dna/rna metabolism blackburn & gait, ch. 5 neidle, ch. 4 recombination understand basics of the...
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![Page 1: DNA/RNA Metabolism Blackburn & Gait, Ch. 5 Neidle, Ch. 4 Recombination understand basics of the reaction know advantages/disadvantages of recombination](https://reader035.vdocuments.site/reader035/viewer/2022072005/56649cda5503460f949a42bd/html5/thumbnails/1.jpg)
DNA/RNA Metabolism
Blackburn & Gait, Ch. 5Neidle, Ch. 4
Recombination• understand basics of the reaction• know advantages/disadvantages of recombination• understand how recombinase protein works• know structure of Holliday junction (X-shaped)• know different types of recombination
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Recombination
• Enables the limited exchange of genetic material between paired chromosomes
• Occurs between intact double helices
• Damage to DNA can stimulate recombination
• Enables the immune system to generate a diversity of protein antibodies from a limited set of genes
• Enables viruses to integrate their genetic material into a host’s genome
• Enables host organism to assort alleles (differing copies of same gene) into novel groups - favorable & unfavorable alleles can be shuffled randomly
• Enables repair of a damaged gene in an otherwise favorable chromosome
• Enables regulation of gene expression
• Enables rearrangement of antibody genes
• Can lead to cancers - example: Burkitt’s lymphoma caused by translocation between ch. 8 and 14
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Recombination
• THREE main categories
1. Homologous recombination
takes place between similar sequences
2. Site-specific recombination
Limited sequence similarity between recombining DNAs
3. Transposition
movement of a DNA element from one position to another, little sequence similarity needed
called “illegitimate recombination”
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1. Homologous Recombination
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1. Homologous Recombination
Recombinase protein
• One example is that of Cre recombinase from a bacteriophage (virus that infects bacteria)
• The enzyme mediates strand cleavage and exchange between two pieces of DNA
• An intermediate (half reacted) configuration of the DNA is called a Holliday junction (X-shaped)
![Page 6: DNA/RNA Metabolism Blackburn & Gait, Ch. 5 Neidle, Ch. 4 Recombination understand basics of the reaction know advantages/disadvantages of recombination](https://reader035.vdocuments.site/reader035/viewer/2022072005/56649cda5503460f949a42bd/html5/thumbnails/6.jpg)
1. Homologous Recombination
Recombinase protein
![Page 7: DNA/RNA Metabolism Blackburn & Gait, Ch. 5 Neidle, Ch. 4 Recombination understand basics of the reaction know advantages/disadvantages of recombination](https://reader035.vdocuments.site/reader035/viewer/2022072005/56649cda5503460f949a42bd/html5/thumbnails/7.jpg)
1. Homologous Recombination
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2. Site-specific Recombination
Lambda phage integration and excision
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2. Site-specific Recombination
VDJ rearrangement in antibody production
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3. Transposition - Recombination
Bacterial antibiotic resistance
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3. Transposition - Recombination
Eukaryotic transposable elementsBarbara McClintock - Noble prize
Normal maizeActive C causes
synthesis of purple pigment
Mutant maizeMutant C
Ds insertion inactivates C and prevents pigment
synthesis
Mutant maizeAc allows Ds to
transpose out of C in many cells, results in
purple pigmented spots on kernels