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A centrifugal lab-on-a-disc device for the in situ determination of dissolved
reactive phosphate in water
Gillian Duffy, Brendan Heery, Ivan Maguire, Charles Nwankire, Jens Ducrée, Fiona Regan 1
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Contents Background information
Centrifugal microfluidics
Analytical method on disc
Detection system
Integrated system
2
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Phosphorus in water Growth limiting nutrient:
• algal bloom
• hypoxic waters
• death of aquatic animals
• toxic blooms
3 http://serc.carleton.edu/microbelife/topics/redtide/general.html
http://www.natureworldnews.com/articles/15693/20150717/toxic-algae-expected-lake-erie-potentially-worst.htm
http://sites.duke.edu/biology217_01_s2011_mkg14/ecological-impacts/eutrophication/
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Traditional analysis
Grab sample
Travel
Storage
Analysis
Travel
4
• Time intensive
• Labour intensive
• Expensive
instrumentation
• Low sampling
frequency
• Contamination
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Centrifugal microfluidics
5
Centrifugal force
Direction of rotation
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Analytical method
6
Four methods:
High sensitivity
Selectivity
Simplicity
LOD and LOQ
Linear range
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Analytical method
7
R² = 0.9983
R² = 0.9583
R² = 0.9377
R² = 0.9872 0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
0 100 200 300 400 500 600
Ab
sorb
an
ce (
AU
)
Concentration (µg L-1 PO4-P)
0
0.0002
0.0004
0.0006
0.0008
0.001
0.0012
0.0014
0.0016
0.0018
Se
nsi
tiv
ity
(A
U (
µg
L-1)-1
)
= ascorbic acid method
= stannous chloride method
= stannous chloride-hydrazine method
= yellow method
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Analytical method
8
PO43- + 2(NH4)6Mo7O24.4H2O
𝑯𝟐𝑺𝑶𝟒 H3PMo12O40
1.
H3PMo12O40 + 2C6H8O6 + C8H4K2O12Sb2.3H2O
H7PMoV
4MoVI8O40 + 2C6H6O6 + 4H+ 2.
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Detection system
9
increase
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Microfluidic disc
10
Reservoir
Microchannel
Air vent
Detection chamber
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11
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Detection system
12
Roithner 880 nm laser Radionics 880 nm LED
Radionics photodiode
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Optical path length study
LED (B)
Laser (A)
Photodiode B
Photodiode A
Optical path length 13
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Optical path length study
R² = 0.9809
R² = 0.9985
-0.001
0
0.001
0.002
0.003
0.004
0.005
0.006
0.007
0 20 40 60 80 100
Se
nsi
tiv
ity
(AU
L µ
g-1)
Optical path length (mm)
Laser
LED
14
R² = 0.9098
R² = 0.796
0
0.001
0.002
0.003
0.004
0.005
0.006
0 20 40 60 80 100 120
Se
nsi
tiv
ity
(AU
L µ
g-1)
Optical path length (mm)
LED
Laser
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Integrated NutriSense system
15
Black 3D printed container
Motor to be incorporated for disc rotation
Optical detection system is aligned on the stage
Electronics stored beneath, away from wet chemistry
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Conclusion Analytical method
Disc design optimised Mixing
bubble formation
Optical path length
Integrated system designed – first prototype fabricated
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Future work Incorporate motor into system
Investigate system robustness in situ
Adapt disc design for other analytes for water quality analysis
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Acknowledgements Brendan Heery
Ivan Maguire
Prof. Fiona Regan
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Thank you! 19