Clostridium perfringens enterotoxin E110D Purification and crystallization of Clostridium perfringens enterotoxin mutant E110D

16619016

Fig.2 CPE

CPE

CPE&CsSoaking

Fig.1 CPECs

SPring-8 BL41XU

Clostridium perfringens enterotoxin : CPECPE31935 kDaNCCPECPE4 37 Glu94Glu110AspGlyAsp48AlaCPECPEXXAFS (X)nickel-NTA Affinity column chromatography (3720hr)(OD600=0.61 mM IPTG2518hr)

D48A-E94D

CPE12 mg/ml 295K

0.1 M CsClO/N(over night)

E94D

(E94D,E110D)0.1M HEPES pH7.015% PEG4000E110D

0.1M Sodium acetate0.1M HEPES pH7.512% PEG4000D48A

(D48A)0.1M Sodium acetate0.1M HEPES pH7.512% PEG4000E94DFig.5 XAFS(CPE-E94D)CsCPE-E94D()XAFSCsedgepeakremotepeakremoteCsCPE-E110D()CsE94DCsCsCPE-E94D

Results & DiscussionManufacture of a new cesium ion scavenger using a toxin having molecular switch ability

Fig.3 CPE-D48A-E94DNative-PAGE

MethodIntroduction

ConclusionAcknowledgment66242480720146MW(kDa)Asp94Asp94Cs (K0.3445 ) Glu110Asp94Glu110Glu110Glu110Glu110Glu110Glu110Glu110Glu110

Fig.4 CPEFig.6(CPE-E94D) Fig.7 peakFig.8 remote

Cs

1