95Da04_01_mgRes1_R.trim.f GACCACTTCGGTCGTGATCGCAAGGCCGGCGACGGAAGCGGCGTGCTGCA **************************************************gi|83627312|dbj|AB207867.1|_Es GGGCCGAGCGGGTCACCTTGGTCGGGTCCAGGATGCCCATGGCGATCATGGE_AB95Da04_01_mgRes1_R.trim.f GGGCCGAGCGGGTCACCTTGGTCGGGTCCAGGATGCCCATGGCGATCATG **************************************************gi|83627312|dbj|AB207867.1|_Es TCGCCGAACTCGCCGGTGGCGGCGTTGTAGCCGTAGCTGCCTTCGCCGGCGE_AB95Da04_01_mgRes1_R.trim.f TCGCCGAACTCGCCGGTGGCGGCGTTGTAGCCGTAGCTGCCTTCGCCGGC **************************************************gi|83627312|dbj|AB207867.1|_Es CTTGACGTTGGCCACGATCACGCTCGGTTCTTCACCGGCGTTGGCCACGAGE_AB95Da04_01_mgRes1_R.trim.f CTTGACGTTGGCCACGATCACGCTCGGTTCTTCACCGGCGTTGGCCACGA **************************************************gi|83627312|dbj|AB207867.1|_Es TGGCGCGCAGCGGGGCTTCCAGCGCACGGCGGGTGATGGCGATGCCCAGGGE_AB95Da04_01_mgRes1_R.trim.f TGGCGCGCAGCGGGGCTTCCAGCGCACGGCGGGTGATGGCGATGCCCAGG **************************************************gi|83627312|dbj|AB207867.1|_Es TTCTGGTCTTCGTTGATGCCCTGCCAGGGCAGATCCGTGCACAGCACCTTGE_AB95Da04_01_mgRes1_R.trim.f TTCTGGTCTTCGTTGATGCCCTGCCAGGGCAGATCCGTGCACAGCACCTT **************************************************gi|83627312|dbj|AB207867.1|_Es GCCGTAGAAGAACAGCAAGGCCGCCAATGCCTGACGATGCGTGGAGACCGGE_AB95Da04_01_mgRes1_R.trim.f GCCGTAGAAGAACAGCAAGGCCGCCAATGCCTGACGATGCGTGGAGACCG **************************************************gi|83627312|dbj|AB207867.1|_Es AAACCTTGCGCTCGTTCGCCAGCCAGGACAGAAATGCCTCGACTTCGCTGGE_AB95Da04_01_mgRes1_R.trim.f AAACCTTGCGCTCGTTCGCCAGCCAGGACAGAAATGCCTCGACTTCGCTG **************************************************gi|83627312|dbj|AB207867.1|_Es CTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGGE_AB95Da04_01_mgRes1_R.trim.f CTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACG **************************************************gi|83627312|dbj|AB207867.1|_Es AACCCAGTGGACATAAGCCTGTTCGGTTGGTAAGCTGTAATGCAAGTAGCGE_AB95Da04_01_mgRes1_R.trim.f AACCCAGTGGACATAAGCCT------------------------------ ********************
Experiments by: G. Dantas, M. Sommer. Unpublished data
![Page 29: Bacteria Subsisting on Antibiotics Gautam Dantas Church Lab CEGS Annual Grantee Meeting - 2008](https://reader030.vdocuments.site/reader030/viewer/2022032800/56649d2b5503460f949ffe11/html5/thumbnails/29.jpg)
>gi|4583495|gb|AF133140.1| Pseudomonas sp. plasmid pPSTG2
Experiments by: G. Dantas, M. Sommer. Unpublished data