PATENT

pie. The composition comprises an ester capable of producing a detectable response upon interac- tion with said analyte, and a diazonium salt having the structure See Patent for Chemical Structure in which A£31 + 0 + L is an anion, and R same or differ, is H, lower alkyl, aryl, or both of R together form a fused ring system, and in which R+40 +0 is H, OH or lower alkyl. + RE. + REkyl. + REkyl. + REn anion, and R same or differ, is H, lower alkyl, aryl, or both of R together form a fused ring system, and in which R+40 +0 is H, OH or lower alkyl. + RE

4639420

M E T H O D F O R T H E I M M U N O A N A L Y S I S O F

C H O L E S T E R O L E P O X I D E S

ABSTRACTS 165

An apparatus for the production of biocatalyst beads Comprises an immobilization vessel (10) which is provided at its lower end with discharge pipes (23) which extend through a pressure chamber (25) in which a gas pressure prevails, The pressure chamber (25) is terminated by a plate (24) exhibiting holes which coaxially sur- round the discharge pipes (23). At the lower end of the immobilization vessel (10) is provided a connecting nozzle (30) which is connected in ger- mproof sealing manner to the wall of a collecting vessel (11) which contains a solution of a cross- linking agent or a reprecipitation bath. The col- lecting vessel (11) may be constructed as a fermenter and provided with an agitator. Both of the vessels may be jointly sterilized.

Carl P Schaffner

An immunoassay is provided for cholesterol epoxide. To prepare the antibodies used in the immunoassay, novel immunogens, are prepared which comprise a 3,5(6)-transdiaxial- dihydroxycholestane-6(5)-yl-hapten adduct linked to a covalently bonded bridge to a carrier protein. To detect cholesterol epoxide in the sample, it is converted to the hapten adduct, then contacted with the selected antibody.

4639421

F L U O R E S C E N T G R A M S T A I N

Burton H Sage assigned to Becton Dickinson and Company

A method to determine the Gram sign of micro- organisms includes staining the microorganisms with a plurality of fluorescent dyes in the pre- sence of a staining buffer, applying excitation energy to the stained microorganisms, observing the color of the fluorescence emission of the stained microorganisms, and assigning the posi- tive Gram sign to microorganisms which fluoresce substantially green and the negative Gram sign to microorganisms which fluoresce substantially orange.

4639423

A P P A R A T U S F O R T H E P R O D U C T I O N O F B I O C A T A L Y S T

B E A D S

Wolfgang Kahlert, Joachim Klein, Hans-Jurgen Steinert, Claus Vorlop, Melsungen, Federal Republic Of Germany assigned to lntermedicat GmbH

4639425

H E T E R O G E N E O U S I M M U N O A S S A Y A N D R E A G E N T

T H E R E F O R E

Manfred Baier, Pocking Possenhofen, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH

The invention is an improvement in a hetero- geneous immunoassay, and reagent therefore, for the determination of a substance in a blood serum or plasma sample, such as a parnter of an immune reaction, in which one of the reaction partners is adsorbed on a solid carrier, the im- provement comprises adding a detergent to the incubation medium of the reaction, in an amount of 0.0001 to 0.01% (w/v) to reduce inter- ferences from the serum or plasma.

4640893

N O V E L R H O D A M I N E D E R I V A T I V E S AS F L U O R O G E N I C S U B S T R A T E S F O R P R O T E I N A S E S

Walter F Mangel, Stephe Leytus, L Le Melhado assigned to University of Illinois

The present invention relates to a new class of novel Rhodamine derivatives. These derivatives which are bisamide substitution products are nonfluorescent. Whereas the monoamide sub- stitution products exhibit high fluorescence. Cleavage of a single specified amide bond by