Download - 第八章 克隆基因的表达 Expression of Cloned Genes
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第八章 克隆基因的表达Expression of Cloned Genes
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Gene Organization
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Gene Expression and Regulation Levels
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The lac Operon
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Structure and expression of the mammalian β-globin gene
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Transcription and Translation
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Transcription in Prokaryotes
Structure of a typical promoter in E.coli
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S-D Recognition Sequences in Prokaryotic mRNAs
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Termination of RNA Chains
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Expression Vector
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Secretion Cloning Vector
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Fusional ExpressionVector
Factor Xa---Activated form of factor x that participates in both the intrinsic and extrinsic pathways of blood coagulation. It catalyses the conversion of prothrombin to thrombin in conjunction with other cofactors.
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Conserved Sequences in Promoters
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Distance of Structure Genes and Promoters
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Gene expression
level and secondary
structure of mRNA
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Alteration of the Distance between Gene and Promoter
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Translation Reading Frame
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Construction the Reading Frame
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Induced Expression of Gene
• Chemical---lac promoter and IPTG
IPTG 即 Isopropyl β-D-1-thiogalactopyranoside ,也称 Isopropyl β-D-thiogalactoside ,中文名为异丙基 -β-D- 硫代半乳糖苷。分子式为 C9H18O5S ,分子量为 238.30 。
-IPTG, cell growth
+IPTG, target gene expression
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Induced Expression of Gene
• Physical---λPL and temperature sensitive(λcI875 lysogen,32 ,42℃ ℃)
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Induced Expression of GenePromoter: λPLλPR Terminator: rrnBT1T2Supression: 32 Gene expression: ℃ 42 ℃
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Transcription
and Translation
in SV40 Virus
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SV40 Virus Vector
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SV40 Virus
Vector and
COS Cells
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Expression Vectors for Mammalian Cells
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Gene Expression
in Mammals