do’s and don’ts - sciex...do’s and don’ts of oligonucleotide analysis do use columns with...

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Do’s and Don’ts of Oligonucleotide Analysis Do use columns with high pH and high temperature stability Do dedicate an LC for oligo analysis Do optimize collision energy Do re-equilibrate columns with at least 5 column volumes Do adopt a consistent cleaning protocol for your MS and LC to prevent adduct formation Do verify system performance with a system suitability standard Don’t use bottled HPLC water Don’t use MS scan range below 400 m/z Don’t use low quality reagents Don’t assume all charge states fragment equivalently Don’t use mobile phase for more than 1 day Don’t use a high source temperature AB Sciex is doing business as SCIEX. © 2019 AB Sciex. For Research Use Only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. RUO-MKT-07-9822-A

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Page 1: Do’s and Don’ts - SCIEX...Do’s and Don’ts of Oligonucleotide Analysis Do use columns with high pH and high temperature stability Do dedicate an LC for oligo analysis Do optimize

Do’s and Don’ts of Oligonucleotide Analysis

Do use columns with high pH and high temperature stability

Do dedicate an LC for oligo analysis

Do optimize collision energy

Do re-equilibrate columns with at least 5 column volumes

Do adopt a consistent cleaning protocol for your MS and LC to prevent adduct formation

Do verify system performance with a system suitability standard

Don’t use bottled HPLC water

Don’t use MS scan range below 400 m/z

Don’t use low quality reagents

Don’t assume all charge states fragment equivalently

Don’t use mobile phase for more than 1 day

Don’t use a high source temperature

AB Sciex is doing business as SCIEX. © 2019 AB Sciex. For Research Use Only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of AB Sciex Pte.

Ltd. or their respective owners. AB SCIEX™ is being used under license. RUO-MKT-07-9822-A

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