dna & nanoscience
TRANSCRIPT
Oligonucleotides
RNA DNA
A-Form B-Form
O
HO OH
BaseHO
Ribose
O
HO
BaseHO
2’-Deoxyribose
Images from Wikipedia
Biological Roles of DNA & RNA
DNA RNA
Store genetic information
Transport information (mRNA)
Build proteins (tRNAs, Ribosomes)
Control gene expression (miRNAs,
piRNAs, siRNAs)
Store genetic information (Viral RNAs)
Catalyze reactions (Ribozymes)
Structural Features of DNA
2 nanometers
Major
Groove
Minor
Groove
1 turn = 10 bases pairs
3.4 nanometers
NN
NH2
ON
NN
N
O
NH2
H
Watson-Crick Base pairs
N
NN
N
NH2
NN
O
O
H
Adenine Thymine
Guanine Cytosine
Preparation of Oligonucleotides
PCR
Thermal Cycler
(PCR)
DNA
Plasmid DNA
Primers, triphosphates,
and Enzymes
oligonucleotides
Taq DNA Polymerase ATP GTP
CTP TTP
triphosphates
DNA Fingerprinting
http://www.pleasanton.k12.ca.us/avhsweb/thiel/bio/labs/csi.html
CSI - Forensic Investigation