dna denaturation by: -heat -chemicals (e.g. formamide) -high ph
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Topic 2, Nucleic acids, genes, and genomes. DNA Denaturation by: -Heat -Chemicals (e.g. formamide) -High pH Melting Temperature is dependent on: -Length of DNA (for short fragments) -Base composition. Kinetics of Renaturation. *. *. highly repetitive DNA – very low C o t. - PowerPoint PPT PresentationTRANSCRIPT
DNA Denaturation by:
-Heat
-Chemicals (e.g. formamide)
-High pH
Melting Temperature is dependent on:
-Length of DNA (for short fragments)
-Base composition
Topic 2, Nucleic acids, genes, and genomes
Kinetics of Renaturation
*
*
highly repetitive DNA –very low Cot
repetitive DNA –low Cot
non-repetitive DNA –higher Cot
0 2 4
Yeast artificial chromosome
Bacterial artificial chromosome
Sequence tagged sites
Mapping with STSs
Figure 24.11
“Shotgun sequencing and BAC walking”
Physical distance versus genetic map distance
What explains the conservation of gene order over large evolutionary distance?
Figure 1: Neurospora has a low proportion of genes in multigene families.
Figure 2: Neurospora possesses few highly similar genes.
Figure 3: Example of lack of recent duplications in a Neurospora gene family (MFS sugar transporters).
Figure 4: Correspondence between predicted RIP, methylation and repetitive DNA.
Figure 5: Overview of major intracellular signaling pathways in Neurospora.