• The platform enables vessel formation with endothelial cells• Method for pericyte-endothelial co-culture• Vasculature can be perfused, mimicking the flow observed in vivo• The high throughput platform will allow us to develop screening assays
Conclusions & Perspectives
Aim
Recreate vasculature found in vivo in a high throughput in vitro platform.
This poster shows tube formation of endothelial cells surrounded by pericytes.
Perfusable vasculatureon a High-Throughput Microfluidic Platform1 Division of Analytical Biosciences LACDR, Leiden University, the Netherlands2 Mimetas BV, the Netherlands3 Department of Nephrology, Leiden University Medical Center, the Netherlands
V. van Duinen1, S.J. Trietsch1,2, J.M. van Gils3, A.J. van Zonneveld3, T. Hankemeier1 and P. Vulto2
Pericyte-covered vasculature
• Perfusable with Dextran 150 kDa• Quantifiable barrier function
• Co-culture to create double tubulus• Endothelium surrounded by pericytes
FITC-Dextran 150 kDa
vvHUVEC-RFP
vvHUVEC-RFP
A-B) Max projection and slice of the pericyte-endothelialco-culture C) 40x z-stack D) 3D view E) 16 perfusable vesselsF) Endothelial-pericyte vessel G) Perfusion with FITC-Dextran
• 3D vessel formation in vitro• Reproducible vessel formation
Perfusion
The OrganoPlate®
• Easy to operate• 96 tissues per plate
• Stand alone, pump-free perfusion
• Phaseguide-based tissue patterning
• 3D cell culture embedded in ECM
• Easy to operate• Phaseguide-based tissue patterning
Contact details
vvHUVEC-RFPPericytes
Seeding protocol
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vvHUVEC-RFPPericytes
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vvHUVEC-RFPPericytes
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